There is no relationship between Paraoxonase serum level activity in women with endometriosis and the stage of the disease: an observational study (original) (raw)
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Evaluation of oxidative stress in endometriosis: A case-control study
Caspian journal of internal medicine, 2015
Recent studies have suggested that oxidative stress (OS) may have a contribution in the pathogenesis of endometriosis. However, the results of previous studies regarding OS in endometriosis are controversial. The objective of this study was to compare the serum levels of OS markers in endometriosis versus the control group. This case-control study was carried out on 30 women with endometriosis aged 20-38 years presented to Fatemeh Zahra Infertility and Reproductive Health Research Center, Babol, Iran between March 2011 through November 2013. The serum samples of 40 women with same age were collected as the control group. The serum levels of malondialdehyde (MDA), carbonyl and iron were measured by photometric methods and compared between the patient and control groups using t-test. Also, we used ROC curve analysis to determine the discrimination ability of these markers. Serum iron in endometriosis patients was significantly higher than control (p<0.0001). Area under the ROC curv...
Measurable serum markers of oxidative stress response in women with endometriosis
Fertility and Sterility, 2009
Objective: To evaluate the hypothesis of increased systemic oxidative stress in patients with endometriosis. Setting: Tertiary care university hospital. Design: Cross-sectional study. Patient(s): Sixty-six women of reproductive age undergoing laparoscopy. Intervention(s): All women were investigated for endometriotic foci during laparoscopy. Forty-five women had laparoscopically and histologically confirmed endometriosis, and 21 women did not have endometriosis. Main Outcome Measure(s): Four markers of oxidative stress were assessed in the serum of each patient: heat shock protein 70 (HSP70), HSP70b 0 , thioredoxin (TRX), and ischemia-modified albumin (IMA). Result(s): Mean serum HSP 70b 0 level was higher in patients with endometriosis compared with controls (0.178 ng/mL, SD 0.103, and 0.135 ng/mL, SD 0.014, respectively). The disease stage did not affect HSP70b 0 levels. Heat shock protein 70, IMA, and TRX levels did not differ between patients with endometriosis and controls. Women with a history of arterial hypertension had higher mean IMA levels compared with women with normal blood pressure independently of the presence of endometriosis (106.7 [SD 25.4] U/mL and 85.0 [SD 11.5] U/mL, respectively). Conclusion(s): Endometriosis is associated with increased systemic oxidative stress. The implication of increased systemic oxidative stress in disease progression or the association with other oxidative stress-related pathologic conditions needs to be addressed in further studies.
Low-Density Lipoproteins Oxidation and Endometriosis
Mediators of Inflammation, 2013
The etiopathogenesis of endometriosis still remains unknown. Recent data provide new valuable information concerning the role of oxidative stress in the pathophysiology of the disease. It has been proved that levels of different lipid peroxidation end products are increased in both peritoneal fluid (PF) and serum of endometriotic patients. We assessed the concentration of oxidized low-density lipoproteins (oxLDL) in PF of 110 women with different stages of endometriosis and 119 women with serous ( = 78) or dermoid ( = 41) ovarian cysts, as the reference groups. PF oxLDL levels were evaluated by ELISA. We found that concentrations of oxLDL in PF of endometriotic women were significantly higher compared to women with serous but not dermoid ovarian cysts. Interestingly, by analyzing concentrations of oxLDL in women with different stages of the disease, it was noted that they are significantly higher only in the subgroup of patients with stage IV endometriosis as compared to women with ovarian serous cysts. In case of minimal, mild, and moderate disease, PF oxLDL levels were similar to those noted in reference groups. Our results indicate that disrupted oxidative status in the peritoneal cavity of women with endometriosis may play a role in the pathogenesis of advanced stages of the disease.
Assessment of serum paraoxonase and arylesterase activity in patients with endometrial cancer
European journal of gynaecological oncology, 2009
Serum paraoxonase (PON 1) is one of the most important enzymatic antioxidants that hydrolyzes lipid peroxidation, an indicator of carcinogenic activity. The aim of this study was to compare the serum levels of paraoxonase and arylesterase activity in patients with endometrial cancer to those of healthy controls. Serum paraoxonase and arylesterase activities, total free sulphydryl (-SH) groups and lipid hydroperoxide (LOOH) levels were measured in patients with endometrial cancer (n = 20) and controls (n = 23). Serum paraoxonase, arylesterase activities and total -SH group levels were significantly lower in patients compared to controls (p < 0.05, p < 0.05 and p < 0.001; respectively), while LOOH levels were significantly higher (p < 0.001). Among patients, serum paraoxonase and arylesterase activities were inversely correlated with LOOH levels (r = -0.680, p < 0.05; r = -0.708, p < 0.001; respectively), while these were positively correlated with the total -SH grou...
