Diffuse reflectance patterns in cervical spectroscopy (original) (raw)
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Fluorescence and Reflectance Monitoring of Human Cervical Tissue in Vivo a Case Study
2003
An imaging spectrograph, designed and built by Science and Technology International (STI), and a point monitoring system, developed at the Lund Institute of Technology, have been used to measure the fluorescence and reflectance of cervical tissue in vivo. The instruments have been employed in a clinical trial in Vilnius, Lithuania, where 111 patients were examined. Patients were initially screened by Pap smear, examined by colposcopy and a tissue sampling procedure was performed. Detailed histopathological assessments were performed on the biopsies, and these assessments were correlated with spectra and images. The results of the spectroscopic investigations are illustrated by a thorough discussion of a case study for one of the patients, suggesting that the techniques are useful in the management of cervical malignancies.
Gynecologic Oncology, 1997
ples. Our findings, and those of others , raised the possibil-Fourier-transform infrared (FT-IR) spectra of malignant and ity that FT-IR spectroscopy may have a potential as a diagdysplastic cervical scrapings were abnormal, as first described in nostic method. our study of a limited number of samples, where the spectra were Our initial study , aimed at the evaluation of structural evaluated by visual inspection and peak intensity ratios. We have changes associated with cervical carcinogenesis, was based expanded our study to evaluate more cervical conditions, and to on a limited number of samples. Moreover, discrimination analyze the spectra by a chemometric approach (principal compobetween spectra was achieved by visual inspection and comnent analysis [PCA]). Cervical samples from 436 females were parison of peak intensity ratios. However, visual inspection evaluated by FT-IR and Papanicolaou testing; 40/436 spectra is not the ideal method to compare spectra. This approach were nonanalyzable. The remaining were as follows: normal, 174; lends itself to subjective bias and provides no quantitative malignant, 19; dysplasia, 8; atypia, 113; atrophy, 19; inflammatory,
In-vivo fluorescence and reflectance imaging of human cervical tissue
SPIE Proceedings, 2003
A hyperspectral imaging spectrograph has been used to measure the fluorescence and reflectance of cervical tissue in vivo. The instrument was employed in a clinical trial in Vilnius, Lithuania, where 111 patients were examined. The patients were initially screened by Pap smear, examined by colposcopy and a tissue sampling procedure was performed. Detailed histopathological assessments were performed on the biopsies, and these assessments were correlated with spectra and images. The results of the spectroscopic investigations show that different tissue types within one biopsy region exhibit different spectral signatures. A spectral analysis of the entire image localizes dysplastic regions in both fluorescence and reflectance, suggesting that the hyperspectral imaging technique is useful in the management of cervical malignancies.
Fluorescence and reflectance monitoring of human cervical tissue in vivo: a case study
Spectral Imaging: Instrumentation, Applications, and Analysis II, 2003
An imaging spectrograph, designed and built by Science and Technology International (STI), and a point monitoring system, developed at the Lund Institute of Technology, have been used to measure the fluorescence and reflectance of cervical tissue in vivo. The instruments have been employed in a clinical trial in Vilnius, Lithuania, where 111 patients were examined. Patients were initially screened by Pap smear, examined by colposcopy and a tissue sampling procedure was performed. Detailed histopathological assessments were performed on the biopsies, and these assessments were correlated with spectra and images. The results of the spectroscopic investigations are illustrated by a thorough discussion of a case study for one of the patients, suggesting that the techniques are useful in the management of cervical malignancies.
Optical diagnosis of cervical cancer by fluorescence spectroscopy technique
International Journal of Cancer, 2006
In the present work, we examine normal and malignant stage IIIB cervical tissue by laser induced fluorescence, with 2 different objectives. (i) Development of the fluorescence spectroscopy technique as a standard optical method for discrimination of normal and malignant tissue samples and, (ii) Optimization of the technique by the method of matching of a sample spectrum with calibration sets of spectra of pathologically certified samples. Laserinduced fluorescence spectra were measured using samples from 62 subjects at different excitation wavelengths. Principal component analysis (PCA) of spectra and intensity ratios of curveresolved fluorescence peaks were tested for discrimination. It was found that PCA of total fluorescence at 325 nm excitation gives specificity and sensitivity over 95%. Use of calibration sets of spectra of histo-pathologically certified samples combined with PCA for matching and pass/fail classification of test samples is shown to have high sensitivity/specificity for routine diagnostic purposes as well as for possible staging of the disease. Further, the multi-component origin of the fluorescence spectra is illustrated by curve resolution and fluorescence spectra of separated proteins of tissue homogenates.
