Meningitis Associated with Brucella abortus in an Aborted Bovine Foetus (original) (raw)
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Abortion is the most obvious manifestation of Brucella infection. In this study, 59 aborted buffalos, 91 aborted cows and 150 aborted women were studied. Diagnosis of Brucella infection in these abortions was based on clinical, serological and bacteriological studies, then determination of Brucella isolates with PCR assay. Serological studies included the use of RB and ELISA tests as screening tests for infection. Results showed that in bovine the RB test shows significant difference between the positive cases in cows and buffalos (P<0.05) while in ELISA test there is no significant difference between these two groups. In aborted women there was significant difference in the RB and ELISA tests between the negative and positive women (P<0.05).From all positive cases by ELISA test, Brucella was isolated from 7 aborted cows, 3 buffalos, and 2 women. Brucella isolates were revealed amplification of a 223-bp fragment with B4 and B5 primers except one strain that isolated from blood culture of women .
Abortion is the most obvious manifestation of Brucella infection. In this study, 59 aborted buffalos and 91 aborted cows were included. Diagnosis of Brucella infection in these abortions was based on clinical, serological, bacteriological, hormonal, and molecular assays. Serological studies included the use of RB and ELISA tests as screening tests for infection. Argumentative differences between RB and ELISA results have been shown. Brucella isolated and identified from aborted fetuses, vaginal discharge and milk samples were 7 isolates from aborted cows and 3 from aborted buffalos. Brucella isolates revealed amplification of a 223-bp fragment with B4 and B5 primers. Hormonal assessment in both, brucella infected cows and buffalos, registered significant decrease in progesterone and P/E ratio in comparison with that aborted due to other causes. Immunohistochemical study revealed down expression level of 3BHSD enzyme in placentas of Brucella positive animals. ELISA technique was the valuable serological test to confirm the diagnosis of brucellosis. In conclusion, both RB and ELISA are necessary to be performed together as screening tests in diagnosis of brucellosis, whereas serum hormonal, placental immunohistochemical, and molecular (PCR) assessments have an efficient diagnostic values which can be included for confirmation of brucellosis.
Immunohistochemical and Microbiological Detection of Brucella abortus in Aborted Bovine Fetuses
Acta Veterinaria Brno, 2004
Twenty-five bovine fetuses naturally infected with Brucella abortus were morphologically and immunohistochemically evaluated in association with bacteriologic culture. Histopathological changes were mainly bronchopneumonia in the lungs, lymphoid hyperplasia and lymphoreticular hyperplasia in the liver and spleen. Histopathologic changes in other organs and tissues revealed hematogenous spread of the infection. Immunoreactivity to Brucella abortus was detected in all the lungs (25 fetuses) examined. However, the antigen was not detected in any of the thymus examined. Intracellular antigenic localization was identified mainly in macrophages, neutrophils and hepatocytes. In addition, B. abortus strains were isolated from abomasal contents and lungs of 22 fetuses. Eighteen of the strains were biotype 1 and the remaining four were biotype 3. These findings indicate the usefulness of immunohistochemistry in suspected cases where bacteriologic culture is negative and in cases where serology is not possible or material fixed in formalin.
Revista Mexicana de Ciencias Pecuarias, 2003
The specificity and sensitivity of different serological brucellosis diagnostic tests was studied in vaccinated Holstein cattle living within an infected herd. All seronegative animals were revaccinated with a reduced dosage (3 x 109 cfu) Brucella abortus strain 19 vaccine. A total of 73 adult female cows were studied. Six were found to be brucellosis-positive and were not revaccinated, the remaining 67 were revaccinated with a reduced dose of strain 19. Serum samples were taken on days 0, 30, 60, 90, 180 and 270 after revaccination. Samples were tested with card test, Rivanol, complement-fixation (CF), radial immunodiffusion (RID), indirect ELISA (ELISA-I) and competitive ELISA (ELISA-C). A bacteriological study was done of milk samples collected only on day 0. Brucella abortus biotype 1 was isolated from the six cows found positive with ELISA-C and RID. The highest numbers of seropositive reactions were recorded between days 30 to 270, using the card, Rivanol, CF and ELISA-I tests...
