Use of Salivary Alpha Amylase to Evaluate Dental Stress in Children during their First and Subsequent Visits: A Pilot Study (original) (raw)
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Levels of Anxiety and Salivary Alpha-Amylase in Children During Restorative Dental Treatment
Pesquisa Brasileira em Odontopediatria e Clínica Integrada, 2016
Objective: To assess levels of anxiety and salivary alpha-amylase in children and its association with the use of informative or aversive behavioral management techniques during restorative dental procedures. Material and Methods: A cross-sectional study was performed with 69 children aged 4-12 years submitted to restorative dental treatment. The level of anxiety of patients was verified by means of the Modified Venham Picture Test and levels of salivary alpha-amylase were measured before and after the restorative procedure. The behavioral management techniques used during the dental procedure were registered. Associations between variables were analyzed using the chi-square test, considering statistically significant associations with p ≤ 0.05. Results: Salivary alpha-amylase showed high and moderate levels prior to dental procedure in 47 children (68.1%). Variables gender and behavioral management techniques were not associated with anxiety, as measured by VPT and salivary alphaamylase activity. However, younger children showed higher levels of alpha-amylase (p = 0.001). Conclusion: The sample revealed that the visit to the dentist has generated anxiety, manifested by behavioral and physiological changes, especially in children under 72 months; thus, no association between anxiety and the different management techniques was observed.
A Salivary α-Amylase Level in Relation to the Oral Health Parameters among Children in Baghdad City
Journal of Baghdad College of Dentistry, 2016
Background: Saliva is a specific bio-fluid with important biomarkers. Analyzing any alternation in these markers could give valuable information, in relation to oral health status parameters. The aim of this study was to investigate the level of α-amylase in unstimulated whole saliva of healthy, primary school children in relation to some oral health parameters. Materials and Methods: A questionnaires consisted of demography and medical histories of participants were filled by children families. Saliva samples were collected for 5-minutes between 9:00-11:00 AM from 114 healthy students aged 6-13 years, divided into four age groups. Flow-rate, Plaque and Gingival Index were assessed and dentition status was investigated by DMFT/dmft using WHO criteria. Salivary amylase was analyzed in unite per litter, using quantitative colorimetric amylase determination at 585nm. Results: A significant positive correlation was found between age and salivary flow-rate, (r=0.362, P < 0.001). Salivary α-amylase concentration increased significantly with age (P< 0.001). For each one year there is an increase in age, amylase level is expected to increase by 5.2 U/L. A male gender is expected to reduce salivary α-amylase level by 10.6 U/L compared to female, however the effect was not significant. Gingival index was positively, although nonsignificantly associated with salivary α-amylase concentration. DMFT showed a significant weak positive linear correlation with salivary amylase level (r=0.309, P<0.001), while deciduous teeth decay experience and plaque index were significantly and negatively associated with salivary amylase. Conclusion: Results emphasize the importance of salivary amylase, as a non-invasive biomarker in regulating oral and dental health status in children.
Journal of Clinical Pediatric Dentistry, 2017
Objectives: this study was aimed at comparing dental stress in children having their first dental visit to those returning for dental treatment using salivary biomarkers of stress including salivary cortisol (s-cortisol), Immunoglobulin-A (s-IgA) and alpha-amylase (s-α-amylase). Additionally, the study was aimed at monitoring the change in stress in new patients as they progressed from the waiting to the clinical areas. Study design: salivary samples were collected from 40 children who had not been to a dentist before and similar samples were collected from 40 children who were returning for completion of dental treatment. Salivary cortisol, s-IgA and s-α-amylase concentrations were obtained by Enzyme-linked Immunosorbent Assay (ELISA). Results: salivary cortisol levels were higher for new patients at the waiting area compared to that at the dental chair (p=0.05). Salivary alpha-amylase significantly increased in new patients while being seated in the dental chair. Returning patient...
Revista de Chimie, 2018
Human saliva is a complex mixture of fluids considered to have a real potential in maintaining the dental health, carrying out important functions in the local modulation of inflammatory reactions and immune response. The biologic properties of salivary alpha-amylase are a subject of intense debate in the scientific literature, regarding its use as a biomarker for local and systemic diseases, including caries lesions. The purpose of our study was to measure the correlation between the salivary level of alpha-amylase and the incidence of caries in a group of children with young permanent teeth. A total of 128 participants were included in four study groups according to presence or absence of dental caries, based on specific inclusion/exclusion criteria and a scoring system between 0-5, depending on gender and the number of caries lesions. From each patient, 10 mL of saliva was collected and examined with a spectrophotometer in order to determine the level of salivary alpha-amylase. T...
Effect of alpha amylase on early childhood caries: a matched case-control study
Brazilian Dental Science, 2013
Objectives: There are a few studies addressing the relationship between salivary alpha-amylase and dental caries. This study was implemented in order to investigate the effect of salivary alpha-amylase level on early childhood caries (ECC).Materials and Methods: In this matched case-control study, which was carried out from November 2011 to March 2012 in Hamadan City, the west of Iran, mean levels of salivary alpha-amylase of 84 ECC-active cases were compared to that of 84 ECC-free controls using spectrophotometric method to assay enzyme kinetics. The two groups were matched by oral hygiene (frequency of tooth brushing per day) and food habits (frequency of sugar consumption between servings). Subsequently, a subsample of 28 cases was undergone emergency treatment of dental caries. Alpha amylase level was measured before treatment and 30 to 45 minutes later.Results: The results indicated an inverse relationship between alpha-amylase level and dental caries. The mean level of salivar...
