Research Article Quantification of Total Phenol, Flavonoid Contents and Antioxidant Activity of Some Selected Indian Medicinal Plants (original) (raw)
Related papers
2018
Antioxidant properties of medicinal plants of Kandhamal district of Odisha have not reported, while these plants such as Nyctanthes arbor-tristis L. (NA), Tinospora cordifolia (Thunb.) Miers (TC), Phyllanthus niruri L. (PN), Andrographis paniculata (Burm.f.) Wall. ex Nees (AP), Lantana camara L. (LC), Mimosa pudica L. (MP), Justicia adhatoda L. (JA), Cuscuta reflexa Roxb. (CR), Cyperus rotundus L. (CyR), Piper nigrum L. (PiN), Ocimum sanctum Linn. (OS), Catharanthus roseus (L.) G.Don (CaR) and Clitoria ternatea L. (CT) are used to treat different ailments like malaria, rheumatoid, cold and cough, piles, diarrhoea and tooth ache etc. The present work aimed to evaluate the total phenolic and flavonoid contents as well as in vitro antioxidant properties of methanolic extract of medicinal plants. The total phenolic and flavonoid contents were determined by FolinCiocalteu and, aluminium chloride method with standard gallic acid and rutin respectively. Antioxidant activity was determined ...
Antioxidant activities and phenolic contents of the aqueous extracts of some Indian medicinal plants
2009
Antioxidants protect the body against oxidative stress by neutralizing free radicals. Plants contain rich amount of polyphenols which are very potent natural antioxidants. The present study was designed to evaluate the relative contribution of different polyphenols such as total phenolics, flavonoids and flavonol contents and their antioxidants activities. For this purpose the total phenolics, flavonoids and flavonol contents of some medicinal plants were determined in the aqueous extracts of leaves of Trichosenthes dioica, fruits of Moringa olifera and Ficus bengalensis as well as seeds of Emblica officinalis. Total antioxidant activity of these extracts was monitored by Free Radical Absorbing Power (FRAP) assay. In this paper, those parts of the plants are used for the analysis of aforesaid parameters which are normally overlooked. The total phenolic content of T. dioica leaves was about two times more than that obtained from the fruits and seeds of M. olifera and E. officinalis, respectively. However, the aerial roots of F. bengalensis registered presence of least phenolic content. The aqueous preparation from E. officinalis exhibited total flavonoid content twice as high as that of the other three plants. The extract from seeds of E. officinalis was found to contain highest antioxidant activity as compared to the preparations from other plants. The high antioxidant activity and flavonoids contents in E. officinalis seeds indicated that it could be exploited as an ingredient in developing a potential antioxidant supplement.
Screening of ten indian medicinal plant extracts for antioxidant activity
Currently there has been an increased interest globally to identify antioxidant compound that are pharmacologically potent and have low or no side effects. As plants are source of natural antioxidants, much attention has been gain to plants. The quest for natural antioxidants for dietary, cosmetic and pharmaceutical uses has become a major industrial and scientific research challenges over the last two decades. A variety of free radical scavenging antioxidants exist within the body in which many of them are derived from dietary sources like fruits, vegetables and teas. In this study methanolic crude extract of 10 traditionally used Indian medicinal plants were screened for their free radical scavenging and superoxide anion scavenging properties using Rutin and Curcumin as standard antioxidants. The overall antioxidant activity of ocimum sanctum was the strongest, followed in descending order by pterospermum acerifolium, Achyranthes aspera, Delonix regia, Mentha spicata, Datura stramonium, Coccinia indica, Hygrophilla auriculata, Cassia auriculata and Coriandrum sativum. Five plant, namely ocimum sanctum, pterospermum acerifolium, Achyranthes aspera, Delonix regia and Mentha spicata Showed strong super oxide anion scavenging activity. Both activities expressed as IC 50 and strong correlation showed between total polyphenolic contents in plants and their antioxidant activity.
Plant Science today, 2020
Natural antioxidant capacity of five important folk medicinal plants measured in vitro. Total phenolic content (TPC), flavonoid content (FC) and free radical scavenging capacity of ethanolic, methanolic and aqueous leaf extracts of Lippia alba (LA), Annona squamosa (AS), Hyptis suaveolens (HS), Commiphora wightii (CW) and Milletia pinnata (MP) was assessed using spectrophotometric method. Folin ciocalteu and aluminium chloride method employed to optimise TPC and FC. Free radical scavenging potentiality of leaf extracts was assessed using Ferrous ion chelation (FIC), 2, 2'-diphenyl-1-picrylhydrazyl (DPPH•) scavenging, Hydroxyl (OH•) radical scavenging (HRS) and Superoxide (O- 2) radical scavenging (SRS) methods. Results revealed that the TPC (96.22±5.85 to 519.23±34.90 ?g GAE/gm dry weight) were found significant in aqueous extracts from all the plants except AS (p<0.05). For FC (?g QCE/gm dry weight), ethanol was found optimum for LA (463.94±6.49), CW (289.99±2.70) and MP (34...
