Effect on oxidative stress, hepatic chemical metabolizing parameters, and genotoxic damage of mad honey intake in rats (original) (raw)
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Effects of Mad Honey on Some Biochemical Parameters in Rats
Journal of evidence-based complementary & alternative medicine, 2015
The aims of this study were to determine grayanotoxin (GTX-III) toxin level in mad honey using liquid chromatography-tandem mass spectrometry and examine the dynamic changes of certain biochemical parameters in blood serum of rats that consumed mad honey. For the experimental animal study, 20 Sprague-Dawley female rats were divided into 5 groups of 4 rats each, with one group being the control group (Group 1) and the others being the experimental groups (Groups 2-5). Groups 2, 3, 4, and 5 were, respectively, given mad honey extract at doses of 0.3, 0.6, 1.2, and 2.4 mg/g body weight/day via oral gavage for 8 days. According to results, the quantity of GTX-III found in the honey sample as 39.949 ± 0.020 μg GTX-III/g honey, and the biochemical analysis of the tested parameters (aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, alkaline phosphatase, creatine kinase, and creatine kinase muscle and brain) showed a significant elevation with increasing concentra...
Changes in Hepatic Glutathione and Malondialdehyde Levels in Honey Fed Wistar Rats
Biosciences, Biotechnology Research Asia, 2011
Honey is a delicious viscous sweetener made naturally by bees for their own nourishment. Honey comes in a range of colours including white, amber, red, brown and almost black. Honey is a mixture of sugars and other compounds. With respect to carbohydrates, honey is mainly fructose (38.2%) and glucose (31.1%) though others include maltose, sucrose and complex carbohydrates (Anon, 1995). Honey is known to exhibit a broad spectrum of activities including antiviral, antibacterial and immunostimulant (Molan, 2002; Mato, et al., 2003). It was found to have antioxidant activity due to its high content of flavonoid (Mabrouk, et al., 2004). Honey has been found to have a hepatoprotective activity against methyl nitrosoureainduced oxidative stress and inflammatory response in rats by 100% by keeping normal defense system
2011
Honey and ghee are widely used in our diet. Ayurveda emphasized that heated honey and honey mixed with equal amount of ghee p roduce deleterious effects that eventually lead to death. This study reveals their effect o n multicomponent antioxidant system in rat liver . Six groups of rats were maintained for 6 weeks as Control; H oney incorporated diet; H eated honey in corporated diet; G hee incorporated diet; H oney with ghee incorporated diet and h eated honey with heated ghe e incorporated diet . Results revealed a significant rais e in serum alkaline phosphatase, uric acid, hep atic glutathione S - transferase , glucose - 6 - phosphate dehydrogenase and γ - glutamyl transpeptidase with associated increase in serum conjugated dienes , hydroperoxides and malondialdehyde of rats fed with honey mixed ghee and heated honey mixed with heated ghee. Hence, it is concluded that consumption of honey with equal a mount of ghee and its mixture in the heated forms raises the detoxifying enzym...
Liver and Renal Cell Damage Following Excess Bee Honey Consumption in Male Wistar Rat
Biology, Medicine, & Natural Product Chemistry
Honey is a widely used natural product with several health benefits. However, there is paucity of information on its excessive usage. The present study investigated the effect of excess honey consumption on hepato-renal functions in male wistar rats. Twenty-eight adult male Wistar rats were selected into four groups (n=7) and treated with distilled water (control) and 1ml, 2ml and 3ml of honey respectively for 5 weeks. Thereafter, the animals were euthanised and blood as well as kidney and liver were collected for further studies. There was a significant increase in Creatinine, Bilirubin, Urea AST, ALP, total protein as well as a significant decrease in RBC, WBC, haemoglobin, lymphocyte and PCV. Histology of the liver and kidney revealed a significant degeneration and necrosis in a dose dependent manner. This study suggest that excess honey consumption causes liver and renal cellular damage as well as haematological alterations.
2009
The present study was undertaken to investigate the protective effect of Indian honey on acetaminophen induced oxidative stress and liver damage in rat. Honey serves as a source of natural medicine, which is effective to reducing the risk of heart disease, liver toxicity and inflammatory processes. The hepatoprotective activity of the Indian honey was determined by assessing levels of Serum transaminases, ALP and total bilirubin. Finally, the effects of the test substances on the antioxidant enzymes of the liver were also studied by assessing changes in the level of reduced glutathione, glutathione peroxidase, catalase and superoxide dismutase. Serum transaminase, ALP and total bilirubin level were significantly elevated and the antioxidant status in liver such as activities of SOD, CAT, GPx and the levels of GSH were declined significantly in APAP alone treated animals. Pretreatment with honey and silymarin prior to the administration of APAP significantly prevented the increase in the serum levels of hepatic enzyme markers and reduced oxidative stress. The histopathological evaluation of the livers also revealed that honey reduced the incidence of liver lesions induced by APAP. Results suggest that the Indian honey protects liver against oxidative damage and it could be used as an effective hepatoprotector against APAP induced liver damage.
