Probiotic capability of novel lactic acid bacteria isolated from worker honey bees gut microbiota (original) (raw)
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Lactic Acid Bacteria from Honey Bees Digestive Tract and Their Potential as Probiotics
Proceedings of the International Conference and the 10th Congress of the Entomological Society of Indonesia (ICCESI 2019), 2020
Honey bees (Apis spp.) are social insects that possess unique gut microbiota community. There are three main phyla that dominate the honey bees gut microbiota; Proteobacteria, Firmicutes, and Actinobacteria in which several species among them belong to the lactic acid bacteria (LAB). In general, some species of LAB can be developed as potential probiotics due to their capability to produce bioactive compounds such as organic acid (lactic acid, acetate acid and formic acid), hydrogen peroxide (H 2 O 2), ethanol, enzymes, benzoate, antimicrobial peptides (AMPs), free fatty acid, and volatile compounds that in a synergistic action work as broad-spectrum antimicrobials toward several pathogens. Several strains of LAB that living in symbiosis within the digestive tract of honey bees have been isolated, among them are quite promising to be developed as probiotics. Multiple studies have shown the inhibitory effect of LAB from honey bees against pathogenic microorganisms. Formic acid and lactic acid produced by symbiotic LAB could decrease the environmental pH of wounds, therefore prevent the growth of pathogenic microbes. Furthermore, the volatile compound produced by LAB is toxic, while H 2 O 2 in small numbers is needed for optimal wound recovery. LAB capable of producing broad-spectrum antibacterial compounds such as Serratia marcescens, Eschericia coli, methicillin-resistant Staphylococcus aureus, Klebsiella aerogenes, Salmonella typhi, Pseudomonas spp., Klebsiella spp., Proteus spp., S. aureus, and Bacillus subtilis. Additionally, LAB possess antioxidant activity and to adhere the epithelial cells. This paper aimed to discuss varieties of LAB from the digestive tract of honey bees and their potential as probiotics that could benefit healthcare.
Journal of Advances in Microbiology, 2018
The objective of this work was to isolate, identify and characterize lactobacilli strains from the intestinal tract of honey bees as putative probiotics. We obtained eighty-five isolates. At the end of screening based on physiological properties, 17 isolates were pre-selected and their resistance to gastrointestinal stress was evaluated. Twelve (12) with good resistance after 3 h exposure to low pH values (pH2, pH3) were subjected to determination of their in vitro BSH activity. The research of the bsh-A, bsh-B, Bsh1 and Bsh-Lp1 genes encoding the BSH enzyme was also conducted. Four isolates (H46, H82, H21 and H28) were resistant, seven others tolerant (H6, H15, H47, H24, H67, H44, H80) and only one was sensitive (H63) to oxgall bile salt. Determination of BSH activity revealed that all strains hydrolyze bile salts, with a preference for oxgall as opposed to Original Research Article
International journal of health sciences
Lactic acid bacteria (LAB) are known as the major group of probiotics that possess different beneficial properties. Several studies had reported the isolation of LAB from stingless bees. However, the isolation of LAB specifically from stingless bee gut is still limited. The bee microbiome frequently hosts LAB which demonstrated beneficial effects such as reducing bacterial and parasite infections as well as increasing honey output in beehives. This study aimed to characterize the probiotic properties and antimicrobial activity of LAB from stingless bee gut. A total of five LAB strains were successfully isolated from three different species of stingless bee known as Heterotrigona itama (HIT), Geniotrigona thoracica (GTH), and Tetragonula laeviceps (TLA). All LAB isolates were assessed for in vitro probiotic activity such as acid tolerance, bile salt tolerance, tolerance to simulated gastric juice, surface hydrophobicity, and also their antimicrobial activity. The percentage viability...
