Isolation of antibacterial compounds from dichloromethane fraction of Malaysian sponges Neopetrosiaexigua (original) (raw)
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Screening and Characterization of Antibacterial Compounds from Some Marine Sponge Species
Introduction Microorganisms are called as universal organism, found in everywhere; some are pathogenic or beneficial in nature. Due to the human activities increases the pollution in environment helps to emergence of many pathogens like bacteria, viruses and other unicellular organisms that causes serious diseases to humans. The living organisms include viruses, archaea, eubacteria, microscopic fungi, protozoa and unicellular algae. Among multimillions of living things, the eubacteria are predominantly causes several infections or diseases in higher plants and animals. Microorganisms such as bacteria are ubiquitous in everywhere and have the ability to do various beneficial and harmful functions. The beneficial functions of bacteria include Abstract: Objective: The antibacterial activities were done by using of various solvents such as ethanol, petroleum ether, methanol, chloroform, n-butanol and ethyl acetate extracts of various marine sponges like Callyspongia reticutis Dendy (C. reticutis), Thalysias vulpine Lamark (T. vulpine), Echinodictyum gorgonoides Dendy (E. gorgonoides) and Callyspongia diffusa Ridley (C. diffusa), Gelliodes cellaria Rao (G. cellaria) against gram positive and gram negative bacterial pathogens like Pseudomonas aeruginosa PA01 and Proteus mirabilis ATCC 7002 respectively. Methods: The nutrient agar well diffusion method is used to find the antibacterialbial activities against various sponge extracts. The functional groups of the sponge extracts are specifically mitigate bacterial metabolic intermediates determined by FTIR. Results: The antibacterial activities of the sponge crude extracts were increased with different concentrations in the agar well plates to indicate the presence zone of clearance. The crude extracts were prepared from different solvents such as the n-butanol and chloroform extract was the most effective extracts. At this stage the gram negative bacteria Proteus mirabilis (P. mirabilis) and the gram positive bacteria Pseudomonas aeruginosa (P. aeruginosa) appear to be most sensitive strain while and Escherichia coli, Bacillus cereus, Klebsiella pneumonia. The Staphylococcus aureus indicate resistance to the various tested concentrations and have response to no zone of inhibition was observed. The inhibition of microbial growth at concentration as low as ~50-150 mg/mL indicated the potent antibacterial activity of above mentioned porifera sponge extracts. Conclusions: These research works results were find critically industrially important compounds from selected sponge extracts and the functional groups of plant compounds is responsible for great antibacterial activity.
Screening and characterization of bioactive compound from Samalona island sponges, Indonesia
2015
Marine sponges contain unique chemical compounds that are potential to be developed as new drugs. This study aimed to screen and characterize bioactive compounds of sponges obtained from Samalona Island, South Sulawesi. The sponges were extracted with methanol and partitioned with chloroform-water mixture. The bioactivity of sponge extracts was tested against Artemia salina Leach. Extracts that showed high bioactivity/cytotoxicity were fractionated using vacuum liquid chromatography and isolated using preparative thin layer chromatography. The obtained isolates were then tested against A. Salina. The isolated compound with highest bioactivity was characterized using UV light (chemical reactions and spectrophotometer UV and IR. Three out of 15 sponges collected yielded a highly toxic extracts against A. salina and the most active chloroform extract was obtained from Penares sp. sponge. Fractionation and isolation of the Penares sp extract generated an isolate with high toxicity with ...
Biological activities of the extracts from the Andaman Sea sponges, Thailand
Eurasian Journal of Biosciences, 2010
Thirty six organic extracts were prepared from eighteen marine sponges collected from the Andaman Sea, Thailand. The extracts were examined for anti-malaria, anti-Microbacterium tuberculosis, anti-herpes simplex virus, antimicrobial, anti-acetylcholinesterase enzyme and cytotoxic activities. Four extracts showed anti-M. tuberculosis, one anti-malarial, twenty four antimicrobial and one extract exhibited cytotoxic activity. However, anti-acetylcholinesterase enzyme and anti-herpes simplex virus type 1 (HSV-1) activities were not recorded. Dichloromethane extracts prepared from Axinyssa sp., Halichondria sp. and Chondrosia reticulata exhibited potential anti-M. tuberculosis at MIC 50, 100 and 200 mg/mL, respectively. The Hexane part of Phakellia ventilabrum extract showed anti-malarial activity (MIC= 2.8 mg/mL) while the dichloromethane extract showed anti-M. tuberculosis and cytotoxic activity with MIC 200 and IC 50 7.1 mg/mL, respectively. Antimicrobial activity was found in both the hexane and dichloromethane parts of extracts.
