Studying the Impact of Golgi Glypican73 Serving as a Candidate Biomarker in Early Diagnosis for Hepatocellular Carcinoma among Saudi Patients (original) (raw)
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Asian Pacific Journal of Cancer Prevention : APJCP, 2019
Background: Due to the prevalence of Hepatocellular carcinoma (HCC) in Saudi Arabia, using new markers to give best diagnostic performance than alpha-feto protein (AFP) are important in early diagnosis. The aim of this work was to compare the significance between serum and mRNA Golgi glypican73 (GP-73) as newly identified diagnostic and prognostic markers for HCC among Saudi patients. Materials and Methods: A total of 300 subjects were divided into: 250 blood samples where 145 samples from HCC, 105 samples from chronic liver cirrhosis (CLC) and 50 normal controls were investigated for serum GP73 (sGP73) by ELISA. GP-73 mRNA from peripheral blood mononuclear cells was amplified by RT-PCR. The sensitivity and specificity of both techniques was compared. Results: Serum Golgi glypican 73 was significantly higher in HCC group compared to cirrhotic and normal controls (p<0.001). Sensitivity and specificity were 95% for sGP-73, 100% and 90% for Golgi glypican 73 mRNA. The combination of...
International Journal of Current Advanced Research, 2017
In the search for serum markers of hepatocellular cancer, several studies try to find a noninvasive marker for diagnosis of hepatocellular carcinoma (HCC) to facilitate the diagnosis and avoid the harmful complications of liver biopsy. Investigators have focused on Golgi protein 73 (GP73); also known as Golgi membrane protein 1 (Golm1) or Golgi Phosphoprotein 2 (Golph2) as non-invasive, available and inexpensive marker for the diagnosis of HCC. The present study aimed to evaluate the serum GP-73 mRNA expression as a marker for HCC diagnosis among Egyptian patients versus alpha fetoprotein. Subjects and Methods: The patients selected from the Hepatology, Gastroenterology and Infectious Diseases Department and from Gastroenterology and Hepatology Unit of Internal Medicine Department, Benha University Hospital. Subjects were classified into three groups: Group I : Included 20 apparently healthy subjects. Group II: Included 20 cirrhotic patients and not complicated by HCC, Group III: Included 40 patients diagnosed as liver cirrhosis complicated by HCC. The serum Golgi Protein 73 (GP73) mRNA was determined by semiquantitative RT-PCR. Results: GP-73mRNA was highly significant higher in HCC patients in comparison to cirrhotic and the control group (P < 0.001). ROC for AFP revealed that the best cut-off value is (>48ng/ml), at this point the sensitivity was 90 %, specificity was 90% and AUC was 0.96. However the ROC for GP-73mRNA expression revealed that the best cut-off value is (>5.11) at this point the sensitivity was 97.5%, specificity was 100% and AUC was 0.99. In conclusion, increased GP-73 mRNA expression could be associated with the presence of HCC and the serum Gp73 mRNA expression was more sensitive and more specific than AFP.
GP73, a resident Golgi glycoprotein, is a novel serum marker for hepatocellular carcinoma
Journal of Hepatology, 2005
Background/Aims: Golgi protein-73 (GP73) is up-regulated in hepatocellular carcinoma (HCC). The aims of this study were to determine if GP73 is detected in the serum, and to establish the sensitivity and specificity of serum GP73 for diagnosing HCC. Methods: Serum GP73 was detected by immunoblots and quantified by densitometric analysis. Results: A total of 352 patients were studied. Serum GP73 levels were significantly higher in patients with HCC compared to those with cirrhosis (P!0.001). GP73 had a sensitivity of 69% and a specificity of 75% at the optimal cutoff point of 10 relative units, with an area under the receiver operating curve of 0.79 vs. 0.61 for AFP (PZ0.001). GP73 levels had significantly higher sensitivity (62%) than AFP (25%) for diagnosing early HCC (P!0.0001). Moreover, GP73 levels were elevated in the serum of 57% (32/56) of individuals with HCC who had serum AFP levels less than 20 ng/ml. Conclusions: Higher levels of GP73 can be found in the serum of patients with HCC than of those without. GP73 was better than AFP for the diagnosis of early HCC. Further validation studies are needed to confirm the role of GP73 in the early detection of HCC.
The Egyptian Journal of Internal Medicine, 2017
Background and aim Hepatocellular carcinoma (HCC) is the third cause of cancer-related mortality. α-fetoprotein (AFP) is not a highly sensitive marker for predicting HCC despite its high specificity. Serum Golgi protein-73 (sGP73) seems to be promising new marker. This study aimed at evaluating the role of sGP73 alone and in combination with AFP for diagnosing HCC. Patients and methods This study was conducted on 90 Egyptian patients who were equally divided into two groups. Group 1 included 45 patients with hepatitis C virus-related liver cirrhosis without clinical or radiological evidence of HCC, and group 2 included 45 patients diagnosed as having HCC by triphasic abdominal computed tomography. Serum AFP and GP73 were measured using enzyme-linked immunosorbent assay. Results A cutoff value greater than or equal to 40 ng/ml for AFP had a sensitivity of 51.1%, specificity of 97.8%, and area under the curve (AUC) of 0.802. sGP73 with a cutoff value greater than or equal to 1.4 ng/ml yielded a sensitivity of 75.6%, specificity of 97.8%, diagnostic accuracy of 86.7%, and AUC of 0.908. The combined use of AFP and sGp73 led to an increase in sensitivity to 84.4%, specificity to 95.6%, diagnostic accuracy to 90%, and AUC to 0.947. Conclusion The combined use of AFP and sGp73 could increase the sensitivity and specificity for HCC diagnosis.
