Status of oxidant, antioxidantand serum enzymes in thalassaemic children receiving multiple blood transfusions (original) (raw)
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Antioxidant and Hematological Study among Egyptian Thalassemic Children
2013
Patients with beta thalassemia need repeated blood transfusion for survival which may cause oxidative stres s and tissue injury due to iron overload and altered antioxidant enzymes. Our review aiming to study the anti oxidant levels changes in Egyptian thalassemic children and recognize either the antioxidant changes is due to associated repeated blood transfusion or due to the disease itself. We also monitor the damage occur by a ntioxidant on liver functions.
Antioxidant status in children with homozygous thalassemia
2005
The status of enzymatic and non-enzymatic anti-oxidants was evaluated in 41patients with transfusion dependent β−thalassemia. An additional 20 age-matched children, with non-hemolytic anemia, served as controls. Fresh blood samples, obtained in the morning, were processed immediately. Plasma was stored at -80ºC. Levels of vitamins A and E were assayed simultaneously by HPLC. RBC vitamin A was not measurable in 29 (70. 7%) thalassemics and in all the controls. Plasma vitamin A levels were lower in thalassemics than in controls (p<0. 05). Vitamin E in RBCs was not measurable in 13 (31. 7%) cases. The mean level of RBC vitamin E was 3 times lower in thalassemics. Similarly, SOD enzyme activity in thalassemics, was at least 1.5 lower in comparison to the activity documented in controls (p<0. 05). The observations indicate that thalassemics have enhanced oxidative stress. Administration of selective antioxidants and a balanced diet may preclude oxidative damage.
The Eurasia Proceedings of Science Technology Engineering and Mathematics, 2021
Repeated blood transfusion in beta thalassemia patients may lead to peroxidative tissue injury by secondary iron overload. In the present study, (43) patients with beta thalassemia. We have evaluated hemoglobin (Hb), packed cell volume (PCV), red blood cells (RBC), white blood cells (WBC), iron (Fe), ferriten, uric acid, glutathion (GSH), malondialdehyde (MDA), Vitamins C and E and electrolytes as sodium (Na), potassium (K), and chloride (Cl). The findings were compared with (25) age matched healthy individuals were included in this study as a control group. A significant increase in the levels of WBC, Fe, ferritin, MDA and Vit C (P < 0.001), whereas significant decrease in the levels of Hb, PCV, RBC, GSH and Vit E (P < 0.001) was observed. Uric acid, Na and K were significant increase (P < 0.05) in the patients when compared with controls, while there was a non-significant increase in mean value of Cl. These results were suggesting that oxidative stress and reduced antioxi...
Oxidative stress in pediatric patients with β thalassemia major
Background β-thalassemia major (β-TM) is a common inherited hemolytic type of anemia. Repeated blood transfusions predispose β-TM patients toward peroxidative tissue injury because of secondary iron overload. Objectives This study aimed to evaluate the effects of iron overload on antioxidant enzymes and liver cell damage in β-TM patients undergoing regular blood transfusions. Patients and methods This prospective case–control cohort study included 30 pediatric patients with a confirmed diagnosis of β-TM on regular blood transfusions and 20 agematched and sex-matched healthy children attending the Qena University Hospital, Pediatric Clinic. Blood samples were withdrawn from each patient to measure serum levels of ferritin, glutathione peroxidase (GPX), and superoxide dismutase (SOD). Results Total bilirubin, aspartate transaminase (AST), alanine transaminase (ALT), and ferritin levels were significantly higher in the β-TM group (P<0.001, 0.001, 0.001, and 0.001, respectively), whereas GPX and SOD were significantly lower in the β-TM group (P<0.001 and 0.001). The correlation between serum ferritin level and age, bilirubin, AST, and ALT in patients group showed that, the correlation between serum ferritin level and age was (0.745) while Pvalue was<0.001, the correlation between serum ferritin level and bilirubin level was (0.665) while P-value was <0.001, the correlation between serum ferritin level and (AST) level was (0.727) while P-value was <0.001 and the correlation between serum ferritin level and (ALT) level was (0.737) while P-value was <0.001. The correlation between SOD and age, ferritin, bilirubin, AST, and ALT in patients group in patients group showed that, the correlation between (SOD) and age was (−0.454) while P-value was 0.012, the correlation between (SOD) and ferritin level was (−0.664) while P-value was<0.001, the correlation between (SOD) and bilirubin level was (−0.535) while P-value was 0.002, the correlation between (SOD) and (AST) level was (−0.567) while P-value was <0.001 and the correlation between (SOD) and (ALT) level was (−0.558) while P-value was <0.001. Conclusion Impaired levels of antioxidant enzymes SOD and GPX in patients with β-TM on repeated transfusion, in addition to excessive free iron concentration, iron overload may attribute to oxidative damage in these patients. Antioxidant systems that compensate for reduced lipid peroxidation to lower tissue damage are needed. Egypt J Haematol 2017 42:123–127 © 2017 The Egyptian Journal of Haematology
Lipid Peroxidation and Antioxidant Status in β-Thalassemic Patients: Effect of Iron Overload
To study the effect of iron overload due to continuous blood transfusions on peroxidation products, such as malondialdehyde (MDA) and peroxynitrite, with evaluation of some antioxidants like, glutathione (GSH), superoxide dismutase (SOD), vitamin A, vitamin C, vitamine E, Ceruloplasmin, uric acid and albumin in thalassemia patients. Forty patients with thalassemia major, aged 5 to 15 years, were carried out in Abn-Alatheer Teaching Hospital in Mosul city, during the period from October 2007 to April 2008. They were on Chelation therapy with desferrioxamine. They were divided into two groups, the first one without iron overload (90,97±12.92), and the second one with iron overload (157.75±7.57). All the patients were received whole blood. Blood samples were collected before and after blood transfusion. The results showed that there were significant increase in MDA and peroxynitrite in patients with iron overload five days before and after blood transfusion in compared with groups having normal iron level. On the other hand, glutathione, superoxide dismutase activity, Vitamin A, vitamin C, vitamin E, albumin and ceruloplasmin were significantly decreased whereas, uric acid was increased significantly. It is concluded that, Iron over load due to continuous blood transfusion in thalassemia causes increase in oxidative tissue damage with a changes in antioxidants status.
Background: Sickle cell disease (SCD) often leads to chronic hemolytic anemia of varying severity, and blood transfusion may be employed in the management of SCD complications. Objective: The aim of the study was to evaluate the effect of blood transfusion on the activities of some antioxidant enzymes as well as lipid peroxide and to relate transfusion status to these enzymes and malondialdehyde (MDA) in SCD patients. Materials and Methods: Glutathione peroxidase (GPX), superoxide dismutase, catalase, MDA, and lipoproteins were assayed in 87 SCD and 20 age-and sex-matched subjects with normal hemoglobin. Of the 87 SCD patients, 30 had multiple transfusions, 21 had been transfused once while 36 had not been transfused within the last 3 months. Results: Statistically significant decrease in the mean levels of GPX (P = 0.045) and Cu/Zn SOD (P = 0.001) and increased (P = 0.001) MDA were observed in the transfused compared to non-transfused patients. Similarly, significant decrease (P = 0.001) in Cu/Zn SOD and increase (P = 0.01) in MDA were observed in multi transfused compared to those who had been transfused once. Transfusion status correlated (P <0.047) inversely with Cu/Zn SOD and positively with MDA. Conclusion: Reduced activity levels of serum antioxidant enzymes and increased mean levels of MDA were observed in transfused compared to non-transfused SCD patients and these changes correlated with transfusion status.