Screening inland halophytes from the central Balkan for their antioxidant activity in relation to total phenolic compounds and flavonoids: Are there any prospective medicinal plants? (original) (raw)
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Coastal Halophytes: Potent Source of Bioactive Molecules from Saline Environment
Plants
This study represents a comparative analysis of secondary metabolites content, antioxidant, and antimicrobial activity of 24 halophytes from coastal saline habitats of the Balkan Peninsula (Montenegro, Albania, and Greece). Total content of phenolics, flavonoids, tannins, anthocyanins, antioxidant, and antimicrobial activity was determined for dry methanolic (DME) and crude water extracts (CWE) and compared with well-known medicinal plants. The total phenolic content ranged from 13.23 to 376.08 mg of GA/g of DME, and from 33.68 to 511.10 mg/mL of CWE. The content of flavonoids ranged from 12.63 to 77.36 mg of RU/g of DME, and from 12.13 to 26.35 mg/mL of CWE. Total tannins and anthocyanins varied from 0.05 to 2.44 mg/mL, and from 1.31 to 39.81 µg/L, respectively. The antioxidant activity ranged from 1147.68 to 15.02 µg/mL for DME and from 1613.05 to 21.96 µg/mL for CWE. The best antioxidant properties, and the highest content of phenolic compounds, were determined for Polygonum mari...
Journal of Pharmacognosy and Phytochemistry, 2013
Halophytic plants are more susceptible for oxidative stress and damage due to high contents of salt and minerals inside these plants. Therefore, self defence against this oxidative stress appeared in the high phenolics particularly, flavonoids content are abundant in these plants. Mesembryanthemum crystallinum, Limoniastrum guyonianum and Anabasis articulate are three of halophytic plants growing in Mediterranean coast of Libya and most of North African countries, were taken as example for estimating the phenolic and flavonoids contents as well as antioxidant evaluation in order to understanding the effect of habitat of these plant imitation on the by-products production. Our present work suggested that, there are high relations between the qualitative and quantitative constituent of these halophytic plants which growing near to each other in the same environment.
The aim of this study was to investigate some biochemical attributes with an adaptive role for the soil salinity for nine halophyte species known as medicinal plants. Halophytes developed different mechanisms for adapting to abiotic stress action, by increased antioxidant activity. Thus, the content of total polyphenols and flavonoids was investigated in several halophyte species collected in vegetative and flowering phenophases in summer of 2012 from saline habitats located in Sulina, Murighiol and Histria (Tulcea county).
Comptes Rendus Biologies, 2008
Halophyte ability to withstand salt-triggered oxidative stress is governed by multiple biochemical mechanisms that facilitate retention and/or acquisition of water, protect chloroplast functioning, and maintain ion homeostasis. Most essential traits include the synthesis of osmolytes, specific proteins, and antioxidant molecules. This might explain the utilization of some halophytes as traditional medicinal and dietary plants. The present study aimed at assessing the phenolic content and antioxidant activities of some Tunisian halophytes (Cakile maritima, Limoniastrum monopetalum, Mesembryanthemum crystallinum, M. edule, Salsola kali, and Tamarix gallica), depending on biological (species, organ and developmental stage), environmental, and technical (extraction solvent) factors. The total polyphenol contents and antioxidant activities (DPPH and superoxide radicals scavenging activities, and iron chelating and reducing powers) were strongly affected by the above-cited factors. Such variability might be of great importance in terms of valorising these halophytes as a source of naturally secondary metabolites, and the methods for phenolic and antioxidant production. To cite this article: R. Ksouri et al., C. R. Biologies 331 (2008). Résumé Influence des facteurs biologiques, environnementaux et techniques sur les teneurs en polyphénols et les activités antioxydantes des halophytes tunisiennes. La capacité des halophytes à surmonter le stress oxydatif déclenché par la salinité est régi par de multiples mécanismes biochimiques qui facilitent le maintien et/ou l'acquisition de l'eau, la protection des chloroplastes et le maintien de l'homéostasie ionique. Ces traits comprennent essentiellement la biosynthèse d'osmolytes, de protéines spécifiques et de molécules antioxydantes. D'où, l'utilisation traditionnelle de ces halophytes comme plantes à intérêts médicinales et alimentaires. On se propose, dans ce travail d'évaluer les teneurs en polyphénols et les activités antioxydantes de quelques halophytes tunisiennes (Cakile maritima, Limoniastrum monopetalum, Mesembryanthemum crystallinum, M. edule, Salsola kali et Tamarix gallica) en fonction des facteurs biologiques, environnementaux et techniques. L'analyse des résultats a montré que les teneurs en polyphénols, les activités antiradicalaires et les pouvoirs chélateur et réducteur sont significativement affectés par ces différents facteurs. Une telle variabilité pourrait être d'une grande importance dans la valorisation de ces halophytes comme source naturelle de biosynthèse d'antioxydants. Pour citer cet article : R. Ksouri et al., C. R. Biologies 331 (2008).
