Microbiological profile of diabetic foot ulcers and its antibiotic susceptibility pattern in a teaching hospital, Gujarat (original) (raw)
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Bacterial Profile of Diabetic Foot Ulcer - Study From Western India
International Journal of Health Sciences and Research, 2016
Introduction: Diabetic foot ulceration and infections are a major medical, social, economic problem and a leading cause of morbidity and mortality, especially in the developing countries like India. Fifteen percent of all diabetics develop a foot ulcer at some point in their lives. Aim and objectives: To Study the bacterial profile of diabetic foot ulcer. To isolate and identify aerobic bacteria from diabetic foot ulcers and to determine their antibiotic resistance pattern Material and methods: This was a prospective study, conducted over a period of 1 year. 75 diabetic patients with foot ulcer attending the Surgery OPD were included in the study. Two swabs were taken from each patient into Amie’s transport medium and processed by standard techniques to isolate and identify the bacteria. Antibiotic susceptibility testing was done by Kirby-Bauer’s disc diffusion method for all the isolated bacteria. Results: A total of 110 aerobic organisms were isolated from 75 cases. Organisms isol...
International Journal of Research in Medical Sciences, 2014
Diabetes mellitus is a worldwide disorder and affects many people. Type 2 diabetes is the most common form of diabetes in developing countries like India, Africa and China. At present 31.7 million people are diabetic in India. Hence, it has been labelled as "The diabetic capital of the world". 1 Diabetes is considered to be a serious disorder not just for its predominance but for its complications. One such important complication is the ABSTRACT Background: Diabetic foot infections are important cause of morbidity and mortality among persons with diabetes mellitus. The reported prevalence rates in India range from 0.9-8.3%. Diabetes foot lesions are the leading cause of non-traumatic amputations worldwide. A study has been conducted to isolate and find the antibiotic susceptibility pattern of the bacteria from diabetic foot infections from the patients of Kancheepuram district, Tamil Nadu, India. Methods: Sixty patients previously diagnosed or newly diagnosed as diabetic, presented with lower extremity infection attending Tagore medical college and hospital and its peripheral centres were selected for the study. Various specimens (pus, wound exudates, or tissues biopsy) for microbiological studies were obtained from the infected region. The specimens were cultured on blood agar and MacConkey agar for aerobic / facultative anaerobic organisms and on Neomycin Blood Agar for anaerobic organism. The plates were then incubated at 37°C. For anaerobic culture the plates were incubated in the McIntosh anaerobic jar. Isolates obtained are identified by standard laboratory techniques. Results: The result showed that Pseudomonas aeruginosa (48.3%) is the predominant bacterium followed by Staphylococcus aureus (38%) and other bacteria. The anaerobic bacteria are also isolated from the diabetic foot ulcers. The Peptostreptococcus species (26.7%) are the predominant bacteria followed by other bacteria. Further the results showed that 22 patients (37%) showed the multi-bacterial infection and remaining 38 patients (63%) showed mono bacterial infection. The drugs like amikacin, cefepine, ciprofloxacin, cotrimoxazole and roxythromycin are sensitive to many gram positive bacterial isolates. Conclusion: The present study has given the data of various bacteria encountered in the diabetic foot ulcer in the district of Kancheepuram, Tamil Nadu, India and its antibiotic sensitivity pattern. The results clearly reveal that there is no definite aetiology in diabetic foot infections. Many patients presented the infection with the involvement of many bacteria. Further it is evident that many bacteria are multi drug resistant and thus complicating the management of diabetic foot infections.
National Journal of Medical Research, 2012
Introduction: A Prospective study “Spectrum of Microbial flora in diabetic foot ulcer and its antibiotic sensitivity pattern” was carried out in a tertiary care hospital, Ahmedabad on 125 patients in which 85 were male patients and 40 were female patients. Material and Methods: Swabs samples were collected from the edge and margins of ulcers and organism were identified by gram staining culture and biochemical reactions. Results: Out of 125 specimens 108 specimens showed growth of organisms. Total 157 aerobic organisms were isolated from culture positive specimens. It represents an average of 1.25 organisms per case. Among these organisms, 130 gram negative and 27 gram positive organisms were isolated. Pseudomonas aeruginosa (30.57%) was predominant organism followed by Klebsiella spp. (22.29%). Staphylococcus aureus were 12.74% in which Methicillin resistant S. aureus (MRSA) was 55%. Conclusion: incidence of growth was 86.4% in which Pseudomonas aeruginosa (30.57%) is most common isolate. Organisms in mixed infections showed multidrug resistance as compared to single isolated strain. Diabetic foot infections are polymicrobial in nature. As the Wagner’s grade increased, the prevalence of isolates also increased.
