Physico-Chemical and Melissopalynological Characterization of Czech Honey (original) (raw)
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Physicochemical characterization of natural honeys from different regions in Slovakia
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 2014
This study is intended to determine the physicochemical characteristics of Slovakian honeys, and compare them with specifi cations described in the Codex Alimentarius Standard, the European Honey directive, the Slovak Codex Alimentarius and Slovak standard No. 1/2006. In addition, we tried to fi nd out correlations between individual constituents of honeys. Physicochemical characterization was carried out following the harmonized methods dictated by the International Honey Commission IHC. Honey samples (n=50) were collected from three honey types (blossom, blends, honeydew), and from the three main Slovakian geographical regions (named 'east', 'middle' and 'west'). The physi co che mi cal evaluation included moisture, reduced sugars, proline, hydroxymethylfurfural (HMF), conductivity, diastase and invertase activity, pH and water activity, following the techniques proposed by the European Honey Commission (EHC). The moisture content in the honey varied from 15.26 to 17.65 %, HMF levels ranged from 21.83 to 63.00 mg. kg −1 , the diastase activity varied from 21.01 DN to 36.67, invertase activity varied from 121.73 to 164.11 U. kg −1 , the proline content varied from 426.56 to 531.79 mg. kg −1 , the fructose content values were found from 36.33 to 40.20 g. 100g −1 , the glucose content values ranged from 27.67 to 31.00 g. 100g −1 , the values of saccharose content were from 0.15 to 0.37 g. 100g −1 , the conductivity varied from 29.48 to 97.24 mS. cm −1 , the pH value varied from 4.06 to 4.80 and the water activity content varied from 0.55 to 0.57. Signifi cant diff erences (P ≤ 0.05) were found between HMF and fructose, glucose and saccharose and conductivity and pH, signifi cant differences (P ≤ 0.01) between fructose and glucose and signifi cant diff erences (P ≤ 0.001) were found between HMF and conductivity. Between blossom honey and honeydew honey were found statistical signifi cant diff erences in HMF (P ≤ 0.05) and conductivity between honeydew honey and blossom honey (P ≤ 0.001). Among regions were determined statistical diff erences between HMF and invertase (P ≤ 0.05) and conductivity (P ≤ 0.01).
Characterisation and Classification of Croatian Honey by Physicochemical Parameters
Food Technology and Biotechnology, 2008
The aim of this study is to characterise 8 different monofloral and multifloral types of Croatian honey (a total of 254 samples from 2003, 2004, and 2005 harvesting seasons) based on 11 common physicochemical parameters (water mass fraction, total reducing sugar mass fraction, sucrose mass fraction, ash mass fraction, electrical conductivity, acidity, diastase and invertase activity, hydroxymethylfurfural (HMF) mass fraction, proline mass fraction and optical rotation). Differences in the above-mentioned parameters, established among the honey samples, are influenced by different factors, such as botanical origin, climate and regional circumstances. After the sample characterisation, results obtained for 2 monofloral (acacia (Robinia pseudoacacia) and chestnut (Aesculus hippocastanum)), and 2 multifloral (floral and meadow) honey types were subjected to the pattern recognition procedures. In this regard, unsupervised methods such as cluster and principal component analyses were employed, with the goal of evaluating the possibility of differentiation of Croatian honey stemming from different botanical origins, based on their physicochemical profile. Cluster analysis (CA) revealed the existence of two clusters, in the first of which is acacia honey as the best grouped, and the second corresponds to the dispersed group constituted of the remaining three honey types under investigation (chestnut, floral, and meadow). Principal component analysis (PCA), i.e. its first two components, stood for the average of 50.5 % of the data variance. PCA and CA showed that physicochemical parameters are able to provide enough information to allow for the classification and distinction of the types of honey originating from four botanical origins under investigation (acacia, chestnut, floral and meadow).
