Characterizing sources of variability in zebrafish embryo screening protocols_suppl (original) (raw)

cial use. The need for toxicity information on these chemicals is driving interest in adopting test methods with higher throughput (Tice et al., 2013; Bugel et al., 2014). In response to a number of factors, including the National Research Council report Toxicity Testing in the 21 st Century (NRC, 2007), testing initiatives such as the U.S. government's interagency Tox21 project (Attene-Ramos et al., 2013; Tice et al., 1 Introduction Traditional mammalian toxicity tests are time-intensive, expensive, and require both large amounts of test chemical and large numbers of animals (NRC, 2007; Rovida and Hartung, 2009). The cost and time needed to conduct these tests may limit the toxicity data available for the thousands of chemicals in commer