Antibacterial Activity of Romanian Propolis against Staphylococcus aureus Isolated from Dogs with Superficial Pyoderma: In Vitro Test (original) (raw)
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Antimicrobial activity of Argentinean propolis against Staphylococcus isolated of canine otitis
2014
Increasing resistance toward the available antibiotics by various microbial diseases pushed veterinarian to think about alternative therapies. Propolis is a natural compound well known since ancient times for its therapeutic effects and has been employed in traditional veterinary medicine. The aim of this study was to analyze the chemical composition of 12 propolis ethanolic extracts (PEEs) from different provinces of Northern Argentina and evaluate their antimicrobial activity against Staphylococcus strains isolated from canine otitis. Thirteen compounds (eight flavonoids, two chalcones and three phenolic acids) were identified by TLC, spectrophotometric methods and HPLC-DAD. PEEs were effective against Staphylococcus strains. The highest antibacterial activities were found in samples from Tucuman province. All propolis samples were considered bacteriostatic and the selection of resistant phenotypes did not occur in presence of Argentinean-PEE. Results of the present study indicate...
Journal of the Selva Andina Research Society, 2022
The objective of this research was based on the comparison of inhibition halos of a sample of Staphylococcus aureus, faced in vitro with eight antibiotics used in medicine, as well as four alcoholic concentrations of propolis. The results indicate that the antimicrobial action of propolis, depending on the bioactive compounds such as: flavonoids, polyphenols, aromatic acids, etc., contained in this product. The antibacterial activity of the four propolis dilutions produced inhibition halos between 10 and 20 mm. Of the eight antibiotics with which the comparison was made, only erythromycin was found to be resistant to S. aureus. Penicillin produced halos with smaller dimensions. Although the halos derived from the rest of the antimicrobials were greater than those from propolis, many of them were found within the range originated by said product from the hive. Compared antibacterial resistance has not been demonstrated, with which over the year's antibiotics present, when used indiscriminately to eliminate or control antimicrobial agents 2022. Journal of the Selva Andina Research Society ®. Bolivia. All rights reserved.
Advances in Animal and Veterinary Sciences, 2021
T he scientific community still busy lately in searching for natural plant and animal preparations for the purpose of using them in the treatment of bacterial, fungal and viral infections to get rid of the problems of adverse effects of drugs and reduce the resistance of organisms. Propolis is the common name of a combination of resinous compounds obtained in the northern temperature region by honeybees from sections of trees, buds and exudates, ranging from the chestnut are the primary origins of propolis (Wael et al., 2015). In the hive, propolis is typically used to cover the inner walls, to shield intruders, such as lizards and snakes, or even against wind and rain, and to discourage the growing of fungi and bacteria (Zhang, et al., 2009). It also forms phytochemical components proven to support differentiation or apoptosis in cells (Martinotti S and Ranzato E, 2014) Caffeic acid, caffeic phenyl ester, artepillin C, Quercetin, resveratrol, galangin, and genistein are among other Propolis, along with other honeybee commodities has potent therapeutic effects and has been used in overall prevalence in different countries since ancient times (Kuropatnicki et al., 2013). It is thought that propolis has antiseptic, antibacterial, antimycotic, astringent, spasmolytic, anti-inflammatory, anesthetic, antioxidant, antifungal, anti-ulcer, anticancer and immunomodulatory research Article Abstract | Objective: The main objective of this work was to measure the impact of synthetic and natural propolis on infected wounds contaminated with pathogenic staphylococcus. aureus. Material and methods: Sixteen healthy local rabbits were classified into two similar group. All rabbits were anaesthetized by 2% lidocaine hydrochloride S/c as local anesthetic, then surgical incision was performed 3*4cm in the skin. From one day of wound infected with staph. Aureus, first 8 rabbits treated with 5% natural propolis while the animals from (9-16) treated with synthetic propolis, Results: Temperature, respiratory rate and heart rate were included in clinical criteria investigation. there were no significant (p ≤0.05) differences between groups. Macroscopic finding showed that the scar present greater quantity in the animals exposed to synthetic propolis than that in group one furthermore the histopathological findings exhibited that the wound healing in the treated group by natural propolis was quicker and better and the epithelial cells with more maturity so the skin back up near normal, while the synthetic propolis group showed early epithelization with mild regeneration of adnexa. Conclusions: The healing properties of propolis very encouraging to make more trials in different species and types of tissues furthermore to analysis of constituent of propolis by accurate methods.
Study the bactericidal activity of Propolis in rabbit model
Journal of Physics: Conference Series, 2019
This study aims to assess the bactericidal activity of Propolis extract against methicillin resistance Staphylococcus aureus (MRSA) in vivo. It included (11 male white New Zealand rabbits) evenly divided into two groups (6 animals in each one). Group I was fed daily with one milliliter of 20% extract Propolis for twenty days, while group II was fed without Propolis to serve as a control group. Ten days after the last dose, all animals were injected in intraperitoneal with single dose viable MRSA antigen at 0.5 x108 cfu/ml. After two days, blood samples that collected from all animals by heart puncture were cultured on blood agar media to assess bactericidal power activity of extract Propolis. The results pointed to effect of this extract on survive viable MRSA bacteria as compared with survive of same bacteria in control group animals. It was concluded Propolis had bactericidal power against pathogenic bacteria that appear resistant to antibiotics like MRSA bacteria.
