Pichia heedii, a New Species of Yeast Indigenous to Necrotic Cacti in the North American Sonoran Desert (original) (raw)
Related papers
International Journal of Systematic Bacteriology, 1984
We describe Pichia antillensis, a new species of yeast which is closely related to Pichia opuntiae. Pichia antillensis, 20 strains of which were isolated, is heterothallic and occurs in nature in both the haploid state and the diploid state. It produces asci with four hat-shaped spores, which are rapidly released upon maturity. The guanine-plus-cytosine content of its nuclear deoxyribonucleic acid is 33.4 to 33.7 mol%. The deoxyribonucleic acids of Pichia antillensis and Pichia opuntiae var. apuntiae show approximately 50% homology, but the two species are not interfertile. Moreover, the two species are separated geographically and by host plant. Pichia antillensis occurs principally in necrotic tissue of the columnar cactus Cephalocereus royenii in the West Indies, and Pichia opuntiae var. opuntiae occurs in Opuntia rots in Australia. The type strain of P. antillensis is strain UCD-FST 82-651A (= ATCC 56267 = CBS 7111).
International Journal of Systematic Bacteriology, 1985
We describe Pichiu deserficola and Candidu deserticola, two species that have as their habitats necrotic tissues of Opuntiu spp. and Sfenocereus spp., respectively. Pichiu deserticola, 21 strains of which were isolated, is homothallic and occurs in nature exclusively in the diploid state. It produces asci with two hat-shaped spores, which are rapidly released upon maturity. This species is nonfermentative and assimilates few carbon compounds. The guanine-plus-cytosine content range of the nuclear deoxyribonucleic acid (eight strains) is 27.4 to 28.4 mol%, and the average & standard deviation for eight strains is 27.8 k 0.4 mol%. Candida deserficola, 48 strains of which were isolated, has the same phenotypic properties and deoxyribonucleic acid base composition as P. deserficola, but lacks the ability to produce ascospores and is resistant to triterpene glycosides in growth media. The deoxyribonucleic acids of P. deserticola and C. deserh'cola show more than 96% homology, but the two species are separated geographically and by host plant. P. deserficola occurs in Opuntiu species in southern Arizona and Texas, whereas C. deserticola is found almost exclusively in columnar cacti of the genus Sfenocereus on certain Caribbean islands and in Baja California, Mexico. The type strain of P. deserficola is strain UCD-FST 83-467.3 (= ATCC 58091 = CBS 7119), and the type strain of C. deserticola is strain UCD-FST 76-355A (= ATCC 58088 = CBS 7121).
Pichia caribaea, a New Species of Yeast Occurring in Necrotic Tissue of Cacti in the Caribbean Area
International Journal of Systematic Bacteriology, 1992
We describe Pichia caribaea, a new species of yeast which is closely related to P. amethionina. P. caribaea, of which 92 strains were isolated, is heterothallic and occurs in nature in both the haploid state and the diploid state. It produces asci with four hat-shaped spores, which are gradually released upon maturity. P. caribaea occurs in rotting tissue of cereoid and opuntia cacti on various islands in the greater Caribbean area and on coastal land masses surrounding it. It resembles P. amethionina var. pachycereana in its assimilation pattern of carbon compounds but differs in its ability to ferment glucose strongly. The DNAs of P. caribaea and the two described varieties of P. amethionina show about 40% complementarity. The type strain of P. caribaea is strain UCD-FST 81-62 (= ATTC 76713 = CBS 7692). No. of P. Locality Host tissuea caribaea strains isolated
Pichia cactophila, a New Species of Yeast Found in Decaying Tissue of Cacti
International Journal of Systematic Bacteriology, 1978
Machaerocereus gum m o s u s Lophocereus schottii M. gummosus Pachycereus prin-M. gummosus glei Lemaireocereus th urberi M. gummosus M. gummosus L. thurberi M. gummosus L. thurberi M. gummosus M. gummosus P. pringlei L. thurberi M. gummosus M. gummosus L. thurberi M. gummosus P. pringlei M. gummosus P. pringlei Opuntia sp.
