Nondiphtherial Corynebacterium species isolated from clinical specimens of patients in a university hospital, Rio de Janeiro, Brazil (original) (raw)
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Antibiotics
Antimicrobial resistance is one of the most important public health issues. Besides classical multidrug resistance species associated with medical care involved in superficial or invasive infections, there are strains less commonly associated with hospital or outpatient setting’s infections. Non-diphtheria Corynebacterium spp. could produce infections in patients with or without immune-compromised status. The aim of our study was to determine the susceptibility to antimicrobial agents to Corynebacterium spp. from clinical samples collected from Romanian hospitalized individuals and outpatients. Twenty Corynebacterium strains were isolated and identified as Corynebacterium striatum (n = 7), Corynebacterium amycolatum (n = 7), C. urealyticum (n = 3), Corynebacterium afermentans (n = 2), and Corynebacterium pseudodiphtheriticum (n = 1). All isolates have been tested for antibiotic susceptibility by standardized disc diffusion method and minimal inhibitory concentration (MIC) tests. Sev...
Antimicrobial Susceptibility Testing for Corynebacterium pseudodiphtheriticum
https://ijshr.com/IJSHR\_Vol.5\_Issue.4\_Oct2020/IJSHR\_Abstract.0023.html, 2020
Background: Corynebacterium pseudodiphtheriticum is harmless to humans and only part of commensal in the upper respiratory tracts and skin. Nevertheless Over the last two decades C.pseudodiphtheriticum has been reported was caused disease in humans, particularly in patients with impaired immunity. Currently information about C.pseudodiphtheriticum resistance in Indonesia is still limited. Objectives: This study aims to determine patterns of resistance and the effectiveness of some antibiotics against C.pseudodiphtheriticum. Method: The determinations of antibiotic resistance pattern are performed by using diffusion disc agar methode. using the medium Muhler hilton with the addition of 5% sheep blood. The results of antibiotic susceptibility testing for C.pseudodiphtheriticum showed all isolates resistance to erythromycin and tetrasikline. Results: There are two isolates showed resistance against to penicillin. In contrary all isolates are sensitive to ciprofloxacin and ceftriaxone. Resistance profile also showed multi drug resistance against to 2, 3, 4 and 7 antibiotics such as penicillin, moxifloxacin, gentamicin, vancomycin, tetracyclines, clindamycin and linezolid. Conclusion: There are monoresistant C. pseudodiphtheriticum and multi-drug resistant to some antibiotics.
Hardware Infection with Corynebacterium spp.: a Case Report and Review of the Literature
Clinical Microbiology Newsletter, 2014
A 58-year-old man with a history of psoriatic arthritis presented with a post-operative spine infection 4 months following an L3-4 spinal fusion and laminectomy. His past medical history included coronary artery disease, hypertension, dyslipidemia, type II diabetes, squamous cell skin cancer, and an initial post-operative course complicated by a surgical site infection due to group B streptococcus approximately 1 month after initial hardware placement, which was managed by irrigation and debridement (I&D), 9 days of parenteral therapy (predominantly cefazolin), and 8 weeks of cephalexin. Several subsequent I&Ds produced no organisms recovered by culture. At the time of presentation, he had a large amount of drainage from the distal aspect of his surgical wound, which again required I&D. The patient's intraoperative cultures included three swabs and one tissue sample taken directly from the surgical site. White blood cells were present in low density in 3 of 4 smears, but no organisms were seen in any of the four direct Gram stain smears. Only the lumbar tissue sample was positive for growth. Growth on sheep blood agar at 35°C in CO 2 revealed an essentially pure culture of 0.5mm pinpoint, grey-white, round, non-hemolytic colonies, and the Gram stain demonstrated