Simple Sequence Repeats (SSR) Analysis of Geneticintraspecific Relationships of Moringa Oleiferapopulations from Nigeria (original) (raw)

Moringa oleifera Lam. (Moringaceae) is a multi-purpose economic plant whose commercial demand is on the increase in Africa. To meet this demand, it is significant to step up collections and diversity studies adaptable to higher productivity and utilization. The present study focuses on recently collected landraces/accessions of six populations of M. oleifera from different eco-geographical locations within Nigeria. A total of 70 accessions were evaluated for genetic intraspecific diversity using 20 SSR markers. Among the 20 SSR markers screened, 10 primer pairs (forward and reverse) were selected based on clear amplification products and reproducible scorable bands. Analysis of Molecular Variance (AMOVA), Principal Coordinates Analysis (PCoA) and cluster analysis (CA) were used to evaluate the genetic intraspecific diversity. A total number of 74 alleles with a range of 4 to 15 were detected among the 70 accessions. On the average, 7.4 alleles per locus were amplified in each access...