Effect of Extraction Methods on Polyphenols, Flavonoids, Mineral Elements, and Biological Activities of Essential Oil and Extracts of Mentha pulegium L (original) (raw)
Related papers
2015
The present study describes the total phenolic and flavonoids content, and in vitro antibacterial activity of petroleum ether, ethyl acetate and n-butanolic extracts from Mentha pulegium L. growing in Tarik Ibn Ziad within the region of Ain-Defla located in northern Algeria. The harvesting of the plant is made for three months: February, March and April 2013. We chose four bacterial strains common in human pathology, belonging to Gram positive and Gram negative classes. Bacterial strains used were: Pseudomonas aeruginosa (Gram negative), Escherichia coli (Gram negative), Klebsiella pneumoniae (Gram negative) and Staphylococcus aureus (Gram positive). These bacterial species are responsible for skin infections (Staphylococcus aureus), urinary and digestive tract infections (Escherichia coli), and nosocomial infections (Klebsiella pneumoniae and Pseudomonas aeruginosa). Antimicrobial was performed with the Bauer-Kirby method by the well diffusion technique using Petri plates made by M...
The analysis and identification of essential oils hydrodistilated from two mint species (Mentha spicata and Mentha pulagium) by means of gaz chromatography and mass spectroscopy was realized in this investigation. In addition, their antagonistic activity against some pathogenic bacteria was screened. Acceptable yield (1.0 and 0.87 % for M. Pulagium and M. Spicata respectively) was obtained. From the leaf essential oil Mentha spicata 57 compounds were separated, representing 97,022% of the total essential oil mass from which 44 compounds were elucidated. The major compound was carvone (59.40 %), other components present in appreciable contents were: limonène (6.12%), 1,8-cinéol, germacrène-D (04.66%), β-caryophyllène (2.969 %), β-bourbonène (2.796 %), α-terpinéol (1.986 %), Terpinéne-4-ol (1.120 %). The essential oil of Mentha pulegium, 43 compounds were separated, representing 99.52 % of the total essential oil mass from which 29 compounds were elucidated. The major component was pu...
American Journal of Plant Sciences, 2015
The essential oils from Mentha viridis L. and Mentha pulegium L. were characterized by gas chromatography coupled to mass spectrometry (GC-MS). These oils were obtained by hydrodistillation and presented linalool (40.70%), carvone (13.52%) and α-terpinene (8.56%) as the principal constituents in the essential oil from Mentha viridis L. Pulegone (50.01%), menthol (31.90%) and menthone (16.56%) were the principal constituents in the essential oil from Mentha pulegium L. These essential oils (in concentrations ranging from 3.91 to 500 μL·mL −1 ) showed satisfactory activities against Escherichia coli, Listeria monocytogenes, Salmonella choleraesuis and Staphylococcus aureus. The antioxidant activities with 2-deoxyribose and phosphomolybdenum and the reducing power (in concentrations ranging from 0.78 to 1000 μL·mL −1 ) were determined. The antioxidant activity was observed for the two oils evaluated by the phosphomolybdenum and 2-deoxyribose methods, whereas the essential oil from M. viridis presented low antioxidant activity in the reducing power assay.
Evidence-based Complementary and Alternative Medicine : eCAM, 2021
In the present study, the relationship between the phenolic counts, chemical composition, and biological activities of two Mentha species (Mentha rotundifolia (MR) and Mentha pulegium (MP)) was analyzed. The characterization of the action mode against pathogenic bacteria and the inhibition of spore germination of two fungal species using prepared methanolic extracts were studied here for the first time. The obtained data highlighted the presence of positive correlation between the secondary metabolites contents and the biological activities of the investigated extracts. In fact, HPLC analysis showed that the major components in both the extracts were eriocitrin and rosmarinic acid (25 and 20 mg/ml and 12 and 8 mg/ml in methanolic extracts of MR and MP, respectively). Moreover, the MR extract was rich in polyphenols and presents the highest antioxidant activity than MP ones. In addition, both extracts possess an antimicrobial activity against four Gram-positive and five Gram-negative...
In vitro antioxidant activity of essential oil of aerial parts of Mentha pulegium L
Acta Agriculturae Serbica, 2020
This work was conducted to evaluate the antioxidant activity of the essential oil obtained from the aerial part of Mentha pulegium. The antioxidant power of the essential oil obtained by hydrodistillation using a Clevenger-type apparatus was evaluated by using three methods: free radical-scavenging activity, reducing power and liver lipid peroxidation assay. Results showed that Mentha pulegium oil displayed good quality according to its physicochemical characteristics, and a higher yield 5.1 ± 0.2%. The essential oil of Mentha pulegium showed a higher DPPH radical scavenging activity 90.54 ± 1.5 % at a concentration of 1000 µg/mL. This value was close to the results obtained with ascorbic acid 96.23 ± 1.2%, and catechin 94.50 ± 1.4%. This oil exhibited significant potential for reducing iron (the value observed by optical density was 1.8 ± 0.3), while ascorbic acid and catechin provided an OD of 2.069 ± 0.03 and 2.66 ± 0.016 at the same concentration of 1000 µg/mL. The tested oil pr...