ANTIOXIDANT PROPERTIES AND PHENOLIC COMPOSITION OF COASTAL HALOPHYTES COMMONLY USED AS MEDICINE
Halophytes are well adapted in extreme environmental conditions, regulation of secondary metabolism is one of the keys of their success. In this study five coastal halophytes i.e. Atriplex stocksii, Cressa cretica, Heliotropium bacciferum, Ipomea pes-caprae and Salsola imbricate, which are well known for their therapeutic properties were investigated for their antioxidant activity and polyphenol composition. Medicinal halophytes showed considerable antioxidant activity in terms of DPPH (14.4-64.8 I%), ABTS (22.6-230.6 µMol TE g-1), FRAP (0.5-5.6 mMol Fe +2 g-1) and TAC (17.6-36.1 mg AsA g-1). Results indicated that these plants also contained high amount of total phenols (7.7-29.6 mg GAE g-1), flavonoids (4.2-17.3 mg QE g-1) and proanthocynadins (0.2-0.6 mg CE g-1). Among these, I. pes-caprae and C. cretica had the highest antioxidant activity and polyphenolic contents. High correlation among antioxidant activity assays (r = 0.877-0.999) indicated the radical scavenging and reducing power abilities of these plants. Similarly, strong correlations (r = 0.654-0.953) among antioxidant activity measurements and polyphenolic composition suggested that phenolic compounds contributed mainly to the antioxidant activity of these plants. Present study reveals coastal halophytes as rich sources of natural antioxidants, which could be used in herbal formulations, pharmaceuticals/ nutraceuticals, food additives and cure for ailments related to oxidative stress. Furthermore, these plants could be grown using saline resources and provide bioactive raw material with high industrial and economic value.
Aob Plants, 2014
Halophytes, salt-tolerant plants, are a source of valuable secondary metabolites with potential economic value. The steady-state pools of many stress-related metabolites are already enhanced in halophytes when compared with glycophytes, but growth under conditions away from the optimum can induce stress and consequently result in changes to secondary metabolites such as antioxidants. However, direct evidence for increasing the concentration of valuable secondary metabolites as a consequence of altering the salinity of the growing environment still remains equivocal. To address this, we analysed a range of metabolites with antioxidant capacity (including total phenols, flavonoids, ascorbate, reduced/oxidized glutathione and reactive oxygen species scavenging enzymes) in seedlings and plants from different families (Amaranthaceae, Brassicaceae, Plantaginaceae and Rhizophoraceae) and habitats grown under different salt concentrations. We show that it is possible to manipulate the antioxidant capacity of plants and seedlings by altering the saline growing environment, the length of time under saline cultivation and the developmental stage. Among the species studied, the halophytes Tripolium pannonicum, Plantago coronopus, Lepidium latifolium and Salicornia europaea demonstrated the most potential as functional foods or nutraceuticals.