Probiotic potency of Lactobacillus plantarum KX519413 and KX519414 isolated from honey bee gut
FEMS microbiology letters, 2018
The Indian honey bee Apis cerana indica, which harbors an abundant and diverse range of lactic acid bacteria (LAB) in their gut with beneficial effects, was used as the source for the isolation of LAB. In the present study, two LAB isolates from honey bee gut were selected primarily based on their phenotypic and selective biochemical characterization, followed by PCR and identified using 16S rRNA sequencing as Lactobacillus plantarum and were registered in National Centre for Biotechnology Information under accession number KX519413 and KX519414. The probiotic potency of test strains indicated their survivability at acidic pH, bile salts and viability in simulated gastric juice enabling them to withstand gastrointestinal tract conditions. Evaluation of cell surface properties suggested that they possess an important defense mechanism against the pathogen since they are hydrophobic, auto-aggregative and have co-aggregative ability. Further, efficient exopolysaccharide production by t...
International Journal of Food Properties
Stingless bees harbor lactic acid bacteria (LAB) which possesses multiple beneficial properties. However, there is no report on LAB in stingless bee from Sabah and their products. This study aimed to isolate LAB from several stingless bee species and their products as well as to perform characterization and safety assessments. A total of 104 strains were isolated and seven potential antimicrobial LAB isolates were identified from stingless bee and their products. Characterization, identification, and assessments were performed on seven (A2b, B3b, P1b, H4b, A6, B5, and B10) LAB that exert potential antimicrobial activity against Listeria monocytogenes ATCC 7644. The A6 isolate was closely related to the Lactobacillus pentosus species whereas isolates B5 and B10 were closely related to Weissella paramesenteroides species. Finally, isolates P1b, H4b, B3b, and A2b were closely related to the Enterococcus sp. These seven LAB isolates were able to survive in stimulated gastrointestinal tract conditions (acidic, salt, bile salt, and temperature). The carbon fermentation, proteolytic activities, acidification, milk coagulation, and wide antibiotic susceptibility testing of seven LAB isolates revealed their potential used as a probiotic and fermentation purposes. Therefore, more studies are warranted to investigate the potential of these LAB isolates towards applications in probiotic and fermentation.
Journal of Applied Microbiology
Lactic acid bacteria (LAB) are widely distributed in nature and, due to their beneficial effects on the host, are used as probiotics. This review describes the applications of LAB in animal production systems such as beekeeping, poultry, swine and bovine production, particularly as probiotics used to improve health, enhance growth and reproductive performance. Given the importance of honeybees in nature and the beekeeping industry as a producer of healthy food worldwide, the focus of this review is on the coexistence of LAB with honeybees, their food and environment. The main LAB species isolated from the beehive and their potential technological use are described. Evidence is provided that 43 LAB bacteria species have been isolated from beehives, of which 20 showed inhibition against 28 species of human and animal pathogens, some of which are resistant to antibiotics. Additionally, the presence of LAB in the beehive and their relationship with antibacterial properties of honey and pollen is discussed. Finally, we describe the use of lactic bacteria from bee colonies and their antimicrobial effect against foodborne pathogens and human health. This review broadens knowledge by highlighting the importance of honeybee colonies as suppliers of LAB and functional food.
Cells
Numerous honeybee (Apis mellifera) products, such as honey, propolis, and bee venom, are used in traditional medicine to prevent illness and promote healing. Therefore, this insect has a huge impact on humans’ way of life and the environment. While the population of A. mellifera is large, there is concern that widespread commercialization of beekeeping, combined with environmental pollution and the action of bee pathogens, has caused significant problems for the health of honeybee populations. One of the strategies to preserve the welfare of honeybees is to better understand and protect their natural microbiota. This paper provides a unique overview of the latest research on the features and functioning of A. mellifera. Honeybee microbiome analysis focuses on both the function and numerous factors affecting it. In addition, we present the characteristics of lactic acid bacteria (LAB) as an important part of the gut community and their special beneficial activities for honeybee healt...