Salivary Alpha-Amylase Activity: A Possible Indicator of Pain-Induced Stress in Orthodontic Patients
Pain Medicine, 2011
Introduction. Pain, a common experience reported by orthodontic patients, has its intensity assessed with the help of subjective scales, which have a limited and disputable value. Such unpleasant experience, which may raise stress levels, is reflected by an increase in the salivary concentration of alpha-amylase. Objective. Assess the correlation between the salivary levels of alpha-amylase and pain intensity reported by patients during orthodontic treatment. Patients. Twenty male patients (11-37 years of age) were assessed daily, before treatment, after bracket bonding, and after initial arch wire insertion. Design. Saliva was sampled for alpha-amylase analysis, and pain intensity was measured with the visual analog scale. Results. There was no correlation between alphaamylase concentrations in the saliva and pain intensity, although the patients had a significant and progressive increase of alpha-amylase levels during the assessment period. Conclusions. The findings may reflect the psychological stress caused by the presence and activation of the fixed appliance.
International Journal of Applied Pharmaceutics, 2018
Objective: Visual and hearing impairments are known to profoundly affect children’s psychological development, including their anxiety. However, strong emotional relationship with their mother found in these children may help them cope. Measuring salivary alpha-amylase (sAA) in saliva samples is widely used in clinical settings as a reliable, non-invasive biomarker of anxiety level. Methods: Our objective was to evaluate sAA levels in visually and hearing-impaired children and their mothers before and after dental treatment. This study included 60 children with visual and hearing impairments and their mothers. sAA level of both children and mothers was sampled while they were together in the waiting room before treatment. The children then underwent dental prophylaxis in a separate room, and their post-treatment sAA was measured immediately afterward. At that time, their mother’s post-treatment sAA was sampled in the waiting room. Data were analyzed using a Wilcoxon test. Results: s...
European journal of paediatric dentistry : official journal of European Academy of Paediatric Dentistry, 2013
Few studies have examined psychological stress and personal anxiety in children exhibiting cooperative behaviour during dental treatment. We assessed psychological stress and personal anxiety during dental treatment in cooperative children, and investigated the influence of various factors. We measured pre- and post-treatment salivary alpha amylase (sAA) levels of 28 children aged 8-13 years and their parents. Children completed the State-Trait Anxiety Inventory for Children (STAIC); their parents completed the STAI. The IA group included children whose sAA levels increased >10%, whereas the DA group included children whose sAA levels decreased >10%. We used regression models to calculate the power of variables to predict children's psychological stress. The mean anxiety trait score in the IA group was significantly higher than in the DA group (t-test, P = 0.021). For children with higher STAIC-Trait scores, the OR for increasing sAA was 1.16 (95% CI [1.02-1.31]). Parental...
The Effect of Dental Treatments in Caries Management on Stress and Salivary Protein Levels
Journal of Clinical Medicine
A great burden is put on healthcare systems by dental caries and understanding patients’ treatment needs is of utmost importance. The aim of this pre–post study was to assess dental anxiety and the psychological stress induced by two different types of dental treatment (prophylaxis and cavity preparation), by combining psychometric evaluations with salivary biomarkers, in a group of 28 schoolchildren presenting in a university clinic. Pre- and post-treatment unstimulated whole saliva was collected and levels of cortisol, alpha-amylase (sAA) and total protein content were measured. The State–Trait Anxiety Inventory for Children and the Frankl Behaviour Rating Scale (FBRS) were applied. Statistical analysis was performed using the Stata/IC 16 (StataCorp) programme. All salivary parameters showed strong positive correlations between pre- and post-treatment levels. Post-treatment, salivary cortisol decreased (p = 0.008, paired t-test), sAA did not change significantly (p = 0.572, sign t...
Experimed
Objective: Anxiety due to the dentist and dental treatment is a problem encountered in many children. The aim of the present study is to determine salivary nitric oxide, lactoferrin, α-amylase and cortisol levels of children in primary and mixed dentition, and to evaluate their relation with stress due to dental treatment. Material and Method: The study consisted of 50 children in primary and mixed dentition. The children were evaluated clinically and according to Frankl Behavior Rating Scale. Salivary flow rate was calculated, and nitric oxide, lactoferrin, α-amylase and cortisol levels were measured in saliva. Results: 68% percent of the children were found to be negative according to the Frankl Behavior Rating Scale (category 2), and significantly decreased salivary flow rate was evident in these children when compared with children that were categorized as completely negative (category 1). The DMFT+dft index was 7.56±4.29, and positive correlations were found between DMFT+dft indices and salivary nitric oxide, lactoferrin, cortisol and α-amylase levels (p<0.05). These parameters were not different between genders. Positive correlations were found between salivary nitric oxide and α amilase, cortisol and α amilase, cortisol and lactoferrin; and also between α-amilase and lactoferrin levels (p<0.05). Conclusion: Salivary lactoferrin, α-amylase and cortisol may be suggested as important parameters of oral health.