Pathogenesis of abortion of bovine brucellosis
Comparative Immunology, Microbiology and Infectious Diseases, 1993
Bovine brucellosis is a major disease of cattle characterized by abortion during the last trimester of gestation. During many years important pieces of research have been done looking for a better understanding of this particular phenomenon. Yet, the fact that the abortion takes place in the last period of gestation result in a fascinating interrogant for such a unique event. The present review includes most of the information available regarding to this matter. Emphasis is done in the interaction of Brucella abortus with the trophoblastic cells of the bovine placenta.
IJVST-2019; Vol.11, No.2, 2019
to investigate risk factors of bru-cellosis in aborted cattle in Jimma zone. During the study period, 141 cases and 282 controls were selected to assess and compare the presence of anti-Brucella antibodies between cases and controls. Cattle that had experienced abortion were defined as cases, whereas controls were cattle that had no record of abortion. Sera samples were collected from both cases and control cattle groups for laboratory tests (serological test). The existence of the anti-Brucella antibodes in serum samples was first tested by the Rose Bengal Plate test, and the all positive samples were confirmed using the complement fixation test. An overall of 4.02% seropreva-lence of brucellosis was recorded in the study areas. Antibody against Brucella organism was higher among cases (6.38%) than controls (2.84%). Multivariable logistic regression analysis identified age (OR 14.16, CI= 2.91-28.84), breed (OR 5.36, CI= 1.76-11.33), herd size (OR 11.82, CI= 1.31-16.17) and species composition (OR 5.10, CI=1.49-13.43) as risk factors (p < 0.05) for Brucella seropositivity. This study documented the occurrence of cattle brucellosis in study areas. Thus, applicable control methods and creating public awareness on the zoonotic transmission of brucellosis should be conducted. Moreover, further study considering more causes should be carried out to identify the specific causes of abortion in cattle for the preparation of the appropriate vaccine. a b b
Abortion and shedding of Brucella abortus RB51 in vaginal exudates of vaccinated cows
2005
In this study, Brucella abortus RB51 could be detected in the vaginal exudates of 5 out of 26 aborted cows following vaccination of three dairy cattle herds of 3810 animals including heifers and pregnant and non-pregnant cows by RB51 vaccine. Abortion associated with isolation of RB51 vaccinal strain from recently vaccinated cows in this study served as evidence that the vaccinal RB51 strain was the cause of abortion due to extensive bacterial multiplication and colonization in the uterus of vaccinated pregnant cows.
An overview of brucellosis in cattle and human
2021
Bacteria of the genus Brucella cause brucellosis, one of the world’s neglected zoonotic diseases. The infection of the disease cause lifetime sterility in human beings. In recent time, there is an in-infection rates between and within the species. Lacking in the resources, poor management and lack of awareness are major factors in the spread of the disease. Abortion in the last trimester is a predominant sign, of the disease in cattle. Besides this, reduced milk yield and high temperature is also observed in the affected animals, while in humans it is characterized by undulant fever, general malaise, and arthritis. While the clinical picture of brucellosis in humans and cattle is not clear and often misleading. In this review, several aspects of management, diagnosis, control, treatment, and preventive aspects are reviewed.
Journal of Veterinary Diagnostic Investigation, 1998
A commercially available polyclonal antibody and an avidin-biotin-peroxidase immunohisto-chemical technique were used to detect Brucella abortus antigens in formalin-fixed, paraffin-embedded tissues of lung and liver from 20 aborted bovine fetuses. Thirteen fetuses were obtained from farms with a previous history of brucellosis, and 7 were collected from farms without a history of brucellosis. Among the 13 aborted bovine fetuses obtained from farms with a history of brucellosis, immunoreactivity to B. abortus was detected in lung (9 fetuses) and in liver (1 fetus), whereas Brucella was cultured from abomasal contents in 9 fetuses (8 were immunohistochemically positive). In addition, 11 dams of these 13 aborted bovine fetuses had antibodies to Brucella. Brucella abortus was not detected by immunohistochemistry in the 7 aborted bovine fetuses collected from farms without a history of brucellosis. Bacteriologic culture and serologic tests were also negative for Brucella. The results of...