International Journal of General Medicine & Surgery
Aim: To determine the microbiological profile and antibiotic susceptibility pattern of organisms in diabetic foot ulcers in our population. Settings and Design: A prospective observational study Material and Method: A prospective study was carried out on 62 patients of diabetic foot ulcers attending the endocrine OPD. The aerobic bacterial agents were isolated, and their antibiotic susceptibility pattern was studied. Results: Organisms were cultured from 90.32% cases of diabetic foot ulcers. Staphylococcus aureus (both MRSA and non-MRSA) was the commonest organism found in 36.77% cases followed by E. coli in 30.88%, Enterococci in 7.35%, Pseudomonas aerogenosa in 5.88% and Klebsiella in 5.88% of cases. Polymicrobial infection was seen in 9.68% cases. In 47.05% of the cases infections was due to gram negative organisms. Staphylococcus aureus was chiefly susceptible to levofloxacin, vancomycin, pipracillin tazobactum, linezolid while E. coli to levofloxacin, piperacillin tazobactum, and meropenem. Conclusion: Gram-negative organisms were most frequent type of isolate. Staphylococcus aureus was the most common type of bacteria. All bacterial isolate in our study have sensitivity for levofloxacin, pipracillin-tazobactum linezolid, and meropenem.
Pakistan Journal of Medical and Health Sciences, 2021
Background: Most of the diabetic patients present as diabetic foot in surgical outdoor and emergency department. Diabetic Foot infections are common and take long duration to be treated. Both Gram positive and Gram negative aerobic bacteria are involved in diabetic foot infection. Aim: To determine the frequency of most prevalent bacteria in wound of diabetic foot ulcers and to determine the antimicrobial susceptibility of isolated bacteria from diabetic foot ulcers so that an empirical antibiotics can be started before the report of culture and sensitivity. Methods: This cross sectional study was conducted in surgical unit 1 of Bahawal Victoria Hospital (BVH) Bahawalpur from 01-08-2020 to 31-07-2021. This study was conducted on 145 patients with diabetic foot ulcer from outdoor and emergency department of BVH. Data about patient’s demography, duration of diabetes, duration of DFUs, type of pathogen and its antibiotic susceptibility was entered on a proforma. Pus sample was obtained...
Bacterial Profile of Diabetic Foot Ulcer with duration and Types of Diabetes and Antibiotic Therapy
Pakistan Journal of Medical and Health Sciences
Objective: To find out the gram negative bacteria causing the diabetic foot ulcers and most effective antibiotic therapy. Study Design: Cross-sectional descriptive study Place and Duration of Study: Diabetic Center Hayatabad, Kabir Medical College Peshawar from 1st September 2021 to 28th February 2022. Methodology: Sixty nine admitted patients for the treatment of diabetes having type 1 diabetes mellitus and type 2 diabetes mellitus were enrolled. All the patients were on treatment of antibiotic such as gentacin, augmentin, amikacin and clindamycin. The specimens were analyze in microbiology laboratory and extracted by needle aspiration of material from the infected site and inoculate within 1hour after collection using gram staining smear for the detection or cytology of bacteria and its presence and absence in a specimens, for the isolation specimens were plated onto chocolate, phenyl ethyl alcohol (PEA) and MacConkey agar plate. To check the antibiotic susceptibility pattern Kirb...
2016
Foot infections are one of the foremost causes of mortality and morbidity in developing countries. Diabetes is the principal cause of nontraumatic lower extremity amputations and accounts for more than 85% of amputations. The determination of antimicrobial susceptibility of a clinical isolate is often essential for the most advantageous antimicrobial therapy of infection. Infection with multidrug resistant organisms (MDR) may enhance the length of hospital stay, cost of management and may cause further morbidity and mortality. Aims of this study were to determine the bacterial spectrum in diabetic foot ulcers and institute the appropriate antibiotic therapy to avoid further complications. Deep wound swab were cultured from 37 patients, who were receiving the treatment for the diabetic foot infections. Specimens were tested by Gram stain, culture & antibiotic sensitivity. Results showed that Gram negative aerobes were the most frequently isolated bacteria constituting 26 isolates (87...
International Journal of Current Microbiology and Applied Sciences, 2016
Diabetes mellitus is a metabolic disorder present with chronic hyperglycaemia and disturbances in carbohydrate, fat and protein metabolism. It is due to defects in insulin secretion, action or both. It manifested as damage or dysfunction in various organs like retina, kidney, Cardio vascular system, Cerebro vascular system, bone, joints & soft tissue like foot ulcer. It also includes physiological disturbances like sexual dysfunction. The major contributory factors for the high prevalence of the complications are; delayed diagnosis of diabetes, inadequate control of glycaemia,