Oxidative stress biomarkers in patients with endometriosis: systematic review
Archives of Gynecology and Obstetrics, 2012
Purpose Since the first description about oxygen toxicity made by Joseph Priestley, the oxidative stress has been enrolled as a key factor in the pathogenesis of endometriosis. Our aim was to review oxidative stress biomarkers measured in patients with endometriosis. Methods Relevant studies were identified by searches of the MEDLINE database from 1990 to March 2011 using endometriosis, free radical and oxidative stress as mesh terms. We only included manuscripts in English, and review articles were excluded. In addition, free radical chemistry and oxidative stress history were discussed. Results After inclusion and exclusion criteria, 19 articles were selected to be included in this systematic review. A total of 36 oxidative stress biomarkers (20 different markers) were measured in patients with endometriosis. Some of the markers were measured in more than one manuscript. They were arranged in five subgroups: Enzymatic activity (n = 3), Anions/free radicals (n = 5), Lipoperoxidation markers (n = 7), DNA Damage markers (n = 1), and Protein oxidation (n = 4). Of those 36 markers, 23 were found to be significantly higher in patients with endometriosis comparing with control patients. Conclusion Oxidative stress plays an important role in the pathogenesis and progression of endometriosis. Keywords 8-Hydroxy-2-deoxyguanosine Á Cell toxicity Á DNA damage Á Endometriosis Á Free radical Á Oxidative stress
Pathophysiology, 2010
Oxidative stress is considered to be implicated in the pathophysiology of polycystic ovary syndrome (PCOS). This study was designed to evaluate the paraoxonase 1 (PON1) activity and oxidant/antioxidant status in Saudi women with PCOS and its contribution to the risk of atherosclerosis.Lipid profile, hormonal parameters, serum PON1 activity and oxidant (malondialdehyde)/antioxidant (total antioxidant capacity (TAC) levels were analyzed in 35 patients with PCOS and 30 healthy controls using a spectrophotometric method; correlation analysis was made between these variables. Insulin resistance was calculated by homeostasis model assessment (HOMA-IR).Women with PCOS had significantly higher fasting insulin, HOMA-IR and LH levels than controls. Lipid profiles and free androgen index (FAI) were significantly higher in women with PCOS when compared with controls. Serum PON1 activity was lower in the PCOS group (161.2 ± 6.1 U/l vs. 217.6 ± 9.3 U/l, p < 0.001) compared with controls, whereas malondialdehyde levels were higher in the PCOS group (4.26 ± 0.18 nmol/ml vs. 1.37 ± 0.12 nmol/ml, p < 0.001) compared with controls. Total antioxidant capacity was lower in the PCOS group (0.88 ± 0.10 mmol Trolox/l vs. 1.63 ± 0.17 mmol Trolox/l, p < 0.001) compared with controls. In PCOS group, serum PON1 was positively correlated with HDL-C (r = 0.425, p < 0.05) and TAC (r = 0.582, p < 0.01) but inversely correlated with HOMA-R (r = −0.54, p < 0.01), testosterone (r = −0.672, p < 0.01), FAI (r = −0.546, p < 0.01) and malondialdehyde (r = −0.610, p < 0.01).In conclusion, our data indicate that PON1 activity and antioxidant status were significantly decreased in Saudi women with PCOS. Lower serum PON1 activity might contribute to the increased susceptibility for the development of atherosclerosis risk in Saudi women with PCOS. Therefore, measurement of serum PON1 activity may be of value in assessment of women at higher risk for development of atherosclerosis risk in PCOS. However, further studies with larger sample size are needed to verify these results, and to assess the efficacy of antioxidant therapy on these patients.
Antioxidants
Can redox homeostasis indicators be potential non-invasive markers, crucial in the diagnosis and treatment of endometriosis? We checked if the differences in levels of serum oxidative-antioxidant balance parameters (TAS, FRAP, albumin, total bilirubin, uric acid, iron, SIRT3, SIRT5, SIRT6, telomerase, AOPP) are significant between patients with advanced endometriosis (E), healthy women (control group, C) and non-endometriosis women, but with other gynecological disorders (NE). The FRAP concentrations were significantly higher in E and NE group than in the control group (p = 0.015 and p = 0.017, respectively). The telomerase concentrations were significantly higher in the endometriosis group than in the control group (p = 0.004). Significantly higher concentrations of AOPP were observed in E (p < 0.001) and NE groups (p = 0.028) in comparison to the control subjects. Between stages III and IV of endometriosis, a significant difference existed only in concentration of iron (p = 0.0...
Association of Endometriosis and Oxidative Stress
European Journal of Medical and Health Sciences
Endometriosis is a gynecologic illness that affects women of reproductive age. The presence of endometrial tissue outside the uterine cavity distinguishes it. Pelvic discomfort and infertility plague the women who are affected. Retrograde menstruation, coelomic metaplasia, and induction theory are three main ideas that have been proposed to explain the complex etiology. Endometriosis development is also influenced by genetics and epigenetics. Recent research has focused on the role of oxidative stress, an imbalance between reactive oxygen species (ROS) and antioxidants, in the pathophysiology of endometriosis, which results in a peritoneal cavity inflammatory response. Reactive oxygen species (ROS) are inflammatory mediators that control cell growth and have harmful effects. They are formed by normal oxygen metabolism. A systematic review was conducted to understand better the many roles of oxidative stress and its role in the development of endometriosis. Iron metabolism, oxidative...