Mosquitocidal properties of Solanum trilobatum L. (Solanaceae) leaf extracts against three important human vector mosquitoes (Diptera: Culicidae) (original) (raw)
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© Triveni Enterprises, Lucknow (India), 2009
Larvicidal efficacy of the aqueous and methanol extracts from green unripe and yellow ripe fruits, seeds, leaves and roots of the plant Solanum xanthocarpum was evaluated against Anopheles culicifacies, Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus, the important mosquito vectors prevalent in the arid region. Studies were carried out on late 3 rd or early 4 th instar larvae of these species using standard WHO technique. Based on concentration mortality data LC 50 and LC 90 values along with their fiducial limits, regression equation, chisquare (λ 2)/ heterogeneity of the response have been determined by log probit regression analysis. The 24 hr LC 50 values as observed for aqueous extracts for green unripe and yellow ripe fruits were 112.7, 498.2 and 846.3 mgl-1 and 104.7, 267.7 and 832.2 mg l-1 for Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus respectively. However, the 24 hr LC 50 values for methanol extracts of yellow ripe fruits and seeds were 51.6, 52.2, 118.3 and 157.1 mg l-1 and 66.9, 73.7, 123.8 and 154.9 mg l-1 for the above four vector species respectively. The results showed that larvae of anophelines were much more susceptible as compared to culicines to both the aqueous and methanol extracts from fruits and seeds of the plant tested. The methanol extracts were found 2-5 times more effective as compared to the aqueous extracts. The chi-square values calculated during the analysis did not show any heterogeneity of the response. Experiments conducted with aqueous and methanol extracts from leaves and roots of this plant species didn't show any larvicidal activity against any of the mosquito species tested. The study would be of great importance while formulating vector control strategy based on alternative plant based insecticides.
A Review on Mosquitocidal Activity of the Genus Solanum
International Journal of Zoological Investigations
As a vector, mosquitoes are responsible for transmitting deadly diseases such as Chikungunya fever, filariasis, encephalitis, West Nile Virus infection, malaria, yellow fever, dengue fever, etc. To combat this problem, various techniques and strategies had been adopted to control the mosquito population. An alternative mosquito control strategy was available from ancient times that is the application of active toxic agents from plant extracts. This preference has several advantages over the consistent application of synthetic insecticides which cause resistance in vector species, harmful effects on environment and non-target organisms, including human health. From ancient times, plants have been used to control the vector mosquitoes, and still now, in many parts of the world, many researchers are looking for plant substances to kill the mosquitoes or cause repellency in mosquitoes. The phytochemicals that originate from the plant showed various activities such as larval mortality, d...
The Indian journal of medical research, 2009
Aqueous and organic solvent extracts of plants/plant parts were effective in killing the mosquito larvae. Comparative efficacy of the aqueous and hexane extracts of dried fruit of Solanum nigrum was tested against five laboratory colonized strains of mosquito species, namely Anopheles culicifacies species A, An. culicifacies species C, An. stephensi, Culex quinquefasciatus and Aedes aegypti to assess the possibility for use of these extracts for their control. Concentrations of aqueous extract of dried fruit in the range of 62.5 to 2000 ppm and hexane extract of dried fruit in the range of 0.781 to 150 ppm were used in bioassays. The mortality data were subjected to log probit regression analysis to determine the median lethal concentrations (LC(50) and LC(90)) to kill 50 and 90 per cent of the treated larvae of the respective species. All the five species registered 100 per cent mortality in larval bioassays at 1000 ppm with aqueous extract and at 100 ppm with hexane extract of dri...
Journal of Biological Control, 2022
Mosquitoes play a key role in the transmission of diseases such as malaria, yellow fever, Japanese encephalitis, etc. Plant based compounds form alternate source of control measures against mosquitoes, in view of deleterious effects of chemical pesticides. In the present study, insecticidal activity of aerial part of Solanum torvum (Sw.) was studied against Aedes aegypti (L.) under laboratory conditions. GC-MS study was analysed in hexane, ethyl acetate and methanol extract of S. torvum and the leaf extracts yielded around 57 compounds. In the larvicidal and adulticidal tests against A. aegypti, mortality rate increased with the increased concentrations of S. torvum extract. Highest larval mortality was obtained with ethyl acetate 100% extract at the dose of 200 ?g/ml after 48 hrs experiment, followed by methanol 64% and hexane 42% leaf extract. The LC50 values of leaf extract was observed as 159.594 ?g/mL, 182.272 ?g/mL at 24 hrs interval and 85.2833 ?g/mL, 138.472 ?g/mL 48 hrs int...
Biological Activities of Solanaceous Plants Against Vector Mosquitoes: A Review
2016
Malaria, dengue, chickungunya, filariasis and Japanese encephalitis which are transmitted by mosquitoes cause thousands of deaths in India. Hence, effective mosquito control is required to enhance the health and quality of human life through the reduction of mosquito population. Therefore there is a growing need to use clean non-toxic, eco-friendly biopesticides. In this review we summarize, the current knowledge of the mosquito larvicidal activity by using different plant part extracts of Solanaceae family. Various aspects such as, instar specificity, solvents used during extraction, nature of active ingredient and promising advances made in biological control of mosquito larvae from Solanaceae plant derived secondary metabolites are also discussed . The results of the review reveal that the use of Solanaceous plant extracts have the potential to be utilized as a good, easily available, biodegradable, non-toxic and in having eco-friendly approach for the control of mosquito populat...
