Effect of copper nanoparticles on physico-chemical properties of chitosan and gelatin-based scaffold developed for skin tissue engineering application (original) (raw)
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Chitosan-based copper nanocomposite accelerates healing in excision wound model in rats
European Journal of Pharmacology, 2014
Copper possesses efficacy in wound healing which is a complex phenomenon involving various cells, cytokines and growth factors. Copper nanoparticles modulate cells, cytokines and growth factors involved in wound healing in a better way than copper ions. Chitosan has been shown to be beneficial in healing because of its antibacterial, antifungal, biocompatible and biodegradable polymeric nature. In the present study, chitosan-based copper nanocomposite (CCNC) was prepared by mixing chitosan and copper nanoparticles. CCNC was applied topically to evaluate its wound healing potential and to study its effects on some important components of healing process in open excision wound model in adult Wistar rats. Significant increase in wound contraction was observed in the CCNC-treated rats. The up-regulation of vascular endothelial growth factor (VEGF) and transforming growth factor-beta1(TGF-β 1) by CCNCtreatment revealed its role in facilitating angiogenesis, fibroblast proliferation and collagen deposition. The tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) were significantly decreased and increased, respectively, in CCNC-treated rats. Histological evaluation showed more fibroblast proliferation, collagen deposition and intact re-epithelialization in CCNC-treated rats. Immunohistochemistry of CD31 revealed marked increase in angiogenesis. Thus, we concluded that chitosan-based copper nanocomposite efficiently enhanced cutaneous wound healing by modulation of various cells, cytokines and growth factors during different phases of healing process. & 2014 Elsevier B.V. All rights reserved. body functions are compromised. To tide over such situations, a treatment modality is desired that speeds up the healing by actively regenerating the skin (dermis and epidermis). A recent study suggests that chitosan and its nanoparticles can inhibit skin aging and facilitates the extracellular matrix (ECM) in remodeling phase of wound healing (Leonida et al., 2011). The composites prepared using metal nanoparticles and polymers could find better utilization due to the enhanced antimicrobial Contents lists available at ScienceDirect
Journal of Chemical Engineering Research Updates, 2017
This study presents the findings of a research on fabricating composite nanofibrous mats including silver and copper nanoparticles for tissue engineering applications. For this purpose, two different types of silver nanoparticles (soluble starch capped silver nanoparticles, sodium alginate capped silver nanoparticles) and two different types of copper nanoparticles (soluble starch capped copper nanoparticles, sodium alginate capped copper nanoparticles) were successfully incorporated into polyvinyl alcohol (PVA) fibers through electrospinning process. Characterization studies with x-ray diffraction (XRD), scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FTIR), inductively coupled plasma spectrometer (ICP) were conducted to determine physical and structural properties of the obtained nanofiber mats. According to SEM analysis it was observed that interconnected and randomly-oriented nanofibers were successfully generated. Additionally, XRD and FTIR studies proved the existence of silver nanoparticles and hydroxapatite on the nanofiber mats immersed in simulated body fluid (SBF) for 7 days. The results indicated that long term silver ion release was achieved. Overall results showed that these nanofibrous mats can be good candidates as multifunctional scaffolds for tissue engineering applications.
Journal of Bioactive and Compatible Polymers; Sage Publications, 2023
The new sophisticated tissue engineering focused on producing nanocomposite with different morphologies for rapid tissue regeneration. In this case, utilizing nanotechnology with the incorporation of bio-based materials have achieved the interest of researchers. This research aims at developing hybrid bio-nano scaffold with collagen (Col), Nigella sativa (Ns), and chitosan (Cs) by a bi-layered green electrospinning on polyvinyl chloride (PVA) layer in a different ratio for tissue regeneration. Field emission electron microscopy (FE-SEM), fourier transform infrared spectroscopy (FTIR), moisture management properties, tensile properties, antibacterial activity, and wound healing assessment of the fabricated hybrid bio-nano scaffolds were employed to investigate the different properties of hybrid bio-nano scaffolds. The results exhibit that the sample with Col (50%) and Ns (25%), Cs (25%) has good fiber formation with a mean diameter of 381 ± 22 nm. This bio-nano scaffold has a porosity of 78 ± 6.9% and a fast absorbing-slow drying nature for providing a moist environment. The antibacterial zones of inhibition (ZOI) against Staphylococcus aureus and Escherichia coli were 10 ± 1.3 and 8 ± 0.9 mm respectively, and appeared to be adequate to inhibit bacterial action. The wound healing assessment states that 84 ± 3.8% of wound closure occurs in just 10 days, which is quicker (1.5 times) than the duration of a commercial bandage. All of the findings suggest that the bio-nano scaffold could be useful for skin tissue engineering.
