Prediction of novel drug targets in Ergosterol biosynthesis pathway: A proposed mechanism of anticandidal activity of gr een tea phytocompounds (original) (raw)

The emergence of antibiotic resistance in pathogeni c Candida species has led to explore new alternativ e therapies to combat its associated life threatening infection s. Synergistic anticandidal potentials of green tea (derived from Camellia sinensis) with antifungal agents is well k nown. Our study aims at predicting the novel inhibi tory targets of green tea (GT) phytocompounds and pretending the in ibitory mechanism involved in synergistic inhibiti on of GT with antifungals. The interaction of GT phytocompou nds and ergosterol synthesising proteins (ERG) of t he Candida species has been assessed by in silico study using iGEMDOCK software which revealed ERG 26, ERG 6, ERG 25, and ERG 8 proteins as novel drug targets of kaempfe ritrin, EGCG, ECG, chlorogenic acid respectively pr esent in GT.Supporting our investigation, in vitro studies h ave been done with GT leaves from different geograp hic l locations. Catechins were purified and identified b y HPLC and synergistic effect of s...