Continuous wave optical scanner for small animal molecular imaging (original) (raw)

Spectroscopy and irradiation in the visible Ultraviolet (UVvis) to Near Infrared (NIR) ranges are important tools in the most recent research on biological tissues (Banavath, H.N., et al., 2018, pp. 35- 40). On the one hand, the optical properties of tissues depend on the energy of the photons and, according to the spectral range and the irradiation dose, the application of light energy on biological tissues can lead to different applications both in the biological and health sciences (Hopkins, S.L., et al., 2016, pp. 644-653). To carry out experiments that allow to study the light-matter interaction, a flexible experimental set-up was carried out to characterize the usable light sources and also to perform Reflectance measurements (collimated) as well as Fluorescence studies in biological tissues using halogen light sources, LASER (405nm, 532nm and 650nm) and LED light emitting diodes (380nm, 395nm, 518nm, 590nm). m and 640nm). The experimental set-up uses easily available lasers and an optical system that allows preparing the light beam and focusing it on the sample under study. The phenomenological response is measured with a Silicon cell photometer and the spectral measurements are made with mini spectrometers in fiber optic assemblies. Keywords: Biophotonics, LASER, LED, Spectroscopy, Biological tissues.