Biodegradation of Phenol by Bacillus simplex: Characterization and Kinetics Study (original) (raw)

Biodegradation of phenol by bacterial strains and their catalytic ability

The capability of azo dye degrading strains, designated as S1, P-NA, P-NY, and A-NA was investigated for their ability to degrade phenol. The strains were identified as Bacillus cereus S1, Brevibacillus spp. P-NA, Bacillus sp. P-NY, and as Brevibacillus sp. A-NA based on 16S rRNA gene sequencing and phylogenetic analysis. The strains acclimatized well to increasing concentration of phenol (200-1000 mg/l) on MSM media in the presence of 1% glucose. The strains were having best phenol degrading ability at pH 7.2 and 37 0 C. The biodegradability of phenol was confirmed through HPLC and GC-MS analysis. Catechol and formation other metabolic intermediates of phenol degradation, identified by comparing their retention time (RT in NIST library (National Institute of Standard and Technology), and predominant activity catechol 2, 3-dioxygenase indicated by meta-cleavage pathway.

Biodegradation of phenol by a native mixed bacte 2017 International Biodeter

An efficient phenol degrading mixed bacterial culture was isolated from sludge sample collected from one of the refinery located in Assam, India. The mixed culture was found to consist of three bacterial strains. These were identified as Stenotrophomonas acidaminiphila, Brevibacterium sp. and Brucella sp. Batch phenol biodegradation experiments were carried out for a wide range of initial phenol concentrations after pH and temperature optimization. It was found that the mixed culture was able to degrade a maximum phenol concentration up to 1000 mg L À1 within 96 h while the maximum specific growth rate (m max ) was observed at 100 mg L À1 . The pH and temperature required for optimal phenol degradation was 6.5 and 37 C respectively. The mixed culture degrades phenol via ortho-cleavage pathway by formation of an intermediate (cis, cis-muconate) which was detected spectrophotometrically at 260 nm. The experimental data were validated by fitting the growth and substrate utilization curves with their corresponding simulated dynamic profiles obtained by solving Haldane's equation via MATLAB R2015a with m max ¼ 0.155 h À1 and K I ¼ 400 mg L À1 .

Biodegradation of phenol by alkaliphiic Bacillus badius D1

Phenols have been recognized as mutagenic and carcinogenic. Industrial effluents possessing phenols causes' environmental pollution. Present study was planned to investigate the % degradation of phenol using alkaliphilic Bacillus badius D1 with various concentration ranging from 0.42 to 1.68 g/L in alkaline broth of pH-9, in shaking incubator at110 rpm, 37 0 C. The % degradation at lowest concentration was about 98%. Even at higher concentration the rate of degradation was found higher than 70 %. The % degradation and spectroscopic profile was monitored from 0 to 48 hr. by 6 hr. interval. The metabolic pathway was constructed by isolating and characterizing the metabolites by 1 HNMR, FTIR, and GCMS. It was concluded that the Bacillus badius follows ortho and meta catechol pathway for degradation. The biodegradation study was extended for varying parameters like temperature, pH, salinity, additional carbon and nitrogen sources.

Biodegradation of Phenol: Mini Review

Journal of Basic and Applied Research in Biomedicine, 2020

This paper is a comprehensive review related to the biological degradation of phenol by microorganisms. The aromatic compound, phenol or hydroxybenzene, is produced industrially or naturally. Many microorganisms that are able to biodegrade phenol have been isolated and at the same time, the metabolic pathways responsible for these metabolic processes have been determined. A large number of bacteria were studied in detail especially, pure cultures as well as the pathways of aerobic phenol metabolism and the enzymes involved. Phenol oxygenation occurred as the initial steps through phenol hydroxylase enzymes leading to formation of catechol, pursued by the splitting of the adjacent ring or in between the two groups of catechol hydroxyls. Thus, the physical and chemical environments plus the chemical structures that affecting biodegradation processes are important determining factors for combating of pollution. This nature of chemical structure for the other aromatic compounds is also a main decisive factor of biodegradability.

Biodegradation of Phenol: A Review

Phenol is one of the organic pollutants in industrial waste water which causes significant environmental problems. Various methods such as chlorination, flocculation, adsorption etc. have been used for the degradation of phenol. But biological methods have proved to be the most effective and economical approach for the removal of phenol and phenol related compounds. Numerous studies on biodegradation of phenol using different types of microorganisms and bioreactors have been reported. Various kinetic models and the effect of various parameters such as initial phenol concentration, temperature, pH, etc. on biodegradation have been also studied.

Biodegradation of Phenol by Native Bacteria Isolated From Dioxin Contaminated Soils

Journal of Bioremediation & Biodegradation, 2012

Phenol is an environmental pollutant present in industrial wastewaters such as refineries, coal processing and petrochemicals products. In this study three phenol degrading bacteria from Arak Petrochemical Complex effluent were isolated which consume phenol. Molecular analysis was used to identify bacteria and isolated bacteria were identified as Rhodococcus pyridinivorans (NS1), Advenella faeciporci (NS2) and Pseudomonas aeroginosa (NS3). Among the isolated strains, NS1 had the highest ability to degrade phenol. In order to observe the best yield in phenol biodegradation using NS1, optimization was performed using one factor at a time of experimental design to investigate the effect of four factors, including pH, temperature, phosphate and urea concentration. The optimal biodegradation condition through or tho pathway was pH = 8, urea = 1 g/L, temperature = 30°C and K 2 HPO 4 = 0.5 g/L. Under the suggested condition, a biodegradation efficiency of 100% was achieved. Moreover, NS1 has shown growth and phenol degradation in concentrations between 250 to 2000 mg/L. In a nutshell, the results revealed that phenol efficiently consumed by NS1 as the sole carbon source. Obviously, the isolate strain may be seen as an important tool in the bioremediation of wastewater effluent, petrochemical complex.

Anaerobic biodegradation of 13C6-phenol: Analysis of bacteria population involved in two different degradation kinetics

Biodegradation of 13C6-phenol until mineralisation was observed under mesophilic and thermophilic conditions during the degradation of municipal solid waste. In Mesophilic conditions, as well as in thermophilic, two types of degradation kinetics were obtained. One follows a first order kinetic and the other follows a lag-phase model. The pyrosequencing analysis shows that in the fastest kinetic Clostridium sp. and Anaerobranca sp. were the microorganisms majorly present under mesophilic and thermophilic conditions, respectively. These microorganisms have been already referenced in the literature as microorganisms involved in phenol degradation. Our results suggest that these differences could be explained by the proportions of microorganisms present in both kinetics.