Differential expressions of cancer-associated genes and their regulatory miRNAs in colorectal carcinoma (original) (raw)

Colorectal cancer is one of the frequently seen malignancies in the world. To date, several oncogenes and tumor 26 suppressor genes have been identified and linked to colorectal cancer pathogenesis. Although recent advances in 27 the diagnosis and therapy of colorectal cancer are promising, identifying novel genetic contributors is still high 28 priority. In the present study, expression profile of some cancer-related genes and their regulatory miRNA mol-29 ecules were evaluated by using a high-throughput real-time PCR method. For the study, a total of 54 patients di-30 agnosed with CRC and normal colon tissue samples of 42 healthy controls were included. For the expression 31 analysis, total RNA was extracted from FFPE tissue samples and converted to cDNA. All expression analyses 32 were assessed by using Fluidigm Microfluidic Dynamic Array chips for 96 samples and the reactions were held 33 in Fluidigm BioMark™ HD System Real-Time PCR. As a result of the study, expression of the ADAMTS1, FHIT, 34 RUNX1, RUNX3 and WWOX genes was shown to be significantly altered in CRC tissues in contrast to normal tis-35 sue samples. Moreover, miR-378a-3p, miR-155-5p, miR-193b-3p, miR-96-5p, miR-17-5p, miR-27a-3p, miR-36 133b, miR-203a, miR-205-5p, miR-34c-5p, miR-130a-3p, miR-301a-3p, miR-132-3p, miR-222-3p, miR-34a-5p, 37 miR-21-5p, miR-29a-3p and miR-29b-3p were found to be significantly deregulated in CRC. Consequently, re-38 sults of the current study strongly suggest the involvement of novel cancer-related genes and their regulatory 39 miRNA in CRC physiopathology.