Composites Part B: Engineering, 2019
Recently, nanofibrous-hydrogel composites are luring attention for tissue regeneration applications as they mimic soft-tissues' microstructure. Mostly, the electrospun nanofibers based on synthetic polymers such as Polycaprolactone (PCL) are placed in a crosslinked hydrogels. Due to hydrophobic nature of PCL, integration of these nanofibers with the hydrophilic hydrogels of matrix is not sufficient. In this study, we applied Poly (ethylene glycol) methyl ether methacrylate (PEGMA)-surface-modified PCL nanofibers within chitosan-gelatin hydrogels for skin regeneration applications. In addition, curcumin was loaded into PCL nanofibers due to its great impact on skin regeneration process. Fabricated nanofibrous-hydrogel scaffolds were characterized using scanning electron microscopy (SEM), porosimetery, Fourier transform infrared spectroscopy (FTIR), mechanical compression test, and water uptake studies. Curcumin release was investigated using UV/Vis spectrophotometry. In order to study biocompatibility of scaffolds MTT assay and cell culture was performed using L929 cells. FTIR spectra confirmed PEGMA modification of PCL nanofibers. Results of mechanical test determined that surface modified PCL nanofibers improved mechanical strength and modulus of scaffolds. Porosimetery studies showed proper porosity of scaffolds for skin regeneration from 90.43 to 71.48% and pore size of 101-256 μm. Biological test confirmed proper biocompatibility and good cell attachment to the scaffolds. Taken together, the chitosan/ gelatin hydrogel incorporating PEGMA modified PCL nanofibers containing curcumin shows great potential for skin regeneration.
Toxicology in Vitro, 2013
The increasing use of nano-sized materials in our environment, and in many consumer products, dictates new safety concerns. In particular, adequate experimental models are needed to evaluate skin toxicity of metal oxide ions, commonly found in cosmetic and dermatologic preparations. We have addressed the biological effects of topically applied copper oxide (CuO) nanoparticles in human skin organ cultures, using light and electron microscopy, and biochemical tests. Nanoparticles were more toxic than microsized particles, and their effects were stronger when supplied in growth medium than in topical application. Still topically applied CuO nanoparticles induced inflammatory cytokine secretion and necrosis, especially in epidermis deprived of its protective cornea. Since nanoparticle penetration was not seen, we propose that they may adhere to skin surface, react with the local acidic environment, and generate soluble ions that make their way to inner sites. This work illustrates the abilities of skin organ culture to evaluate the biological effects of topically-applied materials on skin in vitro.
International Journal of Applied Pharmaceutics, 2020
Objective: The purpose of this research was, synthesis of copper nanoparticles using environment friendly cementation method and evaluate their wound healing property on full-thickness excisional wound. Methods: Present study reports the synthesis of CNPs by single-step cementation method. Evaluation of CNPs was endorsed by morphological and chemical properties. Furthermore, CNPs was evaluated for its antibacterial potential and invitro hemocompatibility. Additionally, pharmacological evaluation of CNPs was assessed against excisional wound. Results: Characterization of final product indicate, particle size of CNPs were ranging from 100-150 nm. CNPs showed significant antibacterial activity (A= 2.1±0.1 mm, B =2.1±0.1 mm, C = 1.9±0.2 mm, at 10µg/ml), along with superior hemocompatibility (RBC cell survival 97±1 %). Further CNPs formulation shows increased level of anti-inflammatory cytokinin's (IL-10, 42.7%) as compared to standard (STD), vehicle control, and normal control groups, attributed to accelerated wound healing (p<0.05 vs STD). Conclusion: The consequences the present investigation endorse the accelerated wound healing potential of CNPs with its anti-inflammatory potential.