Physicochemical and melissopalynological characterization of Estonian summer honeys
Procedia Food Science, 2011
The samples of 14 honeys, retained from Estonian beekeepers, were analyzed for parameters such as pH, moisture content, free acidity, electrical conductivity, diastase activity, hydroxymethylfurfural (HMF) content and mineral content, including sodium (Na), potassium (K), magnesium (Mg) and calcium (Ca). Fructose, glucose and disaccharide content were also identified and fructose/glucose ratio was calculated. In addition melissopalynological analyses were carried out for characterization of honeys. The mean values of analyzed honeys were: pH 3.8; moisture 17.3%; free acidity 20.4 mmol/kg; electrical conductivity 0.2 mS/cm; diastase activity 23.1 DN and HMF was below 3.8 mg/kg. Within the mineral content, potassium was quantitatively the most important mineral in the range of 125.79 to 1381.53 mg/kg followed by calcium of 20.37-63.65 mg/kg, magnesium 5.53-25.49 mg/kg and sodium 4.77-19.44 mg/kg. The predominant sugar in honey samples was fructose having the mean value of 35.91 g/100g followed by glucose 35.00 g/100g. The disaccharide average content was 6.00 g/100g. The melissopalynological analyzes showed that the most dominant pollens in honey samples were cruciferous (Cruciferae)-mainly rape (Brassica napus); rosacean (Rosaceae)-mainly raspberry (Rubus idaeus); white clover (Trifolium repens); sweet clover (Melilotus officinalis) and willow (Salix). The results of honey pollen profile analysis and calculated fructose/glucose ratios (0.89-1.17) both indicated to flower honeys. All of the analyzed honeys were found to meet European Legislation (EC Directive 2001/110) for all parameters.
Determination of type of honey produced in the different climatic regions of Bosnia and Herzegovina
International Journal of Environmental Science and Technology, 2016
The aim of this study was to investigate the impact of the environmental characteristics of a particular climate areas of Bosnia and Herzegovina on the type of honey produced in those areas. Physical, chemical and sensory characteristics of honey and pollen content in honey are the subject of ongoing research in the world, but still there is no universal method for classification of honey. Pollen analysis of honey in combination with other tests, e.g., pH, electrical conductivity and sensory properties, is often used for determination of type of honey. Acceptability of honey by consumers is based on the evaluation of sensory characteristics in the first place, which depend on the origin of plants from which the nectar (pollen) or honeydew was collected. Consumers and manufacturers are interested in classification of honeys because the most important characteristics of honey are dependent on its botanical and geographical origin. In this study were examined sensory properties, pH, electrical conductivity and pollen composition of honey samples collected from different climate regions, at different altitudes and at different vegetation season of Bosnia and Herzegovina. Laboratory analyses were carried out in three parallel determinations of the same sample of honey. In this research, 48 samples of honey were examined, out of which 29 were nectar (pollen) honey, 11 were mixed honey and 8 were honeydew.
Applicability of physico-chemical parameters of honey for identification of the botanical origin
Acta Scientarum Polonorum - Technologia Alimentaria
Efforts are being made to apply physicochemical parameters analysis in the identification of varietal honeys. With many variables describing a given population, it is feasible to differentiate between basing on principal component analysis (PCA). The aim of this study was to investigate selected physicochemical quality characteristics of nectar honey, with particular emphasis paid to carbohydrate composition, and to determine its applicability in identifying the variety of floral honey. The experimental materials were samples of commercial honey available at retail in Krakow in 2005-2007 period. The following analyses were performed: water content by the refractometric method, sugars content according to Luff-Schoorl, content of sugars using HPLC, electrical conductivity, specific rotation, and acidity of honey. Application of HPLC allowed the precise qualitative identification of sugars, which was impossible to be determined by Luff-Schoorl method. The obtained results were analyse...
Physico-Chemical Profile of Four Types of Honey from the South of the Republic of Moldova
Food and Nutrition Sciences, 2021
There are many studies that compare the quality and biological characteristics of honey with distinct geographical and botanical origins. However, the physico-chemical and biological properties of different types of honey in the same production regions are rarely mentioned. The honey used in this study: sunflower honey, rapeseed honey, manna honey and polyflora honey, came from GT "Malai C" in Taraclia village, Causeni district in the southern part of the Republic of Moldova and belonged to the flowering season of year 2020. Following the polynecological analysis, it was found that two types of honey are monofloral with a dominant pollen content of Helianthus spp. (49.15%-93.12%) in sunflower honey and Brassica spp. (52.17%-70.11%) in rapeseed honey. Mana honey and polyflora contain several types of pollen. Thus, four types were identified in manna honey, including: Acer platanoides (29.11%-30.11%), Quercus robur (28.67%-29.99%), Rubus idaeus (21.55%-28.78%), Taraxacum officinale (22.21%-28.76%). Polyflora honey contains: Helianthus annuus (24.91%-31.11%), Brassica napus (23.45%-29.18%), Tilia (28.95%-31.92%). Based on a Pfund scale, it was found that the color of the honey varied from a lighter shade for rapeseed honey (water amber 7.66 ± 3.002 mm) to a darker color for sunflower honey and polyflora (extra light amber 34.366 ± 21.01 mm and 36.04 ± 1.115 mm respectively). Spectrophotometric determination of phenolic compounds in honey samples showed that their content ranged from 38.18 mg GAE/kg honey for rapeseed honey to 831.09 mg GAE/kg honey for manna honey. At the same time, the flavonoid content ranged from 28.41 mg QUE/kg honey for rapeseed honey to 151.72 mg QUE/kg honey for manna honey. Mana honey showed a better antioxidant activity than the other honey samples in the study (72.03%). The reported results suggest that manna honey has the best potential and its consumption in the human diet as food with valuable biological properties can be How to cite this paper: Chirsanova, A.,
PHYSICO-CHEMICAL AND TEXTURA L PROPERTIES OF HONEYS FROM NORTH EAST PART OF ROMANIA
The aim of this study is to evaluate the physico-chemical (pH, aw, free acidity, Brix concentration, moisture content, ash content, colour parameters (L*, a*, b*, chroma, hue angle and yellow index, glucose, fructose and sucrose content) and textural (hardness, viscosity, adhesion, cohesiveness, springiness, gumminess and chewiness)) properties of 5 samples of honey of different floral origins (acacia, tilia, sunflower, honeydew and polyfloral). The physico-chemical parameters of honey were in agreement with those reported by other scientists. The moisture content of all the five samples do not exceeded the maximum allowable level of 20% established by the European Commission. The acacia and tilia honeys presented green components, while all the honey presented yellow components. The highest yellow index has observed in the case of tilia honey. The data were submitted to principal component analysis (PCA) and it was found that the two principal components (PCs) explained 100% of the variations in the data set. The PC1 explains 99% of the variability and the PC2 explains 1%. The PC1 separates the honey into two groups: one group below the PC1 represented by acacia, sunflower and polyfloral honeys and the second group below the PC1 represented by tilia and honeydew honeys. Mircea OROIAN, Elena TODOSI SĂNDULEAC, Se rgiu PĂDUREȚ , Physicochemical and t extural properti es of honeys from north east part of Romania, Food and Envir on ment Safet y, Volu me XV, Issue 3 -2016, pag. 234 -239
Analysis of organic molecules, physicochemical parameters, and pollen as indicators for authenticity, botanical origin, type and quality of honey samples examined, 2020
To contribute to Chilean honey’s characterization, 12 honey samples were analyzed using comprehensive physicochemical and pollen analyses. Beekeepers donated samples from La Pintana, Linderos, Cajón del Maipo, and Chiloé. Physicochemical parameters required for honey authentication such as free acidity (in range of 9.5–46 meq/kg), hydroxymethylfurfural (0–8 mg/kg), humidity (14.4–16.9%), sugar profile, amino acid profile, organic acid profile, pH (3.8–4.7), electrical conductivity (0.25–1.47 mS/cm) and diastase activity (28.6–43.8 ºG), were determined by conventional techniques and nuclear magnetic resonance (NMR) at an international quality control laboratory for honey analyses. The Chilean honey samples analyzed showed physicochemical properties in normal ranges and typical sugar profiles for natural honey, which confirmed their authenticity and high quality.
Senzorial and Physico-Chemical Characterisation of Honey from Muntenia Zone, Romania
Current Trends in Natural Sciences, 2018
With a high nutritive value and healing properties, honey is a well known natural food product. Factors like floral source and climate condition influence the properties of honey. The study investigated sensorial and physicochemical characteristics of some honey samples collected directly from the beekeepers from Muntenia Zone, Romania. The colour, pH, moisture content, electrical conductivity, ash content, acidity and total sugar were evaluated. Moreover, these characteristics were compared with the International Standard of Codex Alimentarius. The results showed that all the honey samples had similar physico-chemical properties and respect the limits imposed by EU standards; in this way was proved that the honey from Muntenia Zone is a good quality honey. This standard could be sustained by regular training of local bee farmers in honey harvesting and storage.
Journal of Apicultural Research, 2020
The definition of a variety of natural bee honey is based on a melissopalynological analysis of the pollen grains that are the most prevalent and naming the variety of honey according to the botanical name of the plant. The purpose of this work was to compare the declarations of beekeepers regarding a variety of bee honey from Poland with the results of laboratory analysis. The study was conducted on 100 samples of bee honey. It was shown that 48% of the samples were incorrectly classified in the declared variety. Only heather honey samples showed 100% correct classification. In contrast, 7% of rape honey, 13% of buckwheat, 58% of multifloral, 68% of linden, 100% of dandelion and 100% of willow honey were wrongly named. The consumer, when looking for a specific variety of bee honey, expects health benefits, which is why individual parameters defining the quality of bee honeys, especially varieties, should be the subject of detailed and continuous monitoring.