Pichia amethionina, a New Heterothallic Yeast Associated with the Decaying Stems of Cereoid Cacti
International Journal of Systematic Bacteriology, 1978
A new species of the genus Pichia has been recovered 38 times in the Sonoran Desert from "rot pockets" of cereoid cacti and from Drosophila species which utilize the cacti. We have named the species Pichia amethionina due to its absolute requirement for methionine or cysteine. P. amethionina is heterothallic and demonstrates an agglutination reaction when opposite mating types are mixed. Two varieties are designated based on the combination of mannitol assimilation and ecological habitat. P. amethionina var. amethionina, the type variety, was recovered from cacti in the subtribe Stenocereinae and cannot assimilate mannitol, whereas P. amethionina var. pachycereana was found in cacti of the subtribe Pachycereinae and can assimilate mannitol. Results are given which demonstrate that the assimilation of mannitol is controlled by a single genetic locus. An evaluation of the interfertility and postmating viability among the two varieties and possibly identical organisms was made. The base composition of the nuclear deoxyribonucleic acid (average of 10 strains) is 33.05 k 0.19 mol% guanine plus cytosine. The type strain of P. amethionina and of the type variety, P. amethionina var. amethionina, is UCD-FST 76-401B (=ATCC 36080 = CBS 6940). The type strain of P. amethionina var. pachycereana is UCD-FST 76-384A (=ATCC 36079 = CBS 6943).
Pichia barkeri, a New Yeast Species Occurring in Necrotic Tissue of Opuntia stricta
International Journal of Systematic Bacteriology, 1987
We describe Pichiu barkeri, a new cactophilic yeast species occurring in necrotic tissue of Opuntia stricta in New South Wales, Australia, and on several islands in the Caribbean Sea. The new species is homothallic and appears to occur in nature in the haploid state. After conjugation between a mother cell and a bud, four hat-shaped spores are produced that are rapidly released from the ascus. The range of guanine-plus-cytosine contents for the nuclear deoxyribonucleic acids of 22 strains is 35.7 to 36.6 mol% (average, 36.1 mol%; standard deviation, 0.2 mol%). The type strain of P. barkeri is strain UCD-FST 83-994.3 (ATCC 64111, CBS 7256) isolated at Discovery Bay, Jamaica.
Pichia insulana sp. nov., a novel cactophilic yeast from the Caribbean
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2010
A novel species of ascomycetous yeast, Pichia insulana sp. nov., is described from necrotic tissue of columnar cacti on Caribbean islands. P. insulana is closely related to and phenotypically very similar to Pichia cactophila and Pichia pseudocactophila. There are few distinctions between these taxa besides spore type, host preference and locality. Sporogenous strains of P. insulana that produce asci with four hat-shaped spores have been found only on Curaçao, whereas there was no evidence of sporogenous P. cactophila from that island. In addition, sequences of the D1/D2 fragment of the large-subunit rDNA from 12 Curaçao strains showed consistent differences from the sequences of the type strains of P. cactophila and P. pseudocactophila. The type strain of P. insulana is TSU00-106.5T (=CBS 11169T =UCD-FST 09-160T).
The Journal of General and Applied Microbiology, 2007
Two yeast strains (ST-235 and ST-237) isolated from insect frass collected in northeastern Thailand, were identified as Pichia acaciae and a hitherto undescribed species based on the sequence analysis of the D1/D2 domain of 26S rDNA, ITS regions, and DNA-DNA reassociation experiments. The latter strain, ST-237 (؍BCC 11769؍NBRC 103638؍JCM 12576), was described as Pichia koratensis sp. nov. Pichia koratensis is closely related to Pichia acaciae in the D1/D2 domain sequence of 26S rDNA and phenotypical characteristics but differs by 5 nucleotides (0.9%) from the type strain of P. acaciae and is clearly distinguished by the assimilation and fermentation of sucrose.
Brazilian journal of biology = Revista brasleira de biologia, 2005
The yeast species found in necrotic stems of three columnar cacti (Pilosocereus machrisii, Pilosocereus vilaboensis, and Praecereus euchlorus) at eight localities in Brazil were described and a similarity analysis using Sorensen distances was used to compare the composition of yeast species at these localities. Of 56 necrotic cactus stems sampled, 32 produced yeast colonies. Ten species of yeast or yeast-like microorganisms were identified from 53 isolates, with Pichia cactophila, Candida sonorensis, Geotrichum sp., and Sporopachydermia cereana being the most common. The remaining species occurred in low proportions in the cacti surveyed. The similarity analysis provided a dendogram (UPGMA) that clustered the yeast communities from different cactus species and indicated that host cactus species was unimportant in this clustering.