Distribution of HCV genotypes among risk groups in Serbia (original) (raw)
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Hepatitis C virus genotypes in Serbia and Montenegro: the prevalence and clinical significance
World journal of gastroenterology : WJG, 2007
To investigate the prevalence of hepatitis C virus (HCV) genotypes in Serbia and Montenegro and their influence on some clinical characteristics in patients with chronic HCV infection. A total of 164 patients was investigated. Complete history, route of infection, assessment of alcohol consumption, an abdominal ultrasound, standard biochemical tests and liver biopsy were done. Gene sequencing of 5' NTR type-specific PCR or commercial kits was performed for HCV genotyping and subtyping. The SPSS for Windows (version 10.0) was used for univariate regression analysis with further multivariate analysis. The genotypes 1, 2, 3, 4, 1b3a and 1b4 were present in 57.9%, 3.7%, 23.2%, 6.7%, 6.7% and 1.8% of the patients, respectively. The genotype 1 (mainly the subtype 1b) was found to be independent of age in subjects older than 40 years, high viral load, more severe necro-inflammatory activity, advanced stage of fibrosis, and absence of intravenous drug abuse. The genotype 3a was associat...
Prevalence of hepatitis C virus genotypes in four risk groups in the Republic of Macedonia
Journal of Viral Hepatitis
PURPOSE OF THE STUDY: The prevalence of hepatitis C virus (HCV) genotypes depends on geographic location (1). HCV genotyping is highly important for severity of infection assessment as well as for epidemiological investigations (2). The aim of our study was to determine the HCV genotype prevalence in four different risk groups (intravenous drug users (IDU), hemodialysis patients, patient undergoing transfusion and other risk factors (persons exposed to contaminant needles or sharps, medical or dental procedures, piercing, tattooing, etc.)) in the Republic of Macedonia and to evaluate its association with patient’s age, gender and mode of transmission. METHOD: Detection of HCV was done by using commercial assay (AMPLICOR HCV v2.0). HCV genotyping was performed with an ASO hybridization method using specific oligonucleotide probes for HCV genotypes 1-4 (3). SUMMARY OF RESULTS: A total of 613 HCV positive patients were investigated. The prevalence of HCV infection in the different risk...
Bosnian journal of basic medical sciences / Udruženje basičnih mediciniskih znanosti = Association of Basic Medical Sciences, 2009
Prevalence of hepatitis C virus (HCV) genotypes in Bosnia and Herzegovina (B&H) is an issue that is not sufficiently researched and there is a need for studies that would explore this in detail.The aim of this study was to determine the distribution of HCV genotypes in the group of patients with chronic hepatitis C and also in the group of first time blood donors that tested positive for anti HCV antibodies during the blood screening process. Our secondary goal was to compare the proportions of HCV genotypes between these two groups.We analyzed 75 blood samples of patients with confirmed chronic hepatitis C. We also analyzed 13/16082 blood samples of first time blood donors found to be HCV positive during the blood screening process. We also determined HCV genotype in HCV RNA positive samples.We have found that genotype 1b was more prevalent in chronic hepatitis C patients (52/75; 69,3%) than in first time blood donors (6/13; 46,1%), however this difference was not statistically sig...
Distribution of HCV Genotype among the Patients Treated for Chronic Hepatitis C in Albania
2016
Background: Distribution of Hepatitis C Virus [HCV] genotypes may be changed over time. Epidemiological studies on distribution models of HCV genotypes in Albanian population might assist for better treatment options and preventive strategies. Aims: This study was conducted to determine distribution HCV genotypes in Albania by gender and age among the patients treated for HCV. Methodology : In this cross-sectional study, between April 2006 and December 2015, 265 patients identified with hepatitis C and under treatment were included. Frequency of different genotypes among patients was assessed according to gender, age at the time of sampling. Results: HCV genotype 1b was the predominant genotype [56 %] followed by genotype 2a/2c [17 %] among the Albanian patients with chronic HCV .A higher prevalence of 1b genotype of HCV was observed among male patients and those younger. Conclusion: Genotype 1b is the most prevalent genotype of HCV among the Albanian patients with chronic HCV. The ...
