Abstract A091: Specific myelomonocytic cells heavily infiltrate orthotopic lung tumors and display a hypoxia-driven miRNA expression signature that directs tumor-supporting functions and negatively impacts on clinical outcome (original) (raw)

Cancer Immunology Research, 2016

Abstract

Tumor-derived micro-RNA expression patterns are known to convey prognostic information in lung cancer. However, the cellular source of these signatures is unclear. In parallel, immunological parameters such as the presence and activation status of dendritic cells (DCs) emerge as important prognostic factors in this disease as well, presumably by affecting the quality of immune surveillance.We hypothesized that tumor-associated DCs are involved in the way tumor-derived miRNA expression patterns impact on disease outcome.Using a preclinical model of lung cancer featuring orthotopic tumor growth in syngeneic, immunocompetent hosts, we found that lung tumors were preferentially infiltrated by CD11c+/MHCII+/CD11b+/CD103- DCs (TIDCs). Compared to their peritumoral counterparts, displayed a dramatic overexpression of PD-L1 along with markers associated with tumor-associated macrophages (TAM). Transcriptome analysis of these CD11b+ TIDCs vs peritumoral CD11b+ DCs using GSEA and ISMARA indic...

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