Bee Pollen Extracts: Chemical Composition, Antioxidant Properties, and Effect on the Growth of Selected Probiotic and Pathogenic Bacteria (original) (raw)
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Molecules, 2021
In this study, the botanical origin, total flavonoid and phenolic content, antioxidant activity, phenolic profile and fatty acid composition of mixed bee pollen loads collected in Bayburt, Turkey, were determined. In addition to these assays, antibacterial activity of bee-collected pollen extract (BCPE) against a variety of food-borne pathogenic bacteria was determined in vitro. Pollen loads were classified into five botanical families based on their color: Asteraceae, Fabaceae, Campanulaceae, Cistaceae and Rosaceae. Total flavonoid, total phenolic, CUPRAC and CERAC concentrations were 173.52 mg GAE/g, 79.21 mg QE/g, 85.59 mg Trolox/g and 118.13 mg Trolox/g, respectively. Twenty-three phenolic compounds were scanned in bee pollen extract by LC-MS/MS, with rutin being the most abundant. Cis-4,7,10,13,16,19 docosahexaenoic acid was the predominant fatty acid, followed by cis-11-eicosenoic acid, palmitic acid, and alfa linolenic acid. In addition, the agar well diffusion (AWD) and micr...
International Journal of Bioscience, Biochemistry and Bioinformatics, 2020
Bee pollen is a natural product from honeybees which is rich in bioactive compounds. Bee pollen grain and powder were extracted using conventional and sonication extraction methods. All bee pollen crude extracts could not inhibit growth of test pathogenic bacteria. The antioxidant activity was analyzed via DPPH free radical scavenging activity assay and found that the ethanolic extract of bee pollen grain obtained by conventional method had the highest antioxidant activities (p<0.05), 40.69±3.01 mg GAE/g extract. When phenolic content was evaluated, the aqueous extract of bee pollen grain by conventional method showed the highest total phenolic content (103.06±1.96 mg GAE/g extract) while ethanolic extract of bee pollen powder by conventional method exhibited the highest flavonoid content, 56.40±4.85 mg QE/g extract. The data obtained provides the guideline for insightful evaluation of bee pollen biological activities for designation of novel supplementary food products in the future.
Brazilian bee pollen: phenolic content, antioxidant properties and antimicrobial activity
Journal of Apicultural Research, 2020
Bee pollen has been promoted as a dietary supplement for humans due to its nutritional and bioactive properties. Sixty-two samples of Apis mellifera dehydrated bee pollen collected in Brazil (eight states and Federal District) were analyzed for phenolic compounds, flavonoids, antioxidant activity using DPPH, b-carotene and ORAC methods, and antimicrobial activity. The values obtained for total phenolic compounds ranged from 12.60 to 84.22 mg GAE/g bee pollen while total flavonoids oscillated between 1.90 and 36.85 mg quercetin/g bee pollen. The IC 50 ranged from 0.35 to 13.42 mg bee pollen/mL of extract. The inhibition percentages ranged from 52.58 to 98.37% of bee pollen extract using the b-carotene method. When measured by the ORAC method, antioxidant activity was between 132.98 and 575.85 lmols eq. Trolox/g bee pollen. Bee pollen efficiently inhibited the growth of all the microorganisms studied. Candida albicans was the most resistant, while Staphylococcus epidermides was the most sensitive.
Food and Chemical Toxicology, 2014
Bee pollen is considered, since memorable times, a good source of nourishing substances and energy. The present study aimed to evaluate the biological activities of eight commercial bee pollens purchased from the market. The origin of sample A was not specified in the labeling; samples B, C, D and G were from Portugal and the remaining were from Spain. The sample E presented the highest value of phenolics (32.15 ± 2.12 mg/g) and the H the lowest (18.55 ± 095 mg/g). Sample C had the highest value of flavonoids (10.14 ± 1.57 mg/g) and sample H the lowest (3.92 ± 0.68 mg/g). All the samples exhibited antimicrobial activity, being Staphylococcus aureus the most sensitive and Candida glabrata the most resistant of the microorganisms studied. All the samples exhibited antimutagenic activity, even though some samples were more effective in decreasing the number of gene conversion colonies and mutant colonies. Regarding the antioxidant activity, assessed using two methods, the more effective was sample B. The anti-inflammatory activity, assessed using the hyaluronidase enzyme, was highest in samples B and D. Pearson's correlation coefficients between polyphenols, flavonoids, antioxidant activity and antimicrobial activity were computed. It was also performed a discriminant analysis.