RADICAL SCAVENGING AND DISINFECTANT EFFECT OF ESSENTIAL OIL FROM MOROCCAN MENTHA PULEGIUM
International Journal of Pharmacy and Pharmaceutical Sciences, 2016
Objective: As part of the valorization of medicinal and aromatic plants in Morocco, this study aims to investigate the chemical composition, the antimicrobial and the antioxidant activities of Mentha pulegium essential oil. Methods: Essential oil of M. pulegium from Oued Laou region (Morocco) was screened for its antimicrobial activity against seven bacteria and two fungi using broth microdilution method. The radical scavenging effect was used to assess in vitro antioxidant capacity of this oil. Results: The tested essential oil exhibited strong inhibitory effect against all tested microorganisms with minimum inhibitory concentrations ranging from 0.003% to 1% (v/v) except for Pseudomonas aeruginosa. Moreover, the studied essential oil exhibited radical scavenging power with the half maximal inhibitory concentration of 2 mg/ml. Conclusion: These findings showed that the studied essential oil has both an antimicrobial and antioxidant potential, which is very rare. Thus, it reinforces its use as an effective, eco-friendly preservative agent in food and cosmetic field.
Industrial Crops and Products, 2016
The essential oil obtained by hydrodistillation from the aerial parts of Origanum minutiflorum O Schwarz & PH Davis, an endemic species in Turkey, was analysed for its antimicrobial activity in vitro. Gas chromatography/mass spectrometry analysis of the essential oil resulted in the identification of 34 constituents accounting for 961.5 mL L −1 of the oil, the major compounds present being carvacrol (793.4 mL L −1), p-cymene (32.6 mL L −1) and γ-terpinene (21.4 mL L −1). The in vitro antimicrobial activity of the essential oil was investigated in order to evaluate its efficacy against 16 bacteria and two yeasts, using disc diffusion and minimum inhibitory concentration methods. The essential oil showed strong antimicrobial activity against all test microorganisms except Pseudomonas aeruginosa. Its main components carvacrol and p-cymene were also assayed for their antimicrobial activities. Carvacrol exhibited comparable activity to the crude oil, proving it to be the main component responsible for the biological activity observed. This study demonstrates the in vitro antimicrobial activity of the essential oil of this endemic remedy against a wide spectrum of clinically important microorganisms, including pathogenic yeasts, being the first report on the anticandidal properties of the essential oil of O. minutiflorum.
The current work attempts to explore the influence of three extraction solvents on phytochemical composition, content of polyphenols, antioxidant potential, and antibacterial capacity of Moroccan Mentha longifolia L. leaves hydroethanolic, acetonic, and water extracts. To achieve this goal, the chemical composition was identified using an HPLC-DAD examination. The contents of polyphenolswere assessed, while the total antioxidant capacity (TAC), the DPPH test, and the reducing power test (RP) were utilized to determine antioxidant capacity. To assess the antibacterial activity, the microdilution technique was carried out to calculate the minimum inhibitory (MIC) and minimum bactericidal concentrations (MBC) ofextracts against four nosocomial bacteria (Bacillus cereus, Pseudomonas aeruginosa, Escherichia coli, andStaphylococcus aureus).Additionally, the antibacterial and antioxidant activities of all tested extracts were examined in silico against the proteins NADPH oxidaseand Bacillu...
Records of Natural Products
The way of drying the fresh herbal material influences the chemical content and the biological activities of their essential oils. The influence of the different drying methods of the herb Mentha longifolia (L.) Hudson on the antioxidant and antimicrobial activity of the extracted essential oils has been analyzed in this study. Drying has been carried out in three ways: in the natural way, in the laboratory oven (45°C) and in the absorptional low-temperature condensational drier (35°C). The antioxidant activity of the essential oil has been estimated by FRAP and DPPH assays, while the antimicrobial activity has been estimated by the diffusible and micro-delusional method, testing on the nine types of bacteria and two types of fungi. The essential oil obtained from the herb dried in the natural way has shown the highest antioxidant activity and the lowest from the herb dried in the laboratory oven. Bacillus subtilis, Micrococcus luteus and Enterococcus faecalis have shown the highest...