Distribution of hepatitis C virus genotypes and its response to treatment in Pakistani patients
Saudi medical journal, 2008
Objective: Hepatitis C virus (HCV) genotyping is an important part of pre-treatment diagnostic algorithms as it guides the choice of therapeutic regimens. The aim of this study was to analyse the distribution of HCV genotypes in patients with chronic hepatitis C from Croatia in the period 2008-2015. Methods: The study enrolled 3,655 anti-HCV positive patients with available results of HCV genotyping from the three largest national HCV genotyping laboratories. Results: The majority of HCV-infected individuals enrolled in the study were male (70.7%). Analysis of age distribution in a subset of 2,164 individuals showed a mean age of 40.9 years (SD 11.77 years). Croatian patients were mostly infected with HCV genotype 1 (56.6%), followed by genotype 3 (37.3%), genotype 4 (4.2%) and genotype 2 (1.8%). Genotype 1 subtyping in a subset of 1,488 patients showed 54% (803/1,488) of 1b infections and 46% (685/1,488) of 1a infections. Percentages of genotype 1 were the highest in Central/Northwestern and Eastern Croatia and the lowest in the Central/Southern Adriatic Region. Genotype 3 was most frequently found in the Central/Southern Adriatic Region (49.1%) but represented only 17.5% of infections in Eastern Croatia (p < 0.001). Conclusions: The results of this nine-year retrospective analysis on the distribution of HCV genotypes and subtypes in 3,655 HCV-infected individuals from Croatia showed that the majority of infections can be attributed to genotypes 1 and 3 with absence of major changes in the molecular epidemiology of the two most frequent HCV genotypes infection in Croatia in the past 20 years.
Most common HCV genotypes in patients from north-eastern Croatia
Acta medica academica, 2014
The aims of this study were to determine the HCV-RNA viral load, genotype distribution, risk factors and symptoms of HCVRNA positive viral load in HCV antibody-positive patients from north-eastern Croatia. From January 2009 to December 2011, 203 HCV antibody- positive patients (130 men and 73 women; median age 44.5 years) were analyzed for HCV-RNA by the COBAS TaqMan HCV test and genotyped by the Linear Array HCV Genotyping test (both from Roche). All patients completed a structured questionnaire about risk factors and symptoms. The HCV-RNA percentage was 61.1% and was similar for men and women. The HCV-RNA viral load increased with age: while 55% of 20-50 year old patients were HCV-RNA positive, 73% of patients >50 years were positive (p=0.021). Genotype 1 was the most prevalent genotype (79.8%), followed by 3 (12.9%), 4 (6.5%), and 2 (0.8%); genotypes 5 and 6 were not determined. Patients with genotype 1 (median, 50 years) were older than patients with 3 (median, 33.5 years) or...
Mediterranean Journal of Infection Microbes and Antimicrobials
This study aimed to investigate the distribution of hepatitis C virus (HCV) genotype and its variability in certain sociodemographics in patients with chronic hepatitis. Materials and Methods: Anti-HCV was performed by chemiluminescent micro-particle immune assay (Abbott Architect i2000SR, Germany), and HCV-RNA viral load detection was applied with real-time reverse transcriptase polymerase chain reaction (RT-PCR) in the system (Cobas AmpliPrep-Cobas TaqMan, Roche, Germany). Genotype detection was performed with RT-PCR upon with RT-PCR method in the system of Abbott RT-HCV Genotype 2 (Abbott Laboratories, USA) and with Bosphore-HCV Genotyping KitV3 in the Montania 4896 device (Anatolia Diagnostics and Biotechnology Products, Turkey). Frequency and percentage dispersions of all data obtained from patient files and laboratory information system were evaluated through the Statistical Package for the Social Sciences statistics software program. Results: HCV-RNA was positive in 628 of 2,381 patients with anti-HCV positivity (26.4%), and genotypes of 319 of which were evaluated. Mean age of 319 patients was 51.6 (standard deviation: 16.1). The most frequent genotypes were 1b (61%), 3 (19%), and 1a (10%). Incidences of genotype 1b among all genotypes between the dates of 2015-2018, were found 34.7%, 29%, 15.5% and 20.7% respectively (p=0.001). Contagion sources were medical interventions, and 1b was the most frequent genotype. Genotype 3 was most common in patients with intravenous drug addiction. A total of 168 of 238 patients who were Turkish citizens were detected to have genotype 1b, whereas 28 of them had genotype 3 and 25 had genotype 1a. Seventy-eight (24.7%) of the 316 patients, whose genotypes were tested, were foreigners coming mostly from Georgia, Turkmenstan, and Syria respectively. The most frequent genotype of Georgian and Turkmenistanian was 1b and Syrian was both 1a and 4. Conclusion: This study shows the most frequent genotype to be 1b and its prevalence is statistically decreased over the years, whereae other genotypes (1a, 3, 4, 3a, 1a/3, 1b/3, c-k, 2/3, 1/4, 3/4, and 5) increased.