Journal of Medicinal Plants Research, 2011
Honey contains antioxidants such as phenolic compounds that prevent cellular oxidative damage that lead to aging, diseases and death. The aim of this study is to determine the effect of local gelam honey supplementation on oxidative status of young and middle-aged rats. Thirty-six male Sprague-Dawley rats were divided into two age groups; young (2 months) and middle aged (9 months). Each group was further divided into three groups; fed with plain water (control), supplemented with 2.5 and 5.0 g/kg of gelam honey for 30 days. DNA damage level was determined by comet assay, plasma malondialdehyde by high performance liquid chromatography and antioxidant enzymes activities (superoxide dismutase; SOD, glutathione peroxidase; GPx and catalase; CAT) were determined in the erythrocytes and liver. Results showed that supplementation of gelam honey reduced DNA damage, MDA level and GPx activity in the liver of both age groups. But erythrocytes GPx activity in young rats increased markedly wi...
HONEY ENHANCES THE HEPATIC MULTI-FUNCTIONAL DETOXIFICATION PROCESS
Reactive oxygen species-induced cell damage is involved in numerous diseases, including cancer. Reducing oxidative stress is the strategy of chemoprevention via antioxidant-dependent induction of detoxifying enzymes. Honey contains bioactive constituents such as organic acids, trace elements, vitamins, amino acids, proteins and a wide range of polyphenolic phytochemicals. In this study the effect of oral honey solution administration is examined on the activation of the hepatic drug metabolizing enzymes. Oral honey was administered in doses of 2.5 and 5 g/Kg body weight to adult male albino rats for 30 days. Results indicated that the lower dose of honey is more effective, that it increases significantly the activity of all of the three phases. Phase I represented in cytochromeP450 2E1, phase II metabolizing enzymes as epoxide hydrolase-1, glutathione-S-transferase and UDP-glucuronosyltransferase-1 and phase III transporters as nuclear factor erythroid derived 2 like protein.
Effects of honey HMF on enzyme activities and serum biochemical parameters of Wistar rats
Environmental science and pollution research international, 2016
Hydroxymethylfurfural (HMF) is a by-product of thermal degradation of glucose and fructose. In this study, the effects of high HMF content of honey on biochemical parameters of rats were investigated. Experiments were conducted with 40 Wistar albino male rats, each weighing 250-350 g and covered a period of 5 weeks. The animals were divided into five groups. The first group was served as control group. HMF was injected subcutaneously at a dose of 200 mg/kg rat b.w. to the animals in group 2. Group 3 was fed with honey that contains 10 mg HMF/kg honey. In group 4 and 5, there were honeys that contain significantly high HMF content due to long storage period (181 mg HMF/kg honey) and heat process (140 mg HMF/kg honey). At the end of the feeding process, biochemical blood parameters of rats were investigated. It was observed that there were no differences among the glucose, triglyceride, HDL cholesterol, uric acid, Na, GGT, and ALP parameters of the groups. On the other hand, significa...
Anzer honey prevents N-ethylmaleimide-induced liver damage in rats
Experimental and Toxicologic Pathology, 2009
N-ethylmaleimide (NEM) is a sulphydryl blocker which impairs the sulphydryl dependent antioxidant system (mainly glutathione) in the body by alkylating endogenous sulphydryls. This study was designed to investigate the effects of Anzer honey on NEM-induced liver injury in rats. Thirty female Wistar albino rats were divided equally into three groups. Group 1: control; Group 2: NEM; Group 3: Anzer honey+NEM. NEM (0.075 mg kg À1) was given to both group 2 and 3 administered subcutaneously (s.c.) for 30 days. The animals in the Anzer honey+NEM group were treated with Anzer honey at a dose of 0.275 g kg À1 , (p.o.) at 1 h prior to every NEM injection. At the end of the 30 day treatment period, liver samples were taken for determination of the glutathione levels and histological examination. NEM treatment alone caused a significant reduction of the liver glutathione levels in group 2. Furthermore, NEM treatment caused congestion and mononuclear cell infiltration in the liver when compared to the control group. In group 3, Anzer honey treatment reversed all the changes in glutathione level, as well as histopathological alterations, normally induced by NEM. The findings imply that depletion of glutathione concentration plays a causal role in NEM-induced liver injury, and that the hepatoprotective effect of Anzer honey may be mediated through sulfhydrylsensitive processes. They further imply that it may also possess antioxidant properties.
Daily consumption of honey: effects on male Wister albino rats
2012
This study was primarily designed to investigate the effect of acute administration of honey on the biochemical parameters on male Wister albino rats. Fifteen male rats were divide into five groups of three each and were administered with distilled water, 10%, 20%, 50%, and 100% (v/v) of pure honey daily for seven days in that order. On the eighth day the rats were humanly sacrificed and blood samples were collected for analyses. Diastase activity and hydroxylmethylfurfural level were determined from the honey. Serum protein, albumin, globulin, glucose, urea, creatinine, alkaline phosphatase, alanine aminotransferase, total cholesterol, HDL, LDL, VLDL and complete blood counts were determined by automatic analyzer. Diastase activity and hydroxylmethylfurfural level were below the imposed limits. Significant (p < 0.05) increased in body weight was observed as the administration progressed days except on 1st and 2nd days at 20% (v/v). Also within the treatment group, the body weight increased in a concentration dependent manner from 10% to 20% but started decreasing from 50% to 100% (v/v). Significant (p < 0.05) decrease in creatinine level was observed from 20% to 100% (v/v). ALP and ALT activities dropped at 100% (v/v). However, significant (p < 0.05) decrease in triglyceride and VLDL were observed with simultaneous significant (p < 0.05) increase in packed cell volume, mean corpuscular hemoglobin and white blood cell counts at 20% (v/v) treated Int.