The efficacy of polycaprolactone/hydroxyapatite scaffold in combination with mesenchymal stem cells for bone tissue engineering (original) (raw)
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Journal of Biomedical Materials Research Part A, 2014
The aim of this study was to examine the differential capacity of isolated dental pulp stem cells (SHED) cultured onto four different scaffold materials. The differential potential of isolated SHED was examined on the following scaffolds: porous hydroxyapatite (pHAP) alone or combined with 3 polymers: polylactic-co-glycolic acid (PLGA), alginate and ethylene vinylacetate / ethylene vinylversatate (EVA/EVV). SHED were isolated by "outgrowth" method and characterized by the flow cytometry. Viability of cells grown with scaffolds was assessed by MTT and LDH assays.
Journal of Biomedical Materials Research Part A, 2010
Polycaprolactone (PCL) is a synthetic biodegradable polymer that has been approved for use as bone graft substitutes. In this study, PCL scaffolds incorporating hydroxyapatite (HAp) particles were fabricated by combined solvent casting and particulate leaching techniques. The average pore dimension was in the range of about 480-500 lm. The porosity, water absorption, and compressive modulus of the scaffold were evaluated. The responses of primary bone cells cultured on the PCL and PCL/HAp scaffolds were examined both in vitro and in vivo. In comparison with the cells grown on the PCL scaffold, those cultured on the PCL/HAp counterpart positively expressed the markers of osteogenic differentiation.
Polycaprolactone-Hydroxyapatite Composite Membrane Scaffolds for Bone Tissue Engineering
MRS Proceedings, 2013
ABSTRACTBone tissue engineering typically involves the use of porous, bioresorbable scaffolds to serve as temporary, three-dimensional scaffolds to guide cell attachment, differentiation, proliferation, and subsequent tissue regeneration. In this study we developed a composite membrane scaffold by phase inversion technique by using biodegradable polyester, Polycaprolactone (PCL), with hydroxyapatite (HA) in order to develop novel controlled nanostructured biomaterials for bone tissue engineering applications.After preparation, membrane scaffolds were characterized in order to evaluate its morphological, physico-chemical and mechanical properties and then used for the cell culture.Our experimental design consists to apply the knowledge of natural bone tissue remodelling in an in vitro membrane biohybrid system. We used human mesenchymal stem cells for culture in the membrane scaffolds inducing the differentiation in osteoblasts and human monocytes to trigger osteoclastogenesis. Osteo...
PloS one, 2011
The development of a new family of implantable bioinspired materials is a focal point of bone tissue engineering. Implant surfaces that better mimic the natural bone extracellular matrix, a naturally nano-composite tissue, can stimulate stem cell differentiation towards osteogenic lineages in the absence of specific chemical treatments. Herein we describe a bioactive composite nanofibrous scaffold, composed of poly-caprolactone (PCL) and nano-sized hydroxyapatite (HA) or beta-tricalcium phosphate (TCP), which was able to support the growth of human bone marrow mesenchymal stem cells (hMSCs) and guide their osteogenic differentiation at the same time. Morphological and physical/chemical investigations were carried out by scanning, transmission electron microscopy, Fourier-transform infrared (FTIR) spectroscopy, mechanical and wettability analysis. Upon culturing hMSCs on composite nanofibers, we found that the incorporation of either HA or TCP into the PCL nanofibers did not affect c...
RSC Advances, 2014
Biomimetic nanofibrous scaffolds combined with stem cells are promising for bone tissue engineering. In the present study, we have employed nano-hydroxyapatite (nHAp) contained polycaprolactone (PCL) nanofibers as a biomimetic nanofibrous scaffold, and mesenchymal stem cells derived from human induced pluripotent stem cells (hiPS-MSCs) as the novel stem cells sources. The response of hiPS-MSCs on the nanofibrous scaffolds in terms of cell proliferation and differentiation into the osteoblastic phenotype was investigated by XTT assay, scanning electron microscopy (SEM), osteogenic genes expression (runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), collagen I (COL1A1), and osteocalcin (OC)), ALP activity, and calcium deposition. It is clearly shown that the hiPS-MSCs attached, and proliferated on the nanofibrous scaffolds. Compared with PCL nanofibers without nHAp, the cells on the nHAp contained nanofibers demonstrated superior capabilites to differentiate to form calcified extracellular matrix. Together with gene expression, all of the results indicate the great potential of the hiPS-MSCs seeded biomimetic nanofibrous scaffolds for bone regeneration in the future.
Tissue Engineering Part A, 2010
Nanocrystalline hydroxyapatite (HAp) was synthesized from biowaste eggshells through sonication followed by the heat treatment. Calcium oxide as a precursor moiety for the synthesis of HAp was obtained through the heat treatment of eggshells at 900 C for 3 hr. The prepared HAp was characterized by Fouriertransform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM). The appearance of the FTIR absorption peaks in between at 516-1031 and 3,636 cm −1 shows phosphate and hydroxyl groups in prepared HAp, respectively. The XRD-patterns indicate the formation of HAp started within 5 min of sonication. The SEM morphologies suggested that the synthesized HAp was highly crystalline and compact. We tested the elemental analysis of the synthesized HAp through X-ray fluorescence spectroscopy and inductively coupled plasma mass spectroscopy. The higher Ca/P ratio has observed in heat-treated HAp. These results show that heat treatment facilitates the formation of highly crystalline and compact HAp. Cytotoxicity and osteogenic potential of human mesenchymal stem cells (hMSCs) were also evaluated in the presence of HAp. No significant cytotoxicity was noted in the presence of HAp, suggested their biocompatibility. Enhanced osteogenesis of hMSCs occurred with HAp powder, confirming the feasibility in the treatment of osteogenesis. Thus, synthesized HAp has the potential to use a biomaterial in tissue engineering applications for bone tissues. K E Y W O R D S eggshells, human mesenchymal stem cells, nano-hydroxyapatite, tissue engineering 1 | INTRODUCTION Hydroxyapatite (Ca 10 (PO 4) 6 (