palisading coryneform gram-positive bacilli. No fungi, anaerobes, or acid-fast bacteria (AFB) were recovered by culture.. The organism was phenotypically identified as Corynebacterium jeikeium by the API ® Coryne test kit (identification number 2100304; bioMériuex, Marcy l'Etoile, France). However, 16S rRNA gene sequencing revealed that the sequence was not identical to that of C. jeikeium but was found in a closely related genogroup (Fig. 1) that differed by ten base pair deletions (Fig. 2). The organism was resistant to penicillin, erythromycin, and clindamycin (by disk diffusion using Staphylococcus breakpoints according to CLSI M100 (1). It was susceptible to vancomycin (MIC, 1.0 μg/ml), linezolid (MIC, 0.38 μg/ml), doxycycline (MIC, 0.38 μg/ml), and rifampin (MIC, 1.0 μg/ml) but resistant to ciprofloxacin (MIC, 12 μg/ml) by
Aerobic, Gram positive, catalase positive and non-spore forming bacilli, which are morphologically similar to Corynebacterium diphtheriae are described as either diphtheroids or coryneform bacteria, resembling C diphtheriae. Corynebacteria are a group of bacteria placed under the family corynebacteriaceae, which come under the phylum, Actinobacteria. Among the members of genus Corynebacterium, only C diphtheriae is considered as a pathogen but other species are present either as normal flora in human or as saprophytes in the environment and have rarely been associated with human infections. Of late, there have been increased reports of both new species of genus Corynebacterium and their occurrence in various human infections. It is now imperative that clinical microbiologists and clinicians understand the potential role of NDC in human infections. Only few studies globally have characterized the human clinical isolates of NDC and their antimicrobial susceptibility patterns. This review tries toexamine? the potential pathogenic nature of NDC, which warrants their identification and prompt reporting when isolated from human clinical specimens.
Scientific reports, 2017
Corynebacterium striatum is a nosocomial opportunistic pathogen increasingly associated with a wide range of human infections and is often resistant to several antibiotics. We investigated the susceptibility of 63 C. striatum isolated at the Farhat-Hached hospital, Sousse (Tunisia), during the period 2011-2014, to a panel of 16 compounds belonging to the main clinically relevant classes of antimicrobial agents. All strains were susceptible to vancomycin, linezolid, and daptomycin. Amikacin and gentamicin also showed good activity (MICs90 = 1 and 2 mg/L, respectively). High rates of resistance to penicillin (82.5%), clindamycin (79.4%), cefotaxime (60.3%), erythromycin (47.6%), ciprofloxacin (36.5%), moxifloxacin (34.9%), and rifampicin (25.4%) were observed. Fifty-nine (93.7%) out of the 63 isolates showed resistance to at least one compound and 31 (49.2%) were multidrug-resistant. Twenty-nine resistance profiles were distinguished among the 59 resistant C. striatum. Most of the str...
Journal of clinical microbiology, 1996
A comprehensive study was performed on 25 bacterial clinical isolates originally identified as Corynebacterium xerosis. Three reference strains of C. xerosis were also included in the study. On the basis of a variety of phenotypic characteristics tested, all strains could be divided into two separate clusters: reference strains ATCC 373 (the type strain of C. xerosis) and ATCC 7711 showed yellow-pigmented, dry, rough colonies, fermented 5-keto-gluconate, exhibited strong leucine arylamidase and alpha-glucosidase activities, produced lactate as the major end product of glucose metabolism, were susceptible to most of the 19 antimicrobial agents tested, and showed an inhibition zone around disks containing the vibriocidal compound O/129. In contrast, the remaining 26 strains including reference strain NCTC 7243 as well as all clinical isolates formed white-grayish, dry, slightly rough colonies, did not ferment 5-keto-gluconate, exhibited only weak leucine arylamidase and no alpha-gluco...
