Amycolatopsis taiwanensis sp. nov., from soil (original) (raw)
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Amycolatopsis magusensis sp. nov., isolated from soil
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2013
A novel actinomycete, designated strain KT2025 T , was isolated from arid soil collected from Magusa, northern Cyprus. The taxonomic position of the novel strain was established by using a polyphasic approach. The organism had chemical and morphological features consistent with its classification in the genus Amycolatopsis. Phylogenetic analyses based on 16S rRNA gene sequences supported the classification of the isolate in the genus Amycolatopsis and showed that the organism formed a cluster with Amycolatopsis nigrescens CSC17-Ta-90 T , Amycolatopsis minnesotensis 32U-2 T , Amycolatopsis sacchari DSM 44468 T and Amycolatopsis dongchuanensis YIM 75904 T . 16S rRNA gene sequence similarity analysis indicated that strain KT2025 T was most closely related to Amycolatopsis lurida DSM 43134 T (97.5 %), Amycolatopsis keratiniphila subsp. keratiniphila DSM 44409 T (97.4 %), Amycolatopsis keratiniphila subsp. nogabecina DSM 44586 T (97.1 %), Amycolatopsis nigrescens DSM 44992 T (97.1 %), Amycolatopsis azurea DSM 43854 T (97.1 %) and Amycolatopsis minnesotensis DSM 44988 T (96.9 %). The organism was found to have chemical features typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars. The predominant menaquinone was MK-9(H 4 ). The polar lipids detected were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and hydroxy-phosphatidylethanolamine. The major fatty acids were iso-C 16 : 0 , iso-C 15 : 0 and iso-C 14 : 0 . The G+C content of the genomic DNA was 70.8 mol%. Phenotypic data clearly distinguished the isolate from its closest relatives. The combined genotypic and phenotypic data and low levels of DNA-DNA relatedness with its closest relatives indicated that strain KT2025 T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis magusensis sp. nov. is proposed. The type strain is KT2025 T (5DSM 45510 T 5KCTC 29056 T ).
Amycolatopsis minnesotensis sp. nov., isolated from a prairie soil
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2006
Two actinomycete strains, 32U-2 T and 32U-4, were isolated from a prairie soil in Minnesota and subjected to characterization by means of polyphasic taxonomy. The 16S rRNA gene sequences were determined following PCR amplification and cloning. A phylogenetic analysis, based on comparative analysis of 16S rRNA gene sequences, indicated that the organisms consistently formed a well-separated, distinct sub-branch within the radiation of the genus Amycolatopsis of the family Pseudonocardiaceae. The levels of 16S rRNA sequence similarity between the isolates and the type strains of recognized Amycolatopsis species ranged from 94?1 to 97?9 %. The highest levels of sequence similarity were found between the isolates and Amycolatopsis coloradensis (97?6-97?9 %), Amycolatopsis alba and Amycolatopsis orientalis (97?3-97?6 %) and Amycolatopsis lurida (97?2-97?5 %). Chemotaxonomic characteristics supported the phylogenetic relationships between the organisms and members of the genus Amycolatopsis. However, a broad range of phenotypic and genetic data revealed that the isolates should be classified as novel species of the genus Amycolatopsis, for which the name Amycolatopsis minnesotensis sp. nov. is proposed. The type strain is 32U-2 T (=KCCM 42246 T =NRRL B-24435 T).
2001
The taxonomic position of a group of moderately thermophilic actinomycetes isolated from vegetable matter was determined using a suite of genotypic and phenotypic properties. The organisms were found to share a range of chemical and morphological markers typical of members of the genus Amycolatopsis. A representative of the group, strain K24 T , formed a distinct phyletic line within the range of variation occupied by the genus Amycolatopsis in the 16S rDNA tree. The strains have many phenotypic properties in common and some of these distinguish the group from representatives of the validly described species of Amycolatopsis. It is clear from the combined datasets that the strains merit recognition as a new species of Amycolatopsis. The name proposed for the new species is Amycolatopsis sacchari ; the type strain is K24 T ( l DSM 44468 T l KCTC 9863 T ).
Amycolatopsis alba sp. nov., isolated from soil
International journal of systematic bacteriology, 1993
A new Amycolatopsis species isolated from soil produces a new glycopeptide antibiotic related to vancomycin. Traditional taxonomic methods and contemporary fatty acid analysis techniques were used to establish the position of this species. The hyphae fragment extensively when the organism is cultured in liquid media. The organism is characterized by white aerial hyphae that bear long chains of cylindrical conidia. The reverse side is yellowish brown; a faint light brown soluble pigment is occasionally produced. The organism has a type IV cell wall (meso-diaminopimelic acid), a type A whole-cell sugar pattern, and a type PII phospholipid pattern. Mycolic acids are not present in whole-cell hydrolysates. The major menaquinone is MK-9(H4); there is also a minor amount of MK-8(H4). The name proposed for this new species is Amycolatopsis alba. The type strain is strain A83850 (= NRRL 18532).
