Some Studies on Salmonella Enterica Associated with Diarrhea in Cattle (original) (raw)
2016, Alexandria Journal of Veterinary Sciences
Bovine salmonellosis is a worldwide bacterial d isease causing great animal losses, public health and economic problems. The present study was conducted on a total nu mber o f 160 animals (130 calves and 30 adult cattle) at Alexandria governorate either indiv idually or in farms. Out o f them, 85 calves and 15 adult cattle were suffering fro m diarrhea while the remaining calves and adult cattle were apparently clin ically healthy, rectal swabs and fresh fecal samp les were collected and examined bacterio logically for the presence of Salmonella spp. The results revealed the isolation of Salmonella organism fro m 11 (13%) and 1 (6.6%) diarrheic calves and adult cattle, respectively, on contrary, no Salmonella could be isolated from all apparently clin ically healthy animals. The 12 Salmonella isolates were examined for antimicrobial sensitivity using 10 antibiotic discs; all 12 (100%) isolates showed resistance to at least one antimicrobial, the highest resistance observed for erythromycin 12 (100%), tetracycline 8 (67%) and amp icillin 7 (58%) while the highest sensitivity was observed for streptomycin 10 (80%), gentamycin 9 (75%) and chloramphenico l 7 (58%). Conventional PCR performed on 10 Salmonella isolates for the presence of three virulence genes: invasion gene (invA), enterotoxin gene (stn) and plasmid encoded fimb riae gene (pefA); invA gene was detected in 7 (70%) isolates while stn gene was detected only in 2 (20%) isolates, but pefA gene could not be detected in all 10 Salmonella isolates. In a conclusion, Salmonella is one of the most important causes of diarrhea in calves and adult cattle, Salmonella isolates were resistant to most commonly used antibiotics resulting in diff icult ies in their treat ment and the application of PCR assay is important for rapid detection of virulence profile of Salmonella.
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