Evaluation of Laboratory Methods to Measure Telomere Length in Cancer (original) (raw)

Telomere length measurement is crucial in understanding telomere biology, particularly in relation to cancer. Although Southern blot analysis has been the standard method, newer techniques such as quantitative PCR (qPCR) and quantitative fluorescence in situ hybridization (Q-FISH) offer improved accuracy and efficiency. This review evaluates various laboratory methods, such as qPCR, Q-FISH, and their modifications that enhance telomere length measurement. Despite advancements, issues with standardization remain, necessitating uniform protocols to ensure clinical applicability as a biomarker.