A New Species of Actinomycete, Amycolata alni
International Journal of Systematic Bacteriology, 1989
A new species of the genus Amycoluta, Amycolata alni, is proposed for strains which were mostly isolated from root nodules and rhizospheres of alder trees and were formerly assigned to the species Amycoluta uutotrophicu. Strains of this new species could be differentiated from representatives of A. uutotrophica by deoxyribonucleic acid homology data (17 to 29% relatedness), as well as by their ability to grow on salicin and D-gluconate, their failure to produce acid from cellobiose and meso-inositol, their resistance to penicillin and carbenicillin (10 pg/ml), and other characteristics. Phenotypic features and low levels of deoxyribonucleic acid homology distinguish A. alni from two other species of the genus Amycolatu, Amycoluta suturneu and Amycolata hydrocuibonoxydans. The type strain of A. alni is strain 3LS (= VKM Ac-901).
Amycolatopsis regifaucium sp. nov., a novel actinomycete that produces kigamicins
… of systematic and …, 2007
The taxonomic position of seven soil actinomycetes provisionally assigned to the genus Amycolatopsis was established in a polyphasic study. The isolates, which had identical 16S rRNA gene sequences, had closest similarity to the type strain of Amycolatopsis orientalis. A representative isolate, strain GY080 T , had chemotaxonomic properties that were typical of the genus Amycolatopsis and could be distinguished from the type strain of A. orientalis using DNA-DNA relatedness data. All of the isolates shared a phenotypic profile that distinguished them from representatives of phylogenetically closely related species. Amplified rDNA restriction analysis showed that the isolates formed a homogeneous group that was distinctly separate from single-membered groups consisting of representative Amycolatopsis type strains, including that of A. orientalis. Based on the combined genotypic and phenotypic evidence, it is proposed that the seven isolates be classified as representatives of a novel species for which the name Amycolatopsis regifaucium sp. nov. is proposed. The type strain is GY080 T (5DSM 45072 T 5NCIMB 14277 T).
Amycolatopsis rhabdoformis sp. nov., an actinomycete isolated from a tropical forest soil in Brazil
International journal of systematic and evolutionary microbiology, 2015
Strain SB026 T was isolated from Brazilian rainforest soil and its taxonomic position established using data from a polyphasic study. The organism showed a combination of chemotaxonomic and morphological features consistent with its classification in the genus Amycolatopsis and formed a branch in the Amycolatopsis 16S rRNA gene tree together with Amycolatopsis bullii NRRL B-24847 T , Amycolatopsis plumensis NRRL B-24324 T , Amycolatopsis tolypomycina DSM 44544 T and Amycolatopsis vancoresmycina NRRL B-24208 T . It was related most closely to A. bullii NRRL B-24847 T (99.0 % 16S rRNA gene sequence similarity), but was distinguished from this strain by a low level of DNA-DNA relatedness (~46 %) and discriminatory phenotypic properties.
The Journal of Antibiotics, 2014
Two novel members of the genus Amycolatopsis were isolated from soil samples collected in South Africa. Strains JS72 T and M29 T clustered in the same clade in the 16S-rRNA, gyrB-16S-rRNA and gyrB-recN gene trees. Both strains showed anti-mycobacterial activity. The oxyB P450 monooxygenase B gene required for the production of glycopeptide antibiotics was detected in both strains, while strain JS72 T was also shown to contain the 3-amino-5-hydroxy-benzoic acid synthase gene, which is required for the production of the ansamycin class of antibiotics. Genetic distance values (based on the gyrB and recN genes) were calculated between strains JS72 T and M29 T and their closest phylogenetic relatives. The values for strain JS72 T were all above the threshold values of 0.02 and 0.04, respectively, that have been proposed to distinguish Amycolatopsis-type strains. The gyrB-based values for strain M29 T were above the threshold for all but one strain; the recN-based values were all above the threshold. These data, along with DNA-DNA hybridization data, showed that strains JS72 T and M29 T belong to distinct genomic species. The physiological, phylogenetic and genetic distance data support the description of strains JS72 T and M29 T as the type strains of novel species, for which the names Amycolatopsis speibonae sp. nov. (¼ DSM 46660 T ¼ NRRL B-24958 T) and Amycolatopsis roodepoortensis sp. nov. (¼ DSM 46661 T ¼ NRRL B-24959 T) are proposed, respectively.