Journal of Biomaterials Science, Polymer Edition, 2023
The sophisticated new tissue regeneration focused on nanocomposite with different morphologies achieved through advanced manufacturing technology with the inclusion of bio-inscribed materials has piqued the research community’s interest. This research aims at developing hybrid bio-nanocomposites with collagen (Col), Nigella sativa (Ns) oil, and chitosan (Cs) by a bi-layered green electrospinning on polyvinyl chloride (PVA) layer in a different ratio for tissue regeneration. Fiber morphologies through scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), moisture management, tensile test, antibacterial activity, cell cytotoxicity, and wound healing through rabbit model of the fabricated hybrid bio-nanocomposites were investigated. It is worth noting that water-soluble Col (above 60% solution) does not form Taylor cones during electrospinning because unable to overcome the surface tension of the solution (viscosity) to form fibers. The results show that water-soluble Col (50% solution) to Cs (25% solution) and Ns (25% solution) have good fiber formation with a mean diameter of 384 ± 27 nm and a degree of porosity is 79%. The fast-absorbing and slow-drying hybrid bio-nanocomposites maintain a moist environment for wounds and allow gaseous exchange for cell migration and proliferation by the synergistic effects of bio-polymers. All of the biopolymers in bio-nanocomposite improve the H-bonds, which accounts for enough tensile strength to withstand cell pulling force. The anti-bacterial ZOI concentrations against S. aureus and E. coli were 10 and 8 mm, respectively, which appeared to be sufficient to inhibit bacterial action with 100% cell viability (cytotoxicity). The synergistic effects of Ns and Cs improve tissue regeneration, while native Col improves antibacterial activity, and the rabbit model achieves approximately 84% wound closure in only 10 days, which is 1.5 times faster than the control model. So, the fabricated hybrid bio-composites may be useful for skin tissue engineering.
Effect of copper nanoparticles on the cell viability of polymer composites
International Journal of Polymeric Materials and Polymeric Biomaterials, 2017
The goal of the present work to analyze the effect of copper nanoparticles (CNP) embedded in polymer matrices on the cell viability. Both calcium alginate (a natural hydrogel) and polypropylene (a synthetic thermoplastic) matrices were used. Cell viability was tested for cerebral cortex of normal (CNh) mouse fetuses and human osteosarcoma (SAOS-2). The viability strongly depends on the polymer characteristics as hydrogels presented cytotoxicity at concentrations higher than 0.5 wt% of CNP whereas polypropylene does not show any effect even at concentrations as high as 20 wt%. These results were explained by the different copper species released from the composites.
PREPARATION AND EVALUATION OF COPPER NANOPARTICLES LOADED HYDROGEL FOR BURNS Original Article
International Journal of Applied Pharmaceutics, 2021
Objective: The present study focuses on the development and optimization of copper nanoparticles (CNPs) loaded hydrogel for the treatment of dermal burn injuries. Methods: CNPs gel was prepared by dispersing the variable concentration of polyvinylpyrrolidone (PVP K30) and hydroxypropyl methylcellulose (HPMC) in distilled water, PEG 400, and copper nanoparticles. factor screening study was performed for identification of influential factors, followed by optimization study using three-factor Box-Behnken design. Results: Optimized nanogel formulation, when compared to normal control (NC), shows a significant reduction of pro-inflammatory cytokines (IL-6 = 39.74 % and TNF-α =49.37%) and increased level of anti-inflammatory cytokines (IL-10 = 30.90%), indicating reduced inflammation. Further, the wound closure rate of CNPs gel shows significant (12.27 %) wound closure as compared to the NC group and complete wound closure (100 %) on the 14th day, indicating accelerated wound healing. Conclusion: the present investigation endorses accelerated scar-free, accelerated wound healing potential of copper nanoparticles gel with antiinflammatory potential.
Properties of Collagen/Chitosan Scaffolds for Skin Tissue Engineering
Biopolymer blends between collagen and chitosan have the potential to produce cell scaffolds with biocompatible properties. In this study, porous scaffolds were fabricated by freeze drying the solution of collagen and chitosan and crosslinked by dehydrothermal treatment (DHT). Various types of scaffolds were prepared by varying compositions of collagen and chitosan. The scaffolds were fully characterized by Fourier transform infrared (FT-IR) spectroscopy. The results proved that collagen and chitosan scaffolds in all blending compositions contained only physical but not chemical interaction in molecular level. The compressive modulus from a universal mechanical testing machine decreased with increasing the compositions of chitosan. Equilibrium swelling ratios of approximately 6-8, carried out in phosphate buffered saline (PBS) at physiological pH (7.4) were found in case of collagen dominate scaffolds. The lysozyme biodegradation test demonstrated that the presence of chitosan could significantly prolong the biodegradation of collagen/chitosan scaffolds. The collagen/chitosan scaffold was more effective to promote and accelerate L929 cell proliferation, particularly for scaffolds containing 30% of chitosan. The results elucidated that the blends of collagen with chitosan have a high possibility to be applied as new materials for skin tissue engineering.