Pharmacognostical Studies on Acer campestre L. subsp. campestre (original) (raw)
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4. Antibacterial activity of ethanolic leaf.pdf
Antimicrobial compounds from herbal sources have good therapeutic potential. In this study, the antibacterial effects of ethanolic extraction of Cotoneaster nummularioides were evaluated for its effect on four food pathogenic bacteria (two gram-positive bacteria; Bacillus cereus (PTCC 1015), Staphylococcus aureus (PTCC 1431) and two gram-negative bacteria; Salmonella Entrica (PTCC 1709) and Escherichia coli (PTCC 1399). The agar disk diffusion method and micro-broth dilution methods were used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The extract of C. nummularioides showed strong antibacterial activity against two gram-positive micro-organisms tested with higher sensitivity for Bacillus cereus (inhibition zone was 11 mm for the concentration of400mg/ml). Other concentrations of extract didn’t show strong inhibition zone on bacterial strains. The MIC was 200 mg/ml for two gram-positive bacteria (Bacillus cereus and Staphylococcus aureus)and MBC was >400 mg/ml for all bacterial strains.
2018
This study serves to explore the chemical constituents of Azadirachta indica and Mangifera indica as a possible alternative sources of conventional antibiotics. Ethanolic extracts of Azadirachta indica and Mangifera indica stem bark were analysed for their chemical constituents. Phytochemical values for (Tannins, Oxalate, Phytate, Terpenoids, Trypsin inhibitor, Total Phenol, Total Carotenoids, Total Carotene Carotenes, Xanthophyll, Flavonoids, Alkaloids, Saponins and Antioxidant (DPPH Scanvenger) were revealed. The result were subjected to Studentized T-test as contained in SAS (1999). The result showed that all secondary metabolites analyzed were present in the bark of the two plant species studied but at different concentrations. The concentration of Tannin (1510.00 mg/kg), Oxalate (139.20 mg/kg), Phytate (15.55 mg/kg), Trypsin inhibitor (730.00 mg/kg), Flavonoids (78.50) and Saponins (17.71%) contents of Mango stem bark were found to be more than in Azadirachta indica. However, A...
2016
Acorus calamus Linn. is a herbaceous plant with rhizome that is indefinite branched, smooth, pinkish or pale green.The leaf and rhizome part of Acorus Calamus possess antibacterial activity. Ocimum kilimandscharicum is a short herb, native to India. In traditional medicine, it is used to treat various ailments including colds, coughs, abdominal pains, measles and diarrhoea.The present study was to study the basic properties of Acorus calamus and Ocimum kilimandscharicum leaf extracts and to formulate a simple syrup and studying its activity on pathogens.The aqueous extracts of the leaves were studied for their total protein, free phenolic and hydroxyl free radical scavenging activity.The extracts and prepared syrup were tested for their antimicrobial activity against P.aragenosa, B.cereus, E.coli and N.meningitis. Both the prepared formulations were evaluated for various parameters such as pH, appearance, colour, odour and taste. We found that both the extracts possessed excellent a...
The current study included the preparation of the aqueous and alcoholic leaves extract , Ziziphus (Ziziphus spina-christi) and Eucalyptus plant (Eucalyptus camaldulensis) and assessed its antimicrobial activity against reference strains of bacteria and mold. Through chemical compound detection Ziziphus leaves results showed that the aqueous extract and alcohol contain all effective compounds that have been detected except composite resins in aqueous extract and resins in the alcoholic extract. The Eucalyptus leaves aqueous and alcohol extract contained resins, tannins, phenols. It was tested the effectiveness of the inhibitory plant extract against five isolates of bacteria and five isolates of molds. We used a concentration of 50 and 100 mg/ml of each extract. A similar maximum zone of inhibition through the ethanol extract was obtained against both bacteria and mold as well as increased efficiency as dose dependent .
In Vitro Antimicrobial Activity of the Alcoholic Extract of Quercus brantii subsp. persica
Journal of Human Environment and Health Promotion, 2022
Background: Lately, consumption of natural preservatives to improve food products' shelf life has been the center of attention. Due to the approved health benefits of the oak fruit, its application in food industry as a preservative seems satisfying. Methods: Antimicrobial activity of the alcoholic extract of the oak fruit in concentrations of 200, 400, 600 and 800 mg/mL was investigated on Escherichia coli, Staphylococcus aureus, Salmonella typhi, Klebsiella pneumoniae, and Saccharomyces cerevisiae with well diffusion technique. In order to detect the minimum inhibitory concentration (MIC) and minimum bacterial concentration (MBC), macro-dilution broth and subculturing on solid culture media techniques were used. Results: The most growth prevention and inhibitory effect was observed in S. aureus, S. cerevisiae, K. pneumoniae, S. typhi, and E. coli, respectively. The most and the least antimicrobial activity of the oak fruit extract were observed in S. aureus (MIC=0.15 mg/mL and MBC = 0.313 mg/mL) and E. coli (MIC = 2.5 mg/mL and MBC=5 mg/mL), respectively. Conclusion: The alcoholic extract of Iranian oak fruit had antimicrobial activity, and its impact was more profound on gram-positive bacteria such as S. aureus and S. cerevisiae than gram-negative ones namely E. coli, K. pneumoniae, and S. typhi.
Antimicrobial and antioxidant activities of alcoholic extracts of Rumex dentatus L
This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution and sharing with colleagues. Other uses, including reproduction and distribution, or selling or licensing copies, or posting to personal, institutional or third party websites are prohibited. In most cases authors are permitted to post their version of the article (e.g. in Word or Tex form) to their personal website or institutional repository. Authors requiring further information regarding Elsevier's archiving and manuscript policies are encouraged to visit: http://www.elsevier.com/copyright a b s t r a c t In-vitro antimicrobial and antioxidant activities of various concentrations ranging from 150 to 500 mg/ml of alcoholic (methanol and ethanol) extracts of Rumex dentatus were analyzed on different clinical bacterial strains (Shigella flexneri, Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Staphylococcus aureus) and fungal strains (Aspergillus versicolor, Aspergillus flavus, Acremonium spp., Penicillium dimorphosporum, Candida albicans, Candida kruesie, Candida parapsilosis) using agar disk diffusion method and broth dilution method (MIC and MBC determination) for antimi-crobial activity and DPPH (1,1-diphenyl-2-picrylhydrazyl) assay, Riboflavin photo-oxidation assay, deoxyribose assay, lipid peroxidation assay for antioxidant activity. The extracts showed maximum inhibitory effect against K. pneumonia and P. aeruginosa with no activity against S. typhimurium from among the bacterial strains while as in case of the fungal strains the maximum effect was observed against C. albicans by both the extracts. MIC and MBC values determined for active fractions of the extracts against some bacterial strains (S. flexneri, K. pneumonia and E. coli) revealed that the test organisms were inhibited by all the extracts with methanol showing lower values of both MIC and MBC indicating it as a better antimicrobial agent. The antioxidant activity showed that the extracts exhibited scavenging effect in concentration-dependent manner on superoxide anion radicals and hydroxyl radicals leading to the conclusion that the plant has got a broad spectrum antimicrobial and antioxidant activity and could be a potential alternative for treating various diseases.