In the present study, we evaluated antioxidant activity and total flavonoid content of selected medicinally important plant species of India. Extracts of fruits of Rauwolfia tetraphylla, seeds of Nyctanthus arbortristis and leaves of Calotropis gigantea were prepared for the study. In-vitro models like DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging activity was carried out to investigate the antioxidant activity of methanolic plant extracts. Total flavonoid content of the plants was estimated using quercetin as standard to find out their contribution
Systematic evaluation of natural phenolic antioxidants from 133 Indian medicinal plants
Food Chemistry, 2007
Total antioxidant capacities of 133 Indian medicinal plant species sampled from 64 families were assessed by ABTS, DPPH and FRAP assays, and their total phenolic contents measured by Folin–Ciocalteu assay. These species exhibited a broad range of antioxidant activities, varying from 0.16 to 500.70 mmol TEAC/100 g DW in the ABTS assay. The antioxidant activity values similarly varied with the DPPH and FRAP assays. Significant and positive linear correlations were found between total antioxidant capacities and phenolic contents (R = 0.89–0.97), indicating that phenolics were the dominant antioxidant constituents in the tested medicinal plants. Preliminary identification of the major phenolic compounds from 83 selected medicinal plants by reversed-phase HPLC revealed phenolic acids, tannins, flavonoids, curcuminoids, coumarins, lignans, and quinines. The fruit of Terminalia chebula, pericarp of Punica granatum and gall of Rhus succedanea showed very high levels of hydrolysable tannins, and the gum of Acacia catechu presented very high levels of catechin and epicatechin in addition to tannins. Major phenolics in many of the medicinal plants were identified for the first time (e.g., Euphorbia lathyrus, Ipomoea turpethum, and Picrorrhiza kurroa). This systematic investigation of a large number of Indian medicinal plants proved important for understanding their chemical constituents and functionality in Ayurvedic medicine, and contributes to the search for natural sources of potent antioxidants.
2012
Indian medicinal plants (Casia fistula, Cinnamomum cassia, Acacia catechu and Citrus limon) were analyzed for their antioxidant activity, phytochemical composition and vitamins content. Methanolic extracts of the bark of these plant species showed high antioxidant activity and Casia fistula possessed the highest percent inhibition of DPPH (91.66%) amongst all studied plants. The results also revealed the presence of substantial amount of bioactive constituents comprising alkaloids (1.31 to 1.64 mg/100g DW), flavonoids (36.2 to 76.2 mg/100g DW), saponins (0.883 to 2.251 mg/100g DW), tannins (0.45 to 0.85 mg/100g DW) and total phenol content (110 to 210.2 mg/100g DW) where Casia fistula was observed comparatively richer source of these phytochemicals. The medicinal plants had the erratic concentrations of vitamins and contained carotenoids (104 to 135 mg/100g DW), ascorbic acid (18.05 to 55.04 mg/100g DW), thiamine (0.12 to 0.28 mg/100g DW), riboflavin (0.11 to 0.42 mg/100g DW) and ni...
PLANT SCIENCE TODAY, 2020
Natural antioxidant capacity of five important folk medicinal plants measured in vitro. Total phenolic content (TPC), flavonoid content (FC) and free radical scavenging capacity of ethanolic, methanolic and aqueous leaf extracts of Lippia alba (LA), Annona squamosa (AS), Hyptis suaveolens (HS), Commiphora wightii (CW) and Milletia pinnata (MP) was assessed using spectrophotometric method. Folin ciocalteu and aluminium chloride method employed to optimise TPC and FC. Free radical scavenging potentiality of leaf extracts was assessed using Ferrous ion chelation (FIC), 2, 2'-diphenyl-1-picrylhydrazyl (DPPH •) scavenging, Hydroxyl (OH •) radical scavenging (HRS) and Superoxide (O-2) radical scavenging (SRS) methods. Results revealed that the TPC (96.22±5.85 to 519.23±34.90 μg GAE/gm dry weight) were found significant in aqueous extracts from all the plants except AS (p<0.05). For FC (μg QCE/gm dry weight), ethanol was found optimum for LA (463.94±6.49), CW (289.99±2.70) and MP (347.47±4.50) whereas, aqueous was found more appropriate for rest two plants were found significant instead of ethanol and methanol (all p<0.05). The lowest IC50 (μg/ml) were recorded from A. squamosa (27.72±8.95), H. suaveolens (27.78±0.88), C. wightii (27.18±0.16) and M. pinnata (27.30±0.03). All plants have reflected a high antioxidant capacity; however, the highest antioxidant activity was reported from ethanolic extract of H. suaveolens followed by L. alba, A. squamosa, C. wightii and M. pinnata. Hence, these studies show that all folk medicinal plants contain potential antioxidant bioactive compounds.