Short Communication DETERMINATION OF TANNINS CONTENT BY TITRIMETRIC METHOD FOR COMPARISON OF DIFFERENT PLANT SPECIES (original) (raw)
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Determination of tannins content by titrimetric method for comparison of different plant species
Tannins represent a wide variety of compounds that can be found in fruits, vegetables, dry extract of red wine, dry extract of grape seeds, tea and dry not edible plants. Tannins are also known as proanthocyanidins possessing useful properties such as antioxidant, anti-apoptosis, anti-aging, anti-carcinogenic, anti-inflammatory as well as anti-atherosclerosis and cardiovascular protection. In the study 16 food products – 6 fruit and 2 vegetable dry species, 4 dry extracts of not edible and 4 dry not edible plants were analyzed for their tannins content by titrimetric method.
Journal of Applied Botany and Food Quality
Tannins occur in many field herbs and legumes, providing an immense variability in structure and molecular weight. This leads to complications when measuring tannin content; comparability of different methods is problematic. The present investigations aimed at characterizing four different tannin extracts: quebracho (Schinopsis lorentzii), mimosa (Acacia mearnsii), tara (Caesalpinia spinosa), and gambier (Uncaria gambir) and impact of storage conditions. Using photometrical methods as well as HPLC-ESI-MS, fundamental differences could be determined. Quebracho, mimosa, and gambier contained 164.3, 108.2, and 169.3 g kg−1 of tannin (calculated as procyanidin C1); tara reached 647.5 g kg−1 (calculated as epigallocatechin gallate). Alongside with compounds already described in the literature, several tannin molecules were found that have not been observed before in the analyzed sources. Extraction with hot water provided clear advantage over treatment with acetone or methanol; the organ...
Tannins: Current knowledge of food sources, intake, bioavailability and biological effects
Molecular Nutrition & Food Research, 2009
Tannins are a unique group of phenolic metabolites with molecular weights between 500 and 30 000 Da, which are widely distributed in almost all plant foods and beverages. Proanthocyanidins and hydrolysable tannins are the two major groups of these bioactive compounds, but complex tannins containing structural elements of both groups and specific tannins in marine brown algae have also been described. Most literature data on food tannins refer only to oligomeric compounds that are extracted with aqueous-organic solvents, but a significant number of non-extractable tannins are usually not mentioned in the literature. The biological effects of tannins usually depend on their grade of polymerisation and solubility. Highly polymerised tannins exhibit low bioaccessibility in the small intestine and low fermentability by colonic microflora. This review summarises a new approach to analysis of extractable and non-extractable tannins, major food sources, and effects of storage and processing on tannin content and bioavailability. Biological properties such as antioxidant, antimicrobial and antiviral effects are also described. In addition, the role of tannins in diabetes mellitus has been discussed.
Tannins Containing Medicinal Plants and It's Significance: An Overview
Journal of Emerging Technologies and Innovative Research, 2022
Tannin is very important chemical constituent present in various types of medicinal plants in different parts of world. Medicinal plants like Myrobalan, Ashoka, Arjuna, Pale catechu, Black catechu, Bahera, Amla, Pterocarpus, Amra, etc are showing different tannins types of chemical compositions. These medicinal plants are having those rich sources of tannins in their different parts such as bark, leaves, fruits, etc. Tannins containing medicinal plants exhibit various pharmacological activities like laxatives, purgatives, diuretics, cardiotonics, antioxidant, anti-diarrhoeal, sedatives, anti-diabetic, anti-rheumatism, astringent, anti-dysentery, anti-microbial, analgesic, anti-dyspepsia, etc. Some plants shows different properties to help the digestive disorders such as black catechu. Other kinds of tannin containing plants help to treat different skin disorders. Basically, three types of tannins such as hydrolysable condensed and pseudotannins are present in tannin containing medicinal plants. The current study correlates different medicinal plants containing tannin, tannin types, identification of tannins, chemical properties of tannin, tannin containing medicinal plants & its pharmacological actions.
Screening of Potential Sources of Tannin and Its Therapeutic Application
2015
Tannins are a unique category of plant phytochemicals especially in terms of their vast potential health-benefiting properties. Researchers have described the capacity of tannins to enhance glucose uptake and inhibit adipogenesis, thus being potential drugs for the treatment of non-insulin dependent diabetes mellitus. Thus, the present research was conducted to find out tannin content of food products. The percentage of tannin in various analyzed sources ranged from 0.0 to 108.53%; highest in kathaa and lowest in ker and mango bark. The percentage of tannins present in the plants, however, varies. Numerous studies have confirmed that the naturally occurring polyphenols are key factor for the beneficial effects of the herbal medicines. Isolation and identification of active constituents from plants, preparation of standardized dose & dosage regimen can play a significant role in improving the hypoglycaemic action.
Rapid Screening Method to Assess Tannin Antioxidant Activity in Food-Grade Botanical Extract
Italian Journal of Food Science, 2017
Fourier transform infrared (FTIR) spectroscopy measurements were used for the prediction of commercial tannins antioxidant capacity, i.e. DPPH, through Partial-least squares (PLS) regression. Plot of the leave-one-out full cross-validated PLS predicted scavenging activity values (DPPH %) with a good correlation (r= 0.82), proving FTIR succeeded to rapidly provide information on commercial tannins antioxidant capacity.
Extraction of Condensed Tannins from Mexican Plant Sources
Zeitschrift für Naturforschung C, 2008
Contents of total polyphenols, condensed tannins and proanthocyanidins, and their stability to various pH values and temperatures were studied in Mexican blueberry, cuautecomate fruit, garambullo fruit, aubergine, coffee pulp and residues of black grapes. Several aqueous extracts, obtained through a one-pass-extraction process, were analyzed using liquid chromatography in order to quantify the condensed tannin (proanthocyanidin) content responsible for their antioxidant activity and colour. All tested samples included high proanthocyanidin contents demonstrating that these Mexican fruits and vegetables are good sources of natural antioxidants, and they all could be considered as excellent functional foods due to their bioactivity measured as the condensed tannin level.
SENSITIVITY OF AOAC AND FOLIN'S METHOD IN ESTIMATION OF TANNINS FROM DIFFERENT PLANTS
Reseach Article, 2019
Tannins are a collective term for a variety of plant polyphenols used in the tanning of rawhides to produce leather. They are widely distributed in plants and occur in especially high amounts in the bark of certain trees and galls. Detection and estimation of tannins present in any plant product to be used as medicine becomes one of the preliminary tests for deciding the medicinal value of the plant part. For this study, the total tannin content was estimated in terms of standard tannic acid since it is one of the most common forms of tannins. The selected samples studied were fresh and dried Vitis vinifera, fresh and dried Phyllanthus emblica powder, fresh and dried Piper nigrum fruits, dried Cinnamomum zeylanicum, dried Elattaria cardamomum, dried Aloe barbadensis powder and Mangifera indica bark. Two different estimation methods were carried out for each of the aqueous and alcoholic extracts of the samples. First method is the regularly and extensively used Folin's method for test of polyphenols. Second method is the lesser known AOAC (KMnO 4) method.