Journal de Mycologie Medicale, 2010
Aqueous and organic extracts from four marine sponges collected from the coastline of Malaysia were screened against six microorganisms, including the bacteria Staphylococcus aureus ATCC25923, Bacillus cereus ATCC11778, Pseudomonas aeruginosa ATCC27853, Escherichia coli ATCC35218, the yeasts Candida albicans ATCC10231 and Cryptococcus neoformans ATCC90112. All the sponge species in this study showed antibacterial activity against at least one bacterial strain and only one sponge species was significantly active against C. albicans. P. aeruginosa was considered resistant to all tested samples, since no inhibition zone was observed while the Gram-positive B. cereus was shown to be the most sensitive microorganism followed by C. albicans and S. aureus. The highest activity was obtained for the aqueous extract of Neopetrosia exigua against the Gram-positive bacteria B. cereus (inhibition zone 25 mm and MIC 0.07 mg/mL) and S. aureus (17.5 mm and 0.12 mg/mL) and against C. albicans (21 mm and 0.32 mg/mL). This extract is currently undergoing further analysis to identify the active compounds. # 2010 Elsevier Masson SAS. All rights reserved.
The collected alkaloid compounds of Gelliodes carnosa sponge species from depth of 5-6 meters of Nay band Bay (Iran's coasts of Persian Gulf) were extracted by three different methods The obtained extract was tested on two gram-positive bacteria (Staphylococcus aureus, PTCC 1189, Bacillus subtilis, PTCC 1156), and five gramnegative bacteria (Escherichia coli,PTCC 1763, Pseudomonas saeroginosa, PTCC 1310, Proteus mirabilis, PTCC 1076, Serattia marcescens, Klebsiella pneumonia), and six pathogenic fungi (Candida albicans, PTCC 5027, Fusarium solani, PTCC 5248, Aspergillus niger, PTCC 5223, Saprolegnia sp., Fusarium sp.1, Fusarium sp.2). The extracted alkaloid compounds from the three methods had a significant inhibitory activity on the growth of all the bacteria and fungi. The anti-bacterial properties of these compounds were more than their anti-fungal properties.
Bioactive Compounds from Marine Sponges: Fundamentals and Applications
Marine Drugs
Marine sponges are sessile invertebrates that can be found in temperate, polar and tropical regions. They are known to be major contributors of bioactive compounds, which are discovered in and extracted from the marine environment. The compounds extracted from these sponges are known to exhibit various bioactivities, such as antimicrobial, antitumor and general cytotoxicity. For example, various compounds isolated from Theonella swinhoei have showcased various bioactivities, such as those that are antibacterial, antiviral and antifungal. In this review, we discuss bioactive compounds that have been identified from marine sponges that showcase the ability to act as antibacterial, antiviral, anti-malarial and antifungal agents against human pathogens and fish pathogens in the aquaculture industry. Moreover, the application of such compounds as antimicrobial agents in other veterinary commodities, such as poultry, cattle farming and domesticated cats, is discussed, along with a brief d...
Screening of Antimicrobial Activity of Sponges Extract from Pasir Putih, East Java (Indonesia)
Journal of Marine Science: Research & Development, 2017
The emergence of new infectious diseases, the resurgence of several infections that appeared to have been controlled, and the increase in bacterial resistance have created the necessity for studies directed towards the development of new antimicrobials; considering the failure to acquire new molecules with antimicrobial properties from marine sponges. The objective of this study was to evaluate screening of antimicrobial activity of seven sponges extract from Pasir Putih, East Java against some Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) and Gram negative (Escherichia coli and Klebsiella pneumoniae) as well as drug-resistant bacteria (S. aureus and Pseudomonas aeruginosae) using the agar diffusion method and phytochemical screening of the extract. The findings show that the extract, Xestospongia testudinaria has a stronger antibacterial activity against bacterial pathogens S. aureus, E.coli, K. pneumoniae, Salmonella typhi and bacteria resistant P. aeruginosa MDR and S. aureus MRSA compared to other sponge extract. In conclusion, the showed X. testudinaria ethanol extract was more active than other sponge extracts.
Screening of antimicrobial activity of marine sponge extracts
Journal De Mycologie Medicale, 2005
The antimicrobial activity of the chloroform extracts of ten marine sponges collected from the Atlantic coast of Morocco and from the Gulf of Thailand was tested against four bacterial species and five fungal species using the agar disk method. Extracts tested showed antimicrobial activity against at least one of the test strains, 50% of them showed antibacterial activity while only 20% exhibited antifungal activity. Among them, the extracts of Hippospongia communis and Ircinia variabilis collected from the Atlantic coast of Morocco appeared to be quite promising due to their capacity to inhibit the growth of Candida tropicalis R 2 (an amphotericin B and nystatin resistant strain) as well as their broad spectrum activity against other bacteria and fungi. The antifungal activity of the extracts was further characterized by determining the minimum inhibitory concentration (MIC) against four fungal species selected using the broth microdilution method and two test media.