In-vivo fluorescence and reflectance imaging of human cervical tissue
Medical Imaging 2003: Physiology and Function: Methods, Systems, and Applications, 2003
A hyperspectral imaging spectrograph has been used to measure the fluorescence and reflectance of cervical tissue in vivo. The instrument was employed in a clinical trial in Vilnius, Lithuania, where 111 patients were examined. The patients were initially screened by Pap smear, examined by colposcopy and a tissue sampling procedure was performed. Detailed histopathological assessments were performed on the biopsies, and these assessments were correlated with spectra and images. The results of the spectroscopic investigations show that different tissue types within one biopsy region exhibit different spectral signatures. A spectral analysis of the entire image localizes dysplastic regions in both fluorescence and reflectance, suggesting that the hyperspectral imaging technique is useful in the management of cervical malignancies.
Journal of Biomedical Optics, 2013
We have assessed the value of single fiber reflectance (SFR) spectroscopy in prediction of cervical squamous intraepithelial lesions (SIL). SFR was used to measure reflected light from 32 patients undergoing standard colposcopy. Seven parameters extracted from the spectra in addition to two biographic parameters were compared in biopsy-confirmed SIL versus nonSIL. The significant parameters in the model were determined using stepwise logistic regression. The classification performance was evaluated by a leave-one-out cross-validation method and reported by receiver operating characteristic (ROC) curves. Light absorption properties and biographic characteristics of the patient contributed significantly to the accuracy of the model. Combining important parameters, the best retrospective sensitivity, specificity and area under the ROC curve for SIL sites versus nonSIL were 89%, 80% and 0.89%, respectively. SFR spectroscopy shows promise as a noninvasive, real-time method to guide the clinician in reducing the number of unnecessary biopsies. Discrimination of SIL from other abnormalities compares favorably with that obtained by fluorescence alone and by fluorescence combined with reflectance spectroscopy while the simplicity and low cost of the presented system are dominant.
2012
Optical spectroscopy has been proposed as an accurate and low-cost alternative for detection of cervical intraepithelial neoplasia. We previously published an algorithm using optical spectroscopy as an adjunct to colposcopy and found good accuracy (sensitivity ¼ 1.00 [95% confidence interval ðCIÞ ¼ 0.92 to 1.00], specificity ¼ 0.71 [95% CI ¼ 0.62 to 0.79]). Those results used measurements taken by expert colposcopists as well as the colposcopy diagnosis. In this study, we trained and tested an algorithm for the detection of cervical intraepithelial neoplasia (i.e., identifying those patients who had histology reading CIN 2 or worse) that did not include the colposcopic diagnosis. Furthermore, we explored the interaction between spectroscopy and colposcopy, examining the importance of probe placement expertise. The colposcopic diagnosis-independent spectroscopy algorithm had a sensitivity of 0.98 (95% CI ¼ 0.89 to 1.00) and a specificity of 0.62 (95% CI ¼ 0.52 to 0.71). The difference in the partial area under the ROC curves between spectroscopy with and without the colposcopic diagnosis was statistically significant at the patient level (p ¼ 0.05) but not the site level (p ¼ 0.13). The results suggest that the device has high accuracy over a wide range of provider accuracy and hence could plausibly be implemented by providers with limited training.
2019
Cancer diagnosis by non-invasive techniques is subject of cutting-edge research in biomedical field. This paper presents experimental results of absorption and reflectance spectroscopy for ex-vivo assessment of cancerous and pre-cancerous cervical tissue. Results show promising and provide a methodology to be integrated with the standard papsmear or screening and tests aimed at preventing deaths due to cervical cancer