Triperpenes in the callus culture of Vitex negundo L (original) (raw)

2005

Abstract

Vitex negundo L. is a pantropic herb which can be found in South and Southeast Asia. Known locally in the Philippines as “lagundi”, V. negundo is a popular medicinal plant that is used as an antitussive, antiasthma and antipyretic (Quisumbing 1978, Pardo de Tavera 2000). A number of compounds have been isolated and identified from the leaves, including sesquiterpenes (β-eudesmol) (Dayrit & Trono 1995), flavonoids (casticin, chrysoplenol D, luteolin and isoorientin) (Dayrit et al.1987), iridoids (2’-para-hydroxybenzoyl mussaenosidic acid, 6’-parahydroxybenzoyl mussaenosidic acid, agnuside and lagundinin) (Dayrit & Lagurin 1994), and minor amounts of the plant steroid β-sitosterol (Joshi et al. 1974). Many other monoterpenes and sesquiterpenes had also been detected in smaller amounts by GC-MS (Mallavarapu et al. 1994). A callus line of Vitex negundo was established using explants from the leaves. The calli showed stable morphology and thin layer chromatographic (TLC) profiles over se...

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