Effect of propolis extracts against methicillin-resistant Staphylococcus aureus
Main Group Chemistry, 2015
Staphylococcus aureus is a human pathogen that causes a wide range of infections worldwide. In this study, 40 clinical isolates of S. aureus were tested for their susceptibility to oxacillin and to different classes of antibiotics by disc diffusion method. Data revealed that out of these 40 isolates, 28 were MRSA. Most isolates were not only resistant to oxacillin but also found resistant to a number of other antibiotics. Propolis is a mixture of beeswax and resins collected by the honeybee from plant buds, leaves, and exudates. It has been used for centuries in folk medicine. The antimicrobial activity of ethanolic extract of propolis alone or in combination with antimicrobial drugs against MRSA isolates was evaluated. The results showed that the propolis extract was found to exhibit antimicrobial activity against all tested isolates. Also the results demonstrated the combination between propolis and some tested antimicrobial drugs in vitro is effective against multi-drug resistance MRSA.
Veterinary Microbiology, 2010
The aims of this study were to evaluate the antimicrobial potential of propolis extract by determining the minimum bactericidal concentration (MBC) for coagulase-positive Staphylococcus isolates (Staphylococcus aureus and Staphylococcus intermedius) and the minimum fungicidal concentration (MFC) for Malassezia pachydermatis isolates. The microorganisms were assayed using broth microdilution techniques. The MBC90 was 21 mg mL−1, and the MFC90 was 5.3 mg mL−1. The propolis extract was found to exhibit antimicrobial activity against both pathogens.
Multifactorial aspects of antimicrobial activity of propolis
Microbiological Research, 2006
We investigated the antibacterial activity of sub-inhibitory concentrations of ethanolic extract of propolis (EEP), and its effect on the antibacterial activity of some antibiotics. Some clinically isolated Gram-positive strains were used.
Propolis obtained by means of alkaline hydrolysis and action on Staphylococcus aureus
2009
The Brazilian propolis is investigated for its antibacterial properties. The aim of this work was to establish the extraction and purification method to display the antibacterial activity. Propolis of Apis mellifera was obtained from bee hives cultivated in Parana State, Brazil. In this study, we used ATCC 25923 Staphylococcus aureus strains and 158 Staphylococcus aureus strains isolated from cows with clinical mastitis. Extraction and partial purification: Several fractions were obtained during the partial purification: a crude ethanolic extract of propolis, a resinous material in ethanolic solution and an alkaline hydrolysis water-soluble compounds solution. The results show that the alkaline hydrolysis water-soluble compounds solution had a minimal inhibitory concentration of approximately 155.46 g/mL for Staphylococcus aureus. In this work, we obtained an aqueous solution of organic compounds extracted from Apis mellifera propolis that bears physical-chemical and biological characteristics capable to inhibit the proliferation of Gram-positive bacteria.
International Journal of Scientific Research in Science, Engineering and Technology, 2019
Introduction: Staphylococcus aureus is a Gram-positive round shaped bacterium frequently found in the upper respiratory tract and on the skin. The emergence of antibiotic-resistant strains of S. aureus such as methicillin-resistant S. aureus (MRSA) is a worldwide problem in clinical medicine. It is resistant to various antibiotic medications. Propolis is (bee glue) a flavonoid-rich product of honey comb, derives from the Greek pro “before” and polis “city”, it exhibits antibacterial and anti-inflammatory properties which indicate that it can be an extremely powerful natural antibiotic and useful when fighting off upper respiratory infections. Materials and Methods: This was a descriptive cross-sectional study conducted at Soba University Hospital, Sudan. Following ethical consideration, 100 isolates of Staphylococcus aureus from different clinical samples, 50 samples of MSSA and 50 samples of MRSA were enrolled. subculture were used to re-identified the Staphylococcus aureus based on colonial morphology, Gram’s stain, and other biochemical test were used for MSSA and MRSA detection. Perforated plastic plate’s technique used for propolis collection and measurement of the inhibition zone were used for detection of sensitivy or resistant reaction. Results: All of the study MSSA samples (50/50; 100%) and MRSA (50/50; 100%) samples which cultured with a different concentrations of Al-Gelly propolis extract were shown a resistant inhibition zone while (50/50; 100%) of the study MSSA samples and (50/50; 100%) of the study MRSA samples which cultured with a different concentrations of Al-Fao propolis extract were shown sensitive inhibition zone at different sensitivity levels. The sensitivity levels of both MSSA and MRSA to the Al-Fao propolis extract was significantly correlate with the concentration of the propolis extract (P value 0.000, 0.000 respectively). The greatest effect will be a product of 20% concentration.