American Fisheries Society Special Publication 18:304-310, 1988 © Copyright by Ihe American Fisheries Sociely 1988 Monoclonal Antibodies: A Tool for Molluscan Pathology (original) (raw)
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Monoclonal Antibodies: A Tool for Molluscan Pathology
1988
Hybridoma technology is reviewed, and the characteristics of monoclonal antibodies are compared with those of polyclonal antibodies. The contribution of monoclonal antibodies to molluscan pathology is developed with special emphasis on their use as diagnostic tools. The results of studies with monoclonal antibodies prepared against the protozoan oyster pathogen Bonamia ostreae are briefly described.
A Novel Monoclonal Antibody That Binds to Hemocytes from Shrimps and Oysters
Hybridoma, 2010
The monoclonal antibody (MAb) LITO-1 was produced from a stable hybridoma cell line generated by the fusion of NS1 myeloma cells with spleen cells isolated from Balb=c mice immunized with a paraformaldehydefixed hemocyte suspension of Litopenaeus vannamei. This MAb reacted with all three hemocyte subtypes, but no reaction was observed with components of plasma. Immunohistochemistry assays demonstrated that LITO-1 was very effective in specifically distinguishing hemocytes infiltrated in several tissues such as striated muscle, brain, and hepatopancreas. Moreover, this antibody was able to recognize hemocytes from two shrimp species, Litopenaeus schmitti and Farfantepenaeus paulensis, as well as hemocytes of the oyster Crassostrea gigas. No reaction was observed against hemocytes from the terrestrial insect Triatoma klugi or with mammalian RAW cells. This novel MAb can be useful in revealing the presence and function of a conservative epitope in hemocytes of marine crustaceans and mollusks.
Diseases of Aquatic Organisms, 1991
A4ytdus edulis develop progressive and fatal hemocyhc neoplasia. We have generated monoclonal antibodies using neoplastic hernocytes from highly infected individuals collected from Puget Sound in Washington State (USA). Twenty-seven monoclonal antibodies against neoplashc cell antigens were obtaned. Specificity of 2 purified antibodies, 14F1 and 16G10, was demonstrated by immunofluorescence when incubated with neoplastic hemocytes, whereas these antibodies did not react with normal cells. This specificity was confirmed by immunogold labelling and electron nucroscopic visualiza~on. The technique permitted localization of the epitopes recognized by the monoclonal antibodies on the plasma membrane. These monoclonal antibodies are suitable reagents for establishment of immunoassays for diagnosis and quantification of hemic neoplasia in the mussel.
Aquaculture, 2005
Monoclonal antibodies (mAbs) were generated against 2-day-old mussel larvae in an attempt to develop a rapid and rigorous method for the identification of mussel larvae in field plankton samples. Previously, we have shown that two of these mAbs recognised Galician Mytilus galloprovincialis obtained from monospecific cultures, but did not recognise the larvae of other bivalve species present in that area. To assess the possibility of using these mAbs in routine assays for measuring the abundance of mussel larvae in plankton, studies on cultured mussel larvae, at different stages of development, and tests on bivalve larvae from plankton samples were carried out. Initially, to see whether the two mAbs also recognise other mussel larval stages, they were tested against mussel larvae of different ages obtained from monospecific cultures. The results indicate that both antibodies stain all the stages tested, even 1-month-old postlarvae. In addition, we also demonstrate that these mAbs also recognise other forms of Mytilus. Both antibodies bind to M. galloprovincialis larvae from the Mediterranean Sea and M. edulis larvae. Finally, and more significantly, studies on field plankton samples were performed to confirm if both mAbs are really mussel-specific, and do not cross-react with larvae of any other bivalve species existing in the plankton. The results presented here clearly indicate that our two monoclonal antibodies specifically recognise the mussel larvae in field plankton samples from different geographical regions, but not the larvae of any other bivalve species. Thus, these monoclonal antibodies could be used for routine monitoring of mussel larvae in plankton samples from different sources. D
Diseases of Aquatic Organisms, 1991
Monoclonal antibodies against Bonamia ostreae (Protozoa: Ascetospora), an intrahaemocytic parasite of flat oyster Ostrea edulis (Mollusca: Bivalvia) Herve Rogierl, Dominique Hervio2, Viviane ~o u l o~, Christine claviesl, Eliane Hervaudl, Evelyne ache re^, Eric ~i a l h e~, Henri ~r i z e l~, Bernard pau3, Francis ~a o l u c c i l ' Sanofi Recherche, Rue du Professeur,