Phytochemical, Cytotoxic, and Antimicrobial Evaluation of Tribulus terrestris L., Typha domingensis Pers., and Ricinus communis L.: Scientific Evidences for Folkloric Uses (original) (raw)

Phytochemical Screening of Leaves, Stem and Fruit Parts of Tribulus Terrestris

2021

The comparative phytochemical analysis of leaves, stem and fruit powder of Tribulus terrestris was carried out by using preliminary phytochemical screening. To identify the phytochemical constituents, present in water and ethanol extracts of Tribulus terrestris leaves, stem and fruit, a preliminary analysis was done by using different testing methods of Frothing test, Mayer’s test, Hager’s test, Foam formation test, Lead acetate test, Molisch’s test and Ferric Chloride test. The phytochemical analysis study showed the presence of 3.903%, 1.725% and 2.259% terpenoids & phenolic in leaves, stem and fruit parts of Tribulus terrestris respectively. The study showed the presence of 16.574%, 15.943% and 10.168% alkaloids and 0.240%, 0.160% and 0.230% Q. alkaloid & N-oxides in leaves stem and fruit extracts respectively. 2.888%, 0.825% and 2.679% fats and waxes and 76.395%, 81.347% and 84.672% fibers were observed in leaves, stem and fruit extracts of this plant respectively

19_GC-MS Analysis and Antimicrobial Screening of Non-polar Fraction of Tribulus Terrestris.pdf

Traditionally Tribulus terrestris commonly utilized for the treatment of renal dysfunction. In present study, the phytochemical composition of non-polar fraction of ethanol extract of Tribulus terrestris twigs have been evaluated with the help of GC followed by GC-MS along with its antimicrobial activity. A total of eleven compounds were identified mainly fatty acid and their methyl esters. In order to support the identifications Kovat's Retention Indices were also calculate of these compounds. The n-hexane fraction did not showed significant antimicrobial activity against several bacterial strains, only Micrococcus luteus was inhibited by one of the non-polar fraction. However, the distinctive chemical composition of T. terrestris twigs indicate its potential as a source of natural antimicrobial compounds.

Enhancement of Phytochemical Analysis of Tribulus Terrestris Harvested in Tunisia and Marketed as Dietary-Supplement in Developed Countries

International Journal of Progressive Sciences and Technologies, 2018

The aim of the present study was to detect, quantify and identify the phenolic compounds in Tribulus terr remedy in infertility disorder by Ayurveda therapy) extract hydrolyzed by HCL, using high (HPLC) to detect and quantify non-volatiles compounds. Gas to detect and quantify volatile-compounds. A total of fifty (50), eighteen (18), and twenty (20) compounds f respectively in seeds, leaves and roots were identified as belonging to various structural classes, mainly phenolic compounds by HPLC finger print allowed the identification of procatechic Keywords-Dietary-Supplement; Nutraceutical I. INTRODUCTION Polyphenolics, which are widely distributed in plants, currently are among the most studied phytochemicals because of their perceptible chemical properties (Lall et al., 2015). Polyphenols, a large group of compounds ubiquitously expressed in plants constitute one of the most numerous and large groups of substances in the plant kingdom, currently with over 8000 kno structures published (Bravo, 1998). These compounds can be subdivided into various classes according to the number of their phenol rings and the structural elements linked to the basic units (Deshpande et al., 1984). A classification of the twenty one (21) principal structures based on the number of carbons in the molecule has been established (Deshpande et al., 1984).

Evaluation of antibiotic potential of alkaloids of Tribulus terrestris L. against some pathogenic microorganisms

International Journal of Green Pharmacy, 2013

Context: Antibiotic resistance has become a global concern. There has been an increasing incidence of multiple resistances in human pathogenic microorganisms in recent years, largely due to indiscriminate use of commercial antimicrobial drugs commonly employed in the treatment of infectious diseases. Tribulus terrestris (Family: Zygophyllaceae) is a well-known medicinal plant and has been used worldwide for the treatment of infectious diseases. Alkaloids are group of naturally occurring chemical compounds, which are known to have antimicrobial properties. Aim: The present study is designed to evaluate the antimicrobial potential of alkaloids of T. terrestris L. Materials and Methods: Alkaloids extracted from different parts (root, stem, leaf and fruits) of T. terrestris were screened by Disc diffusion assay against three bacterial strains: gram positive (Staphylococcus aureus), gram negative (Escherichia coli and Proteus mirabilis) and two fungal strains: Aspergillus flavus and Aspergillus niger. Minimum inhibitory concentration of the extracts was evaluated by micro broth dilution method while minimum bactericidal/fungicidal concentration was determined by sub culturing the relevant samples. Total activity of the extracts against each sensitive test pathogens was also calculated. Statistical Analysis: Mean value and standard error mean were calculated for the results of disc diffusion assay and data were analysed by one-way analysis of variance and P values were considered significant at P < 0.05. Results: The alkaloid extracts of T. terrestris showed significant antibacterial potential as all test extracts were found active against both the gram positive and negative bacteria, but were inactive against the tested fungi. Conclusion: The present study indicates the broad spectrum antibacterial potential of the alkaloids of T. terrestris, hence may be exploited for future antibacterial drugs.

