Expression and Localisation of Adiponectin and Its Receptors in Human Ocular Tissues (original) (raw)

International journal of pharma and bio sciences, 2014

Abstract

Recently we have reported the elevated levels of Adiponectin (APN) in proliferative diabetic retinopathy (PDR) vitreous in comparison to macular hole. It has been assumed that APN may get exudated into vitreous from circulation but no experimental proof has been shown so far. Therefore we set out to determine the origin of APN in the vitreous.Donor eye balls were used to extract RNA and protein for real time PCR, western blotting. Five micron retinal sections were done for immunofluorescence and immunohistochemistry. Primary retinal epithelial and endothelial cells were isolated from donor eye balls aged below 50 years.APN exerts its function through two receptors, AdipoR1 and AdipoR2. Interestingly we identified the expression of APN, AdipoR1 and AdipoR2 mRNA transcripts in all ocular tissues by quantitative PCR. Immunofluorescence and western blot analysis demonstrated the presence of APN and its receptors AdipoR1, AdipoR2 in all ocular tissues. AdipoR1 and AdipoR2 were distribute...

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