CIL:26530, Mus musculus, fibroblast. In Cell Image Library (original) (raw)

2021

Abstract

To study the molecular mechanism by which nonmuscle myosin II (MII) regulates protrusion and adhesion dynamics in migrating cells, NIH3T3 cells were transfected with myc-tagged phosphomimetic constitutively active MLC mutants (MLCaa, green). After 20' exposure to PDGF, which induces myosin-inactivation, stress fiber disassembly, and stimulates fibroblast motility, the cells were fixed and co-stained for TRIO (a multifunctional, multidomain Rho guanine nucleotide exchange factor, red) and filamentous actin (blue). These findings help elucidate a functional link between MII and Rac1/Cdc42 GTPases, which may regulate protrusion/adhesion dynamics in migrating cells. This image is original data from Fig. S5 "Expression of active MLC mutants inhibits the dissociation of MII–Trio complex," in J. Cell Biol. 2010. Vol. 190(4):663–674

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