ANTIOXIDANT ACTIVITY AND BIOLOGICAL EVALUATIONS OF PROBIOTIC BACTERIA STRAINS (original) (raw)
The antioxidant activity of four probiotics strains belongs to the genera Lactobacillus (two strains), Bifidobacterium as well as Probionebacterium were investigated through the DPPH • and ABTS •+ scavenging ability of the cell free extract of bacteria of the probiotic strains comparing with the standard antioxidant ascorbic acid and BHT. The results of the DPPH scavenging potential of cell free extract showed that the maximum antioxidant activity with Probionebacterium freudenreichii ( 97.75 % ) and it was significant increased compared with vit.c and BHT followed by Lactobacillus retueria (96.74 % ) activity. All cell free extracts showed highly scavenging potential against ABTS radical. The present study evaluated the survival of free probiotic bacteria under acidic conditions (pH 2 and pH3) during 3h, L.GG showed intolerance to pH2, but showed more acid tolerance at pH3. In contrast, the other strains showed more acid tolerance at pH values where incubation of strains for 3h at pH2 resulted in a decrease of about one log cycle, while at pH3 the decrease ranged from 0.2 to 0.7 log cycles. A similar decrease in cell viability was observed in the case of 1% and 2% bile salt for L.reuteri and B.breve, the decrease ranged from 0.4 to 0.6 log cycles, while in case of L.GG and P. Jensenii the decrease in cell viability was about 1.5 log cycle. However, free cells were survived at 2 % bile salt. The biological evaluations of biscuit incorporated with four encapsulated probiotic bacteria strains and biscuit without encapsulated probiotic bacteria were studied in albino rats. The results showed that non significant change in (, Total cholesterol and HDL-C) but there were significant change in ( serum triglycerides and LDL-C ). The safety of experimental diet was studied through serum liver and kidney functions where non significant change in liver function (serum alanine aminotransferase, aspartate aminotransferas and alkaline phosphatase). Also non significant change in kidney function (Creatinine & Urea).
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