Corynebacterium macginleyi` a rare bacteria causing infection in an immunocompromised patient
Journal of Cancer Research and Therapeutics, 2010
Corynebacterium species are normal flora of skin and mucous membrane. In recent years, coryneforms have emerged as important opportunistic pathogens in immunocompromised patients. Majority of the Corynebacterium macginleyi isolates are from conjunctiva and cornea. The only reported non ocular isolates are from urinary tract infection, intra-venous catheter related infection, valvular endocarditis and septicaemia. We report herein a rare case of C. macginleyi isolated from tracheostomy site secretions in a patient with carcinoma larynx which was treated successfully with Cefoperazone-sulbactum for seven days and replacing tracheostomy tube. This is the first case of C. macginleyi reported from India.
Journal of Infection and Public Health, 2013
A prospective study was conducted to assess the role of coryneform bacteria in surgical site infections among obstetric and gynecological patients undergoing surgery. The surgery was graded according to the degree of contamination, and surgical site infections (SSIs) were classified as superficial or deep. Pus samples were collected from SSIs according to rigorous aseptic precautions, and the quality of specimens was assessed by Q-score. A detailed clinical and treatment history was elicited from all patients. The samples were processed using standard protocols. Coryneform bacteria were considered significant pathogens only if they fulfilled rigorous clinical and microbiological criteria. Antibiotic susceptibility testing was performed using the Kirby-Bauer method according to the CLSI guidelines. In total, 127 patients developed SSIs among 882 postoperative patients. Of these, 89 (70.1%) were culture positive: 40 (44.9%) were Gram-positive cocci, 27 (30.3%) were coryneform, and 22 (24.7%) were Gram-negative bacilli. All coryneform-infected patients had fever and post-operative wound dehiscence leading to a prolonged hospital stay. The most commonly isolated organism was Staphylococcus aureus (33.7%), followed by Corynebacterium amycolatum (11.2%), Escherichia coli (8.9%), Citrobacter spp. (7.8%) and coagulase-negative Staphylococci (6.7%). In our study, 45.5% were ESBL producers, 18.2% were Amp C producers, and 40% were MRSA. All the coryneform bacteria were multidrug resistant, and 51.8% of isolates were sensitive to only gatifloxacin and vancomycin. Symptomatic improvement was observed in all coryneform-infected patients after the administration of appropriate therapy. Coryneform bacteria appear to be emerging as significant nosocomial surgical site pathogens. The high level of multidrug resistance observed in coryneform bacteria in our study is cause for alarm.
Antimicrobial Agents and Chemotherapy, 2018
Non- diphtheriae Corynebacterium -associated disease has been increasingly observed and often presents a conundrum to the treating physician. Analysis of antibiotic susceptibility testing data for 1,970 clinical Corynebacterium isolates received between 2011 and 2016 revealed that empirical drug treatment options are limited to vancomycin and linezolid. Corynebacterium striatum was the most frequently observed species during this study period, along with C. amycolatum and C. pseudodiphtheriticum/C. propinquum . Low levels of susceptibility to penicillin (14.5%), erythromycin (15.1%), and clindamycin (8.7%) were observed for non- diphtheriae Corynebacterium species, while 3.0% of isolates were not susceptible to daptomycin. Similarly, 26.9% and 38.1% of Corynebacterium isolates were susceptible to ciprofloxacin and trimethoprim-sulfamethoxazole, respectively. Our data show much lower susceptibility to penicillin than previously reported in the literature and an increasing number of i...
2017
The infections produced by non-diphtheriae Corynebacterium spp. in Romanian patients are poorly documented and probably neglected. The aim of our study is to characterize Corynebacterium strains collected from Romanian hospitalized individuals and outpatients in order to be identified by phenotypic and molecular methods.Twenty Corynebacterium spp. isolates were identified at the species level using phenotypic and molecular techniques. Phenotypic identification consisted in standard microbiological methods: Gram stain, catalase and oxidase tests and biochemical characterization using the commercial API Coryne kit according to the manufacturer's instructions and Maldi-toff method. The final identification of species was achieved by molecular technique, namely ribosomal DNA sequence analysis. The strains recovered from human clinical samples (six from blood culture, five isolates from wounds, two from peritoneal fluid, three from urine, one from a drainage catheter, one from perire...