Whole-Genome Sequence Resource of Indian Race 4 of Xanthomonas campestris pv. campestris, the Causal Agent of Black Rot Disease of Brassica oleracea var. capitata (original) (raw)

Xanthomonas campestris pv. campestris is a causative agent of black rot disease of cruciferous crops. A whole-genome sequence of any race of X. campestris pv. campestris has not been reported from India. The isolate Xcc-C7, race 4, of X. campestris pv. campestris was isolated from cabbage (Brassica oleracea var. capitata) from Bengaluru, in the southern parts of India. Whole-genome sequence data were generated by the next-generation sequencingbased single-molecule real-time sequencing (SMRT) techniques. This study will improve our knowledge of genomic diversity in X. campestris pv. campestris and pave the way for research on host-pathogen interactions (crucifer crops-X. campestris pv. campestris) to develop resistance in cultivated Brassicaceae crops. X. campestris pv. campestris (Pammel) Dowson is a Gram-negative, rod-shaped pathogenic bacterium that causes black rot disease in many cruciferous crops, worldwide. It is a vascular disease developing 'V'-shaped yellow lesions accompanied by blackened veins at margin of the leaf (Fig. 1A). It is genetically diversified by the specific host range of crucifer crops, including B. oleracea vegetables (cole crops such as cabbage, cauliflower, brussels sprout, broccoli, knoll khol, and kale), oilseed crops, ornamentals, and weeds (Singh et al. 2016; Vicente et al. 2001). The annual worldwide spread of black rot disease limits the yield of cole crops under favorable conditions (Singh and Dhar 2011; Singh et al. 2014). The diversity within X. campestris pv. campestris is reported by several researchers (Jensen et al. 2010; Singh and Dhar 2011; Singh et al. 2016). In fact, the pathovar is subdivided into nine races based on host-pathogen interactions (Vicente et al. 2001). According to Singh et al. (2016), only races 1, 4, and 6 of X. campestris pv. campestris of crucifer crops have been identified in India, with races 1 and 4 occurring predominately worldwide. Races 2, 3, and 5 seem to be rare (