Reverse phase-high performance liquid chromatography-diode array detector (RP-HPLC-DAD) analysis of flavonoids profile from curry leaf (Murraya koenigii. L) (original) (raw)

Journal of medicinal plant research

Murraya koenigii L. Spreng, a medicinally important herb of Indian origin, has been used for centuries in the Ayurvedic system of traditional Indian medicine and popularly used in Indian cuisine on a daily basis. To evaluate the quality of M. koenigii, L. leaves, a sensitive, simple and precise reversed-phase high performance liquid chromatography (RP-HPLC) method was developed for assessment of four major bioactive flavonoids: rutin, quercetin, myricetin and kaempferol. Separation was achieved on a reversed phase column (ZORBAX, Eclipse plus-C18, 5 μm, 4.6 × 150 mm, Agilent, USA) and methanol:acetonitrile:water:acetic acid (40:20:39:1, v/v/v/v) was employed as the eluent. Sample was eluted at 0.8 ml/min and peaks were simultaneously identified using UV absorbance at 350 nm for kaempferol and 254 nm for rutin, myrcetin, and quercetin. The authenticated four analytes were used to construct linear standard curves by injecting range of 20 to 200 ng. The correlation coefficients of the ...

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