Therapeutic, Physicochemical, and Pharmaceutical Properties of the Active Compounds Found in Tribulus Terrestris

Introduction: Tribulus terrestris is rich in bioactive compounds such as saponins, flavonoids, and alkaloids, which have been researched for their therapeutic properties. Its diuretic effects can help with hypertension and edema, its aphrodisiac properties can aid sexual function, and its antilithic properties can prevent kidney stone formation. The physicochemical and pharmacological characteristics of Tribulus terrestris are currently being studied. Methods and Materials: We utilized the PubChem database to gather data on the studied compounds, including their formulas and structures. Subsequently, we employed SwissADME software to assess the physicochemical and pharmacological properties of these compounds in Tribulus terrestris (TT). Results and Discussion: Steroidal saponins and sterols exhibit varying solubility levels in physiological fluids due to their hydrophobic nature and glycosylation patterns. Flavonoids, on the other hand, demonstrate favorable solubility. Compounds like (25R)-Spirosta-3,5-dien, β-sitosterol, and stigmasterol have low polarity, whereas Terrestrosin K, Terrestrosin F, Terrestroside B, Protodioscin, and Kaempferol-3-O-rutinoside can form more hydrogen bonds. Steroidal saponins are more readily absorbed in the gastrointestinal tract and can penetrate the blood-brain barrier. Kaempferol, chrysin, and quercetin show inhibitory effects on specific cytochrome P450 enzymes. β-sitosterol and stigmasterol have high rates of skin absorption. The bioavailability score ranges from 0.11 to 0.55, and synthetic accessibility scores are generally high. Conclusion: The steroidal saponins and flavonoids found in Tribulus terrestris show therapeutic potential for conditions such as hypertension, edema, and sexual dysfunction. Many of them possess favorable pharmaceutical properties for clinical use.

Bioactive Compounds, Antioxidant, Anti-Inflammatory, Anti-Cancer, and Toxicity Assessment of Tribulus terrestris—In Vitro and In Vivo Studies

Antioxidants

Tribulus terrestris L. belongs to the family Zygophyllaceae and integral part of various ancient medicinal systems including Chinese, Indian, and European to combat various health ailments. The aim of the present study was to assess the phytochemical constituents, in vitro antioxidant activity using DPPH, FRAP, and H2O2 assays, in vitro anticancer activity using MTT assay, and in vitro and in vivo anti-inflammatory properties of T. terrestris. The acute and sub-acute toxicity of extracts exhibiting most biological potential was examined using murine models. Liquid–liquid partitioning followed by RP–HPLC sub-fraction of crude extract was performed. After that, ESI-MS/MS analysis was done for the timid identification of bioactive metabolites responsible for bioactivities of sub-fractions and HPLC analysis to quantify the compounds using external standards. Among all extracts, T. terrestris methanol extract was noted to hold maximum phenolic (341.3 mg GAE/g) and flavonoid (209 mg QE/g)...

Study the Biological Activities of Tribulus Terrestris Extracts

waset.ac.nz

In this study the extracts of the Iraqi herb Tribulus terrestris (Al-Hassage or Al-Kutub) was done by using of polar and non polar solvents, then the biological activity of these extractants was studied in three fields, First, the antibacterial activity (in vitro) on gram positive bacteria (Staphylococcus aureus), and gram negative bacteria (E. coli, Proteus vulgaris, Pseudomonas aerugiuosa, and Klebsiella), all extracts showed considerable activity against all bacteria. Second, the effect of extracts on free serum testosterone level in male mice (in vivo), the alcoholic, and acetonitrilic extracts showed significant (P < 0.05) increase in free serum testosterone level, and we found that the extracts contained compounds with less genotoxic effects in mice germ cells. 3 rd , was to study the effect of methanolic extract of T. terrestris in diabetes management.

Tribulus terrestris - an Ethnomedical Phytochemical Review

Archives of Natural and Medicinal Chemistry, 2017

Tribulus terrestris has long been used for the treatment of various chronic and acute ailments. The aim of the current review is to compile literature encompassing all scientific aspects including: pharmacological properties, safety/toxicity studies, pharmacognostic studies and phytochemical investigation of this plant. The compiled data may be helpful for the researchers to focus on the priority areas of research yet to be discovered. Complete information about the plant has been collected from various books, journals and Ayurvedic classical texts.

Phytochemical and Antioxidant Investigation of Tribulus terrestris

Planta Medica, 2013

Background: Clausena anisata (Willd). Hook (Rutaceae), also known as Iperepesi in Xhosa language is a medicinal plant widely used by herbalists for the treatment and/or management of several ailments such as chronic cough, tuberculosis and lung ulceration in Eastern Cape, South Africa. With reference to the information gathered in our previous study, we investigated the plant's phyto-constituents, as well as its inhibitory effects using aqueous and two different organic solvent of extractions in order to justify its folkloric usage. Methods: Antioxidant activity of the plant was screened through 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzthiazoline-6sulfonic acid) (ABTS) diammonium salt, nitric oxide (NO), and ferric reducing power. Total phenols, flavonoids, flavonols, proanthocyanidins, tannins, alkaloids and saponins were investigated using spectroscopic techniques. Results: There were no significant differences in the flavonoid and proanthocyanidins contents between the leaves and bark extracts of C. anisata respectively, while the total phenolic content of the bark extract of C. anisata was significantly higher than that of the C. anisata leaf. The acetone extracts of both the leaf and bark indicated strong antioxidant activities. Conclusion: The observed activities of the plant extracts could be attributed to the high contents of the phenolics, alkaloids, flavonoids, saponins, proanthocyanidins and tannin. The acetone extracts of the plants have also exhibited strong antioxidant activities in vitro. It has been established scientifically that oxidative stress is linked with several degenerative conditions and diseases; the inhibitory effects of these plant extracts on the free radicals could logically justify the folkloric usage of C. anisata leaf and bark in the Eastern Cape for the treatment of respiratory infection diseases.