Study of preparations of bee pollen extracts, antioxidant and antibacterial activity
Ciencia E Agrotecnologia, 2007
The aim of this study was to determine the antioxidant activity, phenolic content and antibacterial activity of pollen extracts obtained with different concentrations of ethanol. Each extraction condition (ethanol solutions from 40 to 90%) had a different effect in the phenolic compounds content. Although, the pollen extract obtained at 60, 70 and 80% of ethanol showed relatively higher levels of phenolic compounds (>10 mg/g) and did not present statistical significant difference between the extraction conditions. The amount of total phenolics ranged from 3.6 to 8.1 and 6.6 to 10.9 mg GAE/g for Alagoas state and Parana state pollen, respectively. The higher value for antioxidant activity index was 83.30% for the pollen from Alagoas state and 81.15 % for Parana state pollen. The highest degree of antioxidant activity was found in the extraction at 60% of ethanol solution for Parana state pollen, which also showed the highest concentration of polyphenol compounds. Staphylococcus aureus was inhibited by the ethanolic extract of Alagoas state pollen in all the concentrations of solvent, except the ethanolic extract of pollen at 90%. The extract at 60% of ethanol solution (Parana sample) inhibited Bacillus subtilis, Pseudomonas aeruginosa and Klebsiella sp.
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 2021
Bee pollen has many therapeutic properties with its rich chemical content. Especially the phenolic substances in the structure of bee pollen are very effective in showing the bioactive compounds. Thanks to these properties, bee pollen is used as a food supplement. It is known that these features are influenced by many factors such as climate, geography and plant origin. Therefore, it is important to determine the botanical origins of bee products. In this study, botanical origins and total bioactive compounds were determined at the seven bee pollen samples collected from different regions of Turkey. Also, antimicrobial activity of the seven bee pollen samples against seven different bacterial pathogens and one fungal agent using broth microdilution method were studied. Asteraceae, Fabaceae, Cistaceae, Papaveraceae families were found as a dominant in the regions by botanical origins examination. Total phenolic values were found between 24.77 ± 288.824 mg GAE/g and 51.61 ± 727.14 mg GAE/g. Total flavonoid content was 1.36 ± 0.015 mg QE/g and 2.40 ± 0.077 mg QE/g. The antimicrobial results showed that each of the tested bee pollen samples inhibited the growth of the gram-positive and gramnegative bacteria at the different levels while they did not have a complete inhibitory effect on the fungi.
Organic bee pollen (BP, n = 22) harvested from the Douro International Natural Park (DINP, Portugal) was studied. Nine botanical families were found in the mixture of the samples. The water activity and pH ranged 0.21-0.37 and 4.3-5.2, respectively. The BP analyses averaged 67.7% carbohydrates, 21.8% crude protein, 5.2% crude fat and 2.9% ash. The energy ranged from 396.4 to 411.1 kcal/100 g. The principal fatty acid found was linolenic, followed by linoleic acid, palmitic acid and oleic acid. The phenolic and flavonoid contents varied from 12.9 to 19.8 mg of gallic acid equivalents/g of extract and from 4.5 to 7.1 mg of catechin equivalents/g of extract, respectively. The scavenger activity and β-carotene bleaching assays values (EC 50 ) were 3.0 ± 0.7 mg/mL and 4.6 mg/mL ± 0.9 mg/mL, respectively. E. coli, sulphite-reducing Clostridia, Salmonella and S. aureus were not found. Since there are studies indicating appreciable differences among BPs from different regions, the full characterization of BP from diverse origins still appears to be a sound research priority in order to obtain reliable data about this beehive product.
Chemical composition and microbiological quality of bee pollen
Scientia Agraria Paranaensis, 2020
Besides honey and propolis, bee products already well consolidated, pollen also has relevant economic, nutritional and functional value. As the quality of the final product is directly related to the region where it is collected and processed, this work has evaluated the nutritional and microbiological quality of two dehydrated bee pollens from São Paulo state and the other produced by beekeepers from northern of Mato Grosso state, but with no inspection. Physical-chemical and microbiological quality, phenolic composition and mineral profile analysis were performed. It was observed that both pollens presented satisfactory quality regarding protein contents (24.8 ± 2.4 g 100 g-1), total sugars (36.2 ± 1.1 g 100 g-1), lipids (4.0 ± 0.3 g 100 g-1), ashes (2.6 ± 0.05 g 100 g-1), free acidity (238.7 ± 4.5 mEq Kg-1) and pH (4.8 ± 0.03), however regarding the humidity levels (6.6 ± 2.2 g 100 g-1) both samples were not in accordance with recommended by law (4 g 100 g-1). The bioactive profi...
Journal of applied botany and food quality, 2017
Harvested bee pollen is valuable for its nutritional value and healthy properties. This work relates the botanical origin of sixteen bee pollens from Chile with their phenolic, protein and carotenoid content, and antioxidant/antibacterial activities. Our results showed that the chemical properties of different bee pollens are associated with the plant’ species from which each one was derived from. Some correlations between chemical properties and botanical origin were observed. Bee pollen showed between 20.0- 30.4% protein, 2.8-50.2 mg/kg carotenoids, 22.8-918.4 mg/kg phenolics, and 4.51-91.19 mmol Fe+2/kg pollen. Antibacterial activity was observed against all bacteria assayed even surpassing the activity of traditional antibiotics. Brassica sp. and Galega officinalis are an abundant source of antioxidants and antibacterial compounds. Other species such as those derived from fruit and endemic plants from Chile, although they occur less frequently, are also good source of these comp...