Turkish Journal of Medical Sciences, 2016
Background/aim: The most common hepatitis C virus (HCV) genotype in Turkey is genotype 1. However, there has not been a study about the distribution of HCV genotypes among intravenous drug users (IVDUs) in the Çukurova region of Turkey. This study was planned to understand if there is a difference between IVDUs and the normal population. Materials and methods: Between May 2010 and May 2014, anti-HCV positive IVDUs who applied to the 6 hospitals in the Çukurova region of Turkey were included in this study. Their HCV genotypes were studied. Results: Ninety-seven anti-HCV positive IVDUs were screened in terms of HCV RNA and genotype. Ten were excluded from the study because their HCV RNA results were negative. Fifty-one of the 87 patients (58.6%) had genotype 3. Genotype 2 was detected in 26 (29.9%) and genotype 1 was detected in 10 (11.5%) patients. Conclusion: HCV genotypes seem to be different between the normal population and IVDUs according to studies worldwide. Among IVDUs, we detected a dominance of genotype 3 and genotype 2, which is apparently different from the normal population. The reason for this difference can be simply explained by infection through shared needles. However, there may still be a different immunological response in IVDUs, the investigation of which may lead to further studies.
Intervirology, 2014
Hepatitis C virus (HCV) has emerged as a leading cause of chronic hepatitis, liver cirrhosis and hepatocellular carcinoma worldwide. The purpose of this study was to describe the distribution pattern of HCV genotypes in chronic hepatitis patients in the Campania region of southern Italy and estimate their association with risk factors and viral load. 404 consecutive HCV ribonucleic acid-positive patients were included in the study. HCV genotyping was carried out by the HCV line probe assay test and viral load estimation by the TaqMan real-time PCR system. The predominant genotype was 1 (63.6%), followed by genotype 2 (29.4%), 3 (6.2%) and 4 (0.8%). Subtype 1b was more frequent in females than in males. Conversely, genotype 3 was more frequent in males. No significant difference was observed in age distribution of HCV genotypes. Surgery and dental therapy were the most frequent risk factors for genotype 1 and intravenous drug abuse and tattooing for genotype 3. Patients with genotype...
Distribution of different hepatitis C virus genotypes in patients with hepatitis C virus infection
World Journal of Gastroenterology, 2010
AIM: To investigate the presence of mixed infection and discrepancy between hepatitis C virus (HCV) geno types in plasma, peripheral blood mononuclear cells (PBMCs), and liver biopsy specimens. METHODS: From September 2008 up to April 2009, 133 patients with chronic hepatitis C referred to Fir ouzgar Hospital for initiation of an antiviral therapy were recruited in the study. Five milliliters of peripheral blood was collected from each patient and liver biopsy was performed in those who gave consent or had indica tions. HCV genotyping was done using INNO-LiPA™ HCV Ⅱ in serum, PBMCs, and liver biopsy specimens and then confirmed by sequencing of 5'-UTR fragments. RESULTS: The mean age of patients was 30.3 ± 17.1 years. Multiple transfusion was seen in 124 (93.2%) of patients. Multiple HCV genotypes were found in 3 (2.3%) of 133 plasma samples, 9 (6.8%) of 133 PBMC samples, and 8 (18.2%) of 44 liver biopsy specimens. It is no table that the different genotypes found in PBMCs were not the same as those found in plasma and liver biopsy specimens. CONCLUSION: Our study shows that a significant pro portion of patients with chronic hepatitis C are affected by multiple HCV genotypes which may not be detect able only in serum of patients.