Accuracy of Serum Golgi Protein 73 and Alpha Fetoprotein (AFP) to Diagnose Hepatocellular Carcinoma
https://www.ijrrjournal.com/IJRR\_Vol.8\_Issue.9\_Sep2021/IJRR-Abstract059.html, 2021
Background: Hepatocellular Carcinoma (HCC) is one of the most common malignancies in the liver. Modalities of diagnostic are often an obstacle in HCC surveillance. Alpha fetoprotein (AFP) is one of protein that often used in the diagnostic of HCC in chronic liver disease. Golgi protein 73 (GP73), is one of the candidate biomarkers in early diagnostic of HCC and found in biliary epithelial cells but rarely expressed by normal hepatocytes. Expression of GP73 was reported to be increased in a large number of malignancies. Aims of this study to evaluate differences in Golgi protein 73 serum (sGP73) and AFP in diagnosing hepatocellular carcinoma in patients with liver cirrhosis. Materials and Methods: This cross-sectional study was conducted at Haji Adam Malik Hospital in 2020. Serum level of GP73 and others biomarker was detected using enzymelike immunosorbent assay. Results: From 90 subjects, Liver cirrhosis and HCC group had significantly higher AFP than the control group. AFP was superior in determining HCC to GP73. At a cut off value of > 394.5.00 ng/mL, AFP yielded a sensitivity of 83.3% and specificity of 67%, for discriminating liver cirrhosis and HCC (AUC 0.84), while GP73 with cut off value of > 82.5 ng/mL, sensitivity of 70% and specificity of 57% (AUC 0.74). Conclusion: GP73 was significantly higher in HCC patients in comparison to non-HCC patients and healthy population. Compared with alpha fetoprotein, GP73 was superior in discriminating HCC in healthy population but inferior in group of liver cirrhosis.
Asian Pacific Journal of Cancer Prevention
Background: Hepatocellular carcinoma (HCC) accounts for more than 80% of primary liver cancers. Moreover, in the next 10 years, more than one million patients are expected to die from liver cancer as estimated by the World Health Organization. The aim of the present study is to evaluate the clinical utility of using Glypican (GPC3), Vascular endothelial growth factor (VEGF) and Golgi protein 73 (GP73) in serum by Enzyme-Linked Immunosorbent Assay (ELISA) and by Real-Time Polymerase Chain Reaction (RT-PCR), as diagnostic markers to differentiate HCC from cirrhotic liver disease. Methods: A total of 50 patients with histologically-proven HCC, 50 liver cirrhosis patients and 20 healthy volunteers as controls were enrolled in this study, blood samples were obtained from each patient. Expression of the studied biomarkers was evaluated by ELISA and Real-Time PCR. Results: Statistical analysis of RT-PCR results showed that the expression of GPC3, VEGF and GP73 in serum of patients with HCC was significant (P value < 0.001, 0.01, and < 0.001) respectively and increased when compared to the cirrhotic group. Furthermore, the serum protein levels of GPC3 and VEGF in HCC and cirrhotic patients were significant when compared to the control group. While no significance was found between HCC and cirrhotic group. The serum protein level of GP73 was significantly increased in HCC and cirrhosis groups compared to the control group (P value < 0.001). Moreover, a significant increase was evident in HCC group compared to cirrhotic group (P value < 0.001). The results of the present study showed that the combination of VEGF and GP73 could discriminate HCC from cirrhosis. Conclusion: GPC3, VEGF and GP73 are reliable biomarkers for diagnosis of HCC. The serum level of GP73 is a potential screening marker for discriminating HCC from liver cirrhosis.
