Effect of Bile Salts on Gastric Emptying and Intestinal Transit in the Rat (original) (raw)

Although much work has been done on the effects of bile salts on gastric and intestinal motility,l-7 there have been few reports of the effects of bile salts on gastric emptying and intestinal transit. 6 ,7 Studies, presently being conducted in our laboratory on the effect of orally administered bile salts on drug absorption, have led to a consideration of the influence of bile salts on gastric emptying and intestinal transit rates. The absorption of many drugs depends upon their presence in specific areas of the gastrointestinal tract. An increase or delay in gastric emptying or intestina l transit could markedly affect the absorption characteristics of these drugs. The following study was designed to investigate these areas. Materials and Methods 1Vlaterials. Sodium taurodeoxycholate was obtained from Maybridge Chemical Company, Ltd., North Cornwall, United Kingdom. It was chromatographically pure. Sodium deoxycholate (special enzyme grade) was obtained from Mann Research Laboratories, Inc., New York, New York. Methods. Male Sprague-Dawley descent rats (Blue Spruce Farms, Altamont, New York) weighing between 130 and 180 g were fasted for 24 hr prior to the experiment. Water was allowed ad libitum. One and one-half milliliters of a 0.07% phenol red solution (prewarmed to 35 to 37 C),