Serum Metabolomics Investigation of Humanized Mouse Model with Dengue Infection (original) (raw)

2017, Journal of virology

Dengue is an acute febrile illness caused by dengue virus (DENV) and a major cause of morbidity and mortality in tropical and subtropical regions of the world. The lack of an appropriate small-animal model of dengue infection has greatly hindered the study of dengue pathogenesis and the development of therapeutics. In this study, we conducted a mass spectrometry-based serum metabolic profiling from a humanized mice (humice) model with DENV serotype 2 infection at 0, 3, 7, 14 and 28 days post infection (dpi). Forty-eight differential metabolites were identified, including fatty acids, purines and pyrimidines, acylcarnitines, acylglycines, phospholipids, sphingolipids, amino acid and derivatives, free fatty acids, bile acid, etc. These metabolites showed a reversible change trend - most were significantly perturbed at 3 or 7 dpi and returned to control levels at 14 or 28 dpi, indicating that these metabolites might serve as prognostic markers of the disease in humice. Major perturbed ...

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