Biodiversitas Journal of Biological Diversity
This study aimed to isolate probiotic lactic acid bacteria (LAB) from stingless bee honey (Galo-galo) and apply it as a starter culture. This research used stingless bee honey (Galo-galo) from west Sumatra, Indonesia. There are 6 types of honey bees, namely Tetragonula sarawakensis (TS), Tetragonula testaceitarsis (TT), Tetragonula minangkabau (MK), Tetrigona binghami (TB), Geniotrigona thoracica (TR) and Heterotrigona itama (IT). The screening of probiotics was evaluated as the LAB isolates' ability to resist acid (pH 3), bile salts (0xgall 0.5%), and antimicrobial activity against pathogenic bacteria. Based on the probiotic selection, the TB1 isolate from Tetrigona binghami honey had an acid tolerance of 82.75%, bile salt tolerance of 94.44%, and the highest antimicrobial activity against pathogenic bacteria with more excellent resistance than antibiotics. The identification with 16S rRNA showed that the TB1 bacterial isolate had 100% similarity with Lactobacillus plantarum SN13TFurthermore, starter culture inoculated with 6% L.plantarum SN13T met the starter culture parameters, with a pH of 4.7, titratable acidity of 1.46%, and lactic acid bacteria counts of 9.0 x 10 9 CFU/mL. L.plantarum SN13T is a candidate probiotic bacteria isolated from the stingless bee honey (Tetrigona binghami). This bacteria has the potential to be developed as a starter for fermented milk processing in the food industry.
Pathogens
Lactic acid bacteria (LAB) are an essential part of the microbiota of the digestive tract of honeybees (Apis mellifera L.). Antagonistic activity of 103 LAB strains (isolates from different environments) against 21 honeybee pathogens/opportunistic pathogens (with agar slab method) was screened. The growth of Paenibacillus genus was inhibited to the most extent. The highest antagonistic activity was demonstrated by Lacticaseibacillus casei 12AN, while the lowest by Apilactobacillus kunkeei DSM 12361, a species naturally inhabiting the honeybee gut. LAB isolated from the honeybee environment demonstrated stronger antagonism against pathogens than collection strains. The antagonistic activity of cell-free supernatants (CFSs) from 24 LAB strains against 7 honeybee pathogens was additionally assessed at physiological pH with the microtitration method. The same was determined for selected CFSs at neutralized pH. CFSs with physiological pH showed significantly stronger antibacterial activi...
Properties of different lactic acid bacteria isolated from Apis mellifera L. bee-gut
Microbiological Research, 2011
Eight strains belonging to Lactobacillus spp. and five to Enterococcus spp. were isolated from the gut of worker Apis mellifera L. bees. Studies based on 16S rRNA sequencing revealed that AJ5, IG9, A15 and CRL1647 strains had a 99% identity with Lactobacillus johnsonii, while SM21 showed a 99% similarity with Enterococcus faecium. L. johnsonii CRL1647, AJ5 and IG9 were high lactic acid producers (values were between 177 and 275 mM), and in vitro they inhibited different human food-borne pathogens and Paenibacillus larvae, the American foulbrood agent. This bacterium was the most sensitive to the lactic acid effect being inhibited by 44 mM of this metabolite. L. johnsonii CRL1647, AJ5 and IG9 also presented important surface properties. These cells showed between 77% and 93% of auto-aggregation. The preliminary study of the chemical nature of the aggregating factors revealed that the molecules involved in the surface of each L. johnsonii strain were quite complex; and something of a peptidic nature was mainly involved. E. faecium SM21 produced bacteriocin-like compounds with anti-Listeria effects. Furthermore, a band close to 6.0–7.5 kDA was detected by SDS-PAGE studies, and the entA, B and P structural genes were amplified by PCR reactions. For the first time, bee-gut associated L. johnsonii and E. faecium strains have been isolated, identified, cultivated and some of their functional properties reported.