Simple Sequence Repeats (SSR) Analysis of Geneticintraspecific Relationships of Moringa Oleiferapopulations from Nigeria (original) (raw)
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Genetic diversity in 10 Moringa oleifera Lam. landraces from Western Nigeria was assessed using 10 arbitrary random amplified polymorphic DNA (RAPD) markers. A total of 595 fragments were obtained, out of which 485 (81.5%) were polymorphic. Primer OPT-5 exhibited the least percentage of polymorphism (26.8%) compared to other primers used. Primers OPB-3, OPB-6, OPH-2, OPH-5, OPH-6, OPT-3, and OPT-4 gave 100% polymorphism. The number of amplified fragments per primer ranged from 44 (OPT-2) to 123 (OPT-5) with a polymorphic information content (PIC) ranging from 0.8301 to 0.9280. Unweighted Pair Group with Mean Average (UPGMA) clustering placed the genotypes into two major groups. 'MO2' and 'MO4' showed very close relatedness from the dendrogram obtained while 'MO10' was genetically isolated from the other genotypes. 87% dissimilarity was revealed by the dendrogram. The high degree of polymorphism obtained suggests that RAPD is very useful for genetic diversity studies in M. oleifera.
2012
Genetic diversity in 10 Moringa oleifera Lam. landraces from Western Nigeria was assessed using 10 arbitrary random amplified polymorphic DNA (RAPD) markers. A total of 595 fragments were obtained, out of which 485 (81.5%) were polymorphic. Primer OPT-5 exhibited the least percentage of polymorphism (26.8%) compared to other primers used. Primers OPB-3, OPB-6, OPH-2, OPH-5, OPH-6, OPT-3, and OPT-4 gave 100% polymorphism. The number of amplified fragments per primer ranged from 44 (OPT-2) to 123 (OPT-5) with a polymorphic information content (PIC) ranging from 0.8301 to 0.9280. Unweighted Pair Group with Mean Average (UPGMA) clustering placed the genotypes into two major groups. ‘MO2’ and ‘MO4’ showed very close relatedness from the dendrogram obtained while ‘MO10’ was genetically isolated from the other genotypes. 87% dissimilarity was revealed by the dendrogram. The high degree of polymorphism obtained suggests that RAPD is very useful for genetic diversity studies in M. oleifera. ...
Genetic diversity and population structure of Moringa oleifera
Conservation Genetics, 2013
Moringa is a genus of the tropical flowering plant family Moringaceae containing 13 diverse species. Among the different species, only Moringa oleifera L. is cultivated. This species has great potential in serving as a high-value crop for food, medicinal products, as well as fodder for animals, particularly in developing tropical regions of the world. In this study, the genetic diversity and population structure of world-wide collections of M. oleifera were investigated using DNA markers. A total of 19 microsatellite or simple sequence repeat (SSR) markers along with a partial sequence of the chloroplast gene atpB were used to study genetic diversity within 161 accessions of M. oleifera collected from Asia, Africa, North and South America, and the Caribbean. On average, 8.3 alleles/per SSR were amplified in each accession. A total number of 158 alleles were detected in 131 accessions collected from the wild in Pakistan and from 30 accessions obtained from ECHO (Florida). Observed he...
Evaluation of agronomic practices, genetic diversity and population structure of Moringa oleifera (Lam.) is crucial to its sustainable utilization to ensure food and nutritional security. Four agronomic practices of field preparation and soil analysis, regeneration, weeding and pruning, as well as harvesting, were adopted. Genetic diversity and population structure of 20 populations were evaluated using amplified fragment length polymorphism (AFLP) primer pairs (E-ACC/M-CAC and E-ACA/M-CAG). The effects of agronomic practices on leaf quantity production were evaluated using standard procedures. The physico-chemical and morphological data were analyzed using descriptive statistics, while genetic diversity, population structure, dendrogram reconstruction, and Principal Component Analysis (PCA) were analyzed from the AFLP genetic data. Significant effects (P ≤ 0.05) of agronomic practices on the quantity of leaf production were recorded across the accessions. The two primer pairs gener...