Mosquito larvicidal activity of Solanum nigrum berry extracts
The Indian Journal of Medical Research
Phytochemicals are widely used as biocontrol agent against vector mosquitoes. The present study was undertaken to isolate and evaluate the mosquitocidal activity of various extracts of berries of S. nigrum against Culex quinquefasciatus. Crude and chloroform: methanol (1:1, v/v) extracts of fresh, mature, green berries of S. nigrum were tested against Cx. quinquefasciatus. The lethal concentration was determined and the chemical nature of the active substance was evaluated. A qualitative phytochemical analysis of chloroform: methanol (1:1, v/v) extract was performed in search of the active ingredient. The appropriate lethal concentrations at 24 h for chloroform: methanol (1:1, v/v) extract was also studied on non-target organisms. In a 72 h bioassay experiment with crude extract, the highest mortality was recorded in 3 per cent extract. In the chloroform: methanol (1:1, v/v) solvent extract, the maximum mortality was recorded at a concentration of 120 μg/ml. The log probit analysis ...
Parasitology Research
Mosquito-borne diseases have an economic impact, including loss in commercial and labor outputs, particularly in countries with tropical and subtropical climates; however, no part of the world is free from vector-borne diseases. In mosquito control programs, botanical origin may have the potential to be used successfully as eggs, larvae, and adult. The larvicidal, ovicidal, and repellent activities of crude benzene and ethyl acetate extracts of leaf of Ervatamia coronaria and Caesalpinia pulcherrima were assayed for their toxicity against three important vector mosquitoes, viz., Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus (Diptera: Culicidae). The larval mortality was observed after 24 h of exposure. All extracts showed moderate larvicidal effects; however, the highest larval mortality was found in benzene extract of E. coronaria against the larvae of Anopheles Stephensi, Aedes aegypti, and Culex quinquefasciatus with the LC50 and LC90 values were 79.08, 89.59, and 96.15 ppm and 150.47, 166.04, and 174.10 ppm, respectively. Mean percent hatchability of the ovicidal activity was observed 48 h posttreatment. The percent hatchability was inversely proportional to the concentration of extract and directly proportional to the eggs. The leaf extract of E. coronaria was found to be most effective than Caesalpinia pulcherrima against eggs/egg rafts of three vector mosquitoes. For E. coronaria, the benzene extract exerted 300, 250, and 200 ppm against Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus, respectively. The results of the repellent activity of benzene and ethyl acetate extract of E. coronaria and Caesalpinia pulcherrima plants at three different concentrations of 1.0, 2.5, and 5.0 mg/cm2 were applied on skin of fore arm in man and exposed against adult female mosquitoes. In this observation, these two plant crude extracts gave protection against mosquito bites without any allergic reaction to the test person, and also, the repellent activity is dependent on the strength of the plant extracts. These results suggest that the leaf solvent plant extracts have the potential to be used as an ideal ecofriendly approach for the control of mosquitoes. This is the first report on the mosquito larvicidal, ovicidal, and repellent activities of the reported E. coronaria and Caesalpinia pulcherrima plants.
Alexandria Science Exchange Journal: An International Quarterly Journal of Science Agricultural Environments, 2013
The effect of methanolic plant extracts from Solanum nigrum, Acokanthera spectabilis and Heliotropium aegyptiacum were investigated on the larval mortality and development of Culex pipiens L. Also, the effects of the LC 50 of the three plant extracts on the larval haemogram were studied. Plant extracts exhibited variable bioactivities. The greatest activity was observed for S. nigrum which showed LC 50 values of 130.8 ppm after 48 hr of exposure, respectively. Percentage of larval mortality was 67.5% in the treatment with extracts of S. nigrum and 66.5% in case of A. spectabilis at 500ppm. Egg hatchability was not significantly reduced in all S. nigrum concentrations. All concentrations of the plant extracts from S. nigrum, A. spectabilis and H. aegyptiacum caused significant hindrance to the subsequent larval development and reduced both pupation and adult emergence. Drastic retardation of development was shown by S. nigrum extracts, where only 18.1% and 8.5% of the larval managed to reach pupal and adult stages, respectively, when reared in 100 ppm of the extract. However, A. spectabilis and H. aegyptiacum were more effective at higher concentrations. Moreover, the effects of the LC 50 of tested plant extracts caused a reduction in the number of haemocytes and also markedly decreased the haemocytes surface areas. Application of such plant extracts to mosquito breeding site may have great practical importance in relation to non-synthetic chemical control of this serious disease vectors.
The Indian journal of medical research, 2014
Indiscriminate use of synthetic pesticides has created serious problem for the aquatic flora and fauna, and also resulted in appearance of pesticide resistance in vector population. This study was designed to evaluate the biocontrol efficacy of aqueous and solvent extracts of mature leaves of Solanum nigrum L., against fresh water snail Lymnaea acuminata f. rufescens (Gray) (an intermediate host of parasites causing fasciolopsiasis) and larvae of Culex vishnui group (Reuben) (vector of Japanese encephalitis). Aqueous and solvent extracts of fresh, mature, green/shed dried leaves of S. nigrum were tested against adult L. acuminata and larvae of Cx. vishnui group. The lethal concentration was determined and the appropriate lethal concentration at 24 h of benzene extract was also studied on non target organisms such as Daphnia sp, Diplonychus annulatum and Chironomus circumdatus. A qualitative phytochemical analysis was carried out in search of active ingredient and the chemical nature...