Journal of Agricultural Science and Technology, 2019
In the last decades, an increasing interest has been granted to halophytes due to their high phenolic content, which have therapeutic potential in the treatment and/or management of human health. Therefore, it is important to measure the halophyte total polyphenol content correctly and to valorize their antioxidant capacity. Ethanol extracts from thirty halophytes were analyzed to evaluate the Total Phenol Content (TPC). We employed three testing methods to prove their antioxidant potentialities, including DPPH • (1-DiPhenyl-2-PicrylHydrazyl), ABTS •+ (2,20-Azino-Bis-3-ethylbenzoThiazoline-6-Sulfonic acid) and IRP (Iron Reducing Power) assays. Results showed that plants exhibited different TPC, which varied significantly from 411.5 mg GAE g-1 DW in Cynomorium coccineum to 6.02 mg GAE g-1 DW in Ammophila arenaria. Concerning antioxidant activities, data revealed that Cynomorium coccineum (IC 50 = 3.82 µg ml-1 versus ABTS •+) and Euphorbia paralias had the highest antiradical capacity (IC 50 = 0.12 µg ml-1 against DPPH •) and exhibited the best efficient concentration with an EC 50 value= 9.57 µg mL-1 for the IRP. Considering correlation between phenols and antioxidant tests, three groups were distinguished with a higher correlation coefficient between 0.78 and 0.98 for the first group. These data suggest the promising potentialities of the Mediterranean medicinal halophytes as valuable source of powerful antioxidants of industries, especially for Cynomorium coccineum, Carpobrotus edulis, Reaumuria vermiculata, Tamarix gallica, and Euphorbia paralias regarding their strong phenol content.
Molecules
Halophytes have been characterized as a potential resource for fiber, food, fodder, and bioactive compounds. Proximate composition, bioactive compounds, and antioxidant activity of five wild dominant halophytes (Arthrocnemummacrostachyum, Halocnemumstrobilaceum, Limoniastrummonopetalum, Limoniastrumpruinosum, and Tamarix nilotica) naturally growing along the Nile Delta coast were assessed. The soil supporting these halophytes was sandy to sand-silty, alkaline, with low organic carbon, and relatively high CaCO3. H. strobilaceum attained the highest moisture content, ash, crude fiber, lipids, and total soluble sugars. L. monopetalum showed the highest content of crude protein (18.00%), while T. nilotica had the highest content of total carbohydrates. The studied halophytes can be ranked according to their nutritive value as follows: H.strobilaceum > L.monopetalum > A.macrostachyum > L.pruinosum > T. nilotica. A. macrostachyum attained the highest amount of Na+, K+, Ca2+, a...
Plant Physiology and Biochemistry, 2007
Cakile maritima is a local oilseed halophyte exhibiting potential for secondary metabolite production. In the present study, plant growth, leaf polyphenol content and antioxidant activity were comparatively analyzed in two C. maritima Tunisian accessions (Jerba and Tabarka, respectively sampled from arid and humid bioclimatic stages) under salt constraint. Three-week-old plants were subjected to 0, 100, and 400 mM NaCl for 28 days under glasshouse conditions. A significant variability in salt response was found between both accessions: while Tabarka growth (shoot biomass, leaf expansion) was significantly restricted at 100 and 400 mM NaCl, compared to the control, Jerba growth increased at 100 mM before declining at 400 mM NaCl. The better behaviour of Jerba salt-challenged plants, compared to those of Tabarka, may be related to their higher polyphenol content (1.56- and 1.3-fold the control, at 100 and 400 mM NaCl respectively) and antioxidant activity (smaller IC50 values for both 1,1-diphenyl-2-picrylhydrazyl and superoxide scavenging), associated with lower leaf MDA accumulation (ca. −66% of the control at 100 mM NaCl). Taken together, our findings suggest that halophytes may be interesting for production of antioxidant compounds, and that the accession-dependent capacity to induce antioxidative mechanisms in response to salt, may result in a corresponding variability for growth sustainability.