A fast and reliable method for detecting SNP rs67384697 (Hsa‐miR‐148a binding site) by a single run of allele‐specific real‐time PCR (original) (raw)

HLA, 2020

Abstract

Surface expression of HLA-class I molecules is critical for modulating T/NK lymphocytes' effector functions. Amongst HLA molecules, HLA-C, the most recently evolved form of class I antigens, is subjected to both transcriptional and multiple post transcriptional regulation mechanisms affecting its cell surface expression. Amongst the latter a region placed in the 3' untranslated region (UTR) of HLA-C transcript contains the single nucleotide polymorphism (SNP) rs67384697 "G-ins/del" that has been found to be strictly associated with surface levels of HLA-C allomorphs due to the effect on the binding site of a microRNA (Hsa-miR-148a). Higher expression of HLA-C has proved to influence HIV-1 infection via a better control of viremia and a slower disease progression. More importantly, the analysis of SNP rs67384697 "G-ins/del" combined with the evaluation of the HLA-Bw4/-Bw6 C1/C2 supratype, as well as the KIR genetic asset, has proved to be pivotal in defining the status of Elite Controllers in the caucasian population. Here we describe a new reliable and fast method of allele-specific real-time PCR to monitor the integrity/disruption of the binding site of the microRNA Hsa-miR-148a in a high-throughput format that can be easily applied to studies involving large cohorts of individuals. This article is protected by copyright. All rights reserved.

Donato Zipeto hasn't uploaded this paper.

Let Donato know you want this paper to be uploaded.

Ask for this paper to be uploaded.