Journal of Medicinal Plants and By-products (JMPB), 2019
The present study was conducted to investigate genetic diversity between and within of six populations with different individual numbers of cultivated and non-cultivated provenances of Moringa peregrina (Forssk.) Fiori and Moringa oleifera Lam. using RAPD, ISSR and various combinations of RAPD and ISSR primers in one multiplex PCR (R-ISSR). 10 primers that produced clear and reproducible fragments after screening of 30 primers were selected for further analysis. A set of 10 primers generated 96 bands ranging in size from 150 to 1600 bp, corresponding to an average of 16 bands per primer and out of which 100 % were polymorphic among 26 individuals. The PIC values ranged from 0.16 to 0.31 and MI values ranged from 2.16 to 4.65 per primer. The primer R-ISSR (H876+A17) had the highest PIC (0.31) and MI (4.65) values. A maximum and minimum genetic similarity values were observed between populations (I and V) in M. oleifera (0.98) and populations (III and IV) in M. peregrina (0.52) respectively. The Gst value was 0.7, indicating that 61% of the genetic diversity resided within the populations. Clustering analysis using average algorithm based on Nei's unbiased genetic distance, classified the Moringa Adans. populations into five major groups. The PCOA data confirmed the results of clustering. The results of this study revealed that R-ISSR markers could be efficiently used for genetic differentiation of the Moringa individuals. The primers used in this article are useful to detection of a high level of polymorphism and it can be used to guide future breeding studies and management of Moringa genus.
Genetic variability, correlation and path analysis in Moringa (Moringa oleifera L.)
2018
Analysis of variance indicated that there is an existence of significant variability among the twenty accessions for all the characters under study. The highest GCV was recorded for pod weight, number of leaves per rachis, yield per plant. Heritability estimates were high for pod weight, yield per plant, number of pods per plant, length of pod, and number of leaves per rachis. The expected genetic advance as a percentage of mean was high for pod weight, number of leaves per rachis, yield per plant, length of pod, number of seed per pod. Yield per plant had significant and positive association with stem girth fruit setting percentage length of pod girth pod weight and number of pods per plant indicating their usefulness in selection for yield. Non-significant and negative association with plant height leaf length number of leaves per rachis length of leaf rachis number of flower per inflorescence number of seeds per pod. Among the twelve characters studied, six characters viz., by pl...
Phenotypic Intraspecific Variability among some accessions of Moringa oleifera (Lam)
Phenotypic characterization of plant species is the basis for selection, conservation as well as improvement. Moringa oleifera (Lam.) is commonly grown as multi-purpose medicinal and leafy vegetable crop in Nigeria. This study evaluated phenotypic intraspecific variations among 40 accessions of M. oleifera collected from different agro-ecological zones. A total of 30 morphometric traits involving qualitative and quantitative vegetative, floral, fruit/pod and seed traits, seed set and germination percentages were combined for the analyses. Descriptive statistics, variance analysis, correlation coefficient, principal component analysis (PCA) and cluster analysis (CA) were used to evaluate the intraspecific variability. The accessions showed marked differences in floral, fruit pod and seed characters analyzed. Accessions edN037 (71 days), osN024 (73 days), ogN028 (74 days), oyN003 (75 days) and abN059 (76 days) were identified as early maturing accessions. Correlation coefficients r ≥ 0.70 were high and significant for reproductive characters. The PCA and CA generated similar results. The first five principal component axes explained 61.40% of the total variation with PC1 (23.92%) and PC2 (14.19%) contributing 38.11% of the total variation. The CA showed that the degree of intraspecific similarity was high (66.82%) based on Euclidean similarity index. Nevertheless, four clusters were formed indicating intraspecific phenotypic dissimilarity among the 40 accessions especially with the separation of accessions that were collected from similar environments. The phenotypic variations could be explored for utilization, conservation and for future genetic improvement by selection of accessions with promising agronomic characters.