SERUM GOLGI PROTEIN 73 IN PATIENTS WITH HEPATOCELLULAR CARCINOMA
Background: Hepatocellular carcinoma (HCC) is the sixth most common cancer worldwide. The diagnosis of HCC is mainly based on a combination of abdominal ultrasound and serum alpha fetoprotein (AFP) level. Serum AFP has low sensitivity, serum Golgi protein 73 (sGP73) is a novel and promising biomarker for detection of hepatocellular carcinoma (HCC). However, there are few reports on the predictive values levels of GP73 in diagnosis of HCC. We aimed to evaluate the clinical usefulness of serum GP73 in the diagnosis of HCC. Methods: The study was conducted on 80 patients included 20 patients with chronic hepatitis C (group I), 20 patients with liver cirrhosis (group II divided into two subgroups of equal number compensated and decompensated liver cirrhosis), 40 patients with HCC (group III) in addition to 10 apparently healthy control subjects (group IV). All patients in this study were subjected to full history taking, thorough clinical examination, radiological investigations, routine laboratory investigations and serum AFP in addition to serum GP73 assay by ELISA technique. Results: Assessment of the diagnostic performance of serum GP73 and serum AFP in the present study in diagnosis of HCC revealed that serum GP73 at cutoff of 11.1 ng/mL showed a diagnostic sensitivity of 82.5%, specificity 70%, positive predictive value 78.6%, negative predictive value 75% and the area under the curve (AUC) was 0.8025, Regarding serum AFP at cutoff 320ng/mL showed the diagnostic sensitivity of 79.41%, specificity 60 %, PPV 65.9%%, NPV 75 %, AUC 0.766, This proved the superiority of GP73 estimation over AFP assay in cases of HCC, Conclusion: Serum GP73 had an overall performance better than AFP in detection of HCC in patients with chronic HCV related liver disease so it can be used in diagnosis of HCC.
Medical Journal Armed Forces India, 2021
Background: Hepatocellular carcinoma (HCC) is the most common primary hepatic malignancy. Early detection of HCC is always a challenging task for physicians. Serum Golgi protein 73 (GP73) is considered a potential tumor marker for the detection of HCC. However, the diagnostic value of GP73 for the HCC diagnosis is still controversial. This research was designed to assess the diagnostic efficacy of GP73 as a diagnostic tool for HCC in cases with hepatitis C virus-related cirrhosis. Methods: Eighty-seven subjects were allocated into four different groups in this prospective research (HCC, liver cirrhosis, chronic hepatitis C, and healthy control group). Serum alpha-fetoprotein (AFP) and GP73 were tested for all subjects in the study. Detection of focal hepatic lesions was based on imaging by abdominal ultrasonography and triphasic computed tomography. Results: The cutoff values for GP73 and AFP were 534.5 ng/L and 32 ng/mL, respectively. The specificity of GP73 was 87%, and the sensitivity was 88%, while the specificity and sensitivity of AFP levels were 80% and 72%, respectively. The negative predictive value of GP73 was 87.5%, and the positive predictive value of GP73 was 84.6%, while the same parameters of AFP were 73.1% and 75%, respectively. Conclusion: Serum Golgi protein 73 could be a valuable biomarker and a useful diagnostic tool for the early diagnosis of HCC in cases of hepatitis C virus-related cirrhosis.
Can Glypican3 be diagnostic for early hepatocellular carcinoma among Egyptian patients?
Asian Pacific journal of cancer prevention : APJCP, 2013
Because of the high prevalence of hepatocellular carcinoma (HCC) in Egypt, new markers with better diagnostic performance than alpha-feto protein (AFP) are needed to help in early diagnosis. The aim of this work was to compare the clinical utility of both serum and mRNA glypican3 (GPC3) as probable diagnostic markers for HCC among Egyptian patients. A total of 60 subjects, including 40 with HCC, 10 with cirrhosis and 10 normal controls were analyzed for serum GPC3 (sGPC3) by ELISA. GPC-3 mRNA from circulating peripheral blood mononuclear cells was amplified by RT-PCR. Both markers were compared to some prognostic factors of HCC, and sensitivity of both techniques was compared. Serum glypican-3 and AFP were significantly higher in the HCC group compared to cirrhotic and normal controls (p<0.001). Sensitivity and specificity were (95% each) for sGlypican-3, (82.5% and 85%) for AFP, and (100% and 90%) for Glypican3 mRNA , and (80% and 95%) for double combination between sGPC3 and AF...
Biomedical and Pharmacology Journal, 2018
In Saudi Arabia AFP considered the main serum marker for diagnostic Hepatocellular carcinoma (HCC), due to the continuous detection of HCC in Saudi Arabia, using new biomarkers for early surveillance are essential to control in prevalence of HCC. The present study depend oncompare the significant between serum and mRNA Glypican-3 (GPC-3) as newly identified diagnostic and prognostic biomarkers for HCC between study cases.And combined sensitivity of AFP and GPC-3. Three hundred study cases, divided into: 250 blood samples were 145 samples from HCC , 105 samples from chronic liver cirrhosis (CLC) and 50normal controls were investigated for serum GPC-3 (sGPC-3) bySandwich ELISA. Glypican-3 mRNA from whole blood cells was detected by quantitative RT-PCR. The comparison between two techniques was by sensitivity and specificity. The results of sGPC-3showedhigher significant in HCC group than CLC and normal controls (p<0.001). sGPC-3 sensitivity was 95% and specificity was100%, while inGPC-3 mRNA were 100% and 94% respectively. The combination ofsensitivity between AFP and sGPC-3 was 80% and 95% respectively. The data demonstrated that, can depend on sGPC-3 and Glypican-3 mRNA as tumor biomarkers fordetection and surveillance of Hepatocellular carcinoma in Saudi patients. The sensitivity of Reverse Transcriptase-PCR is high accurate (100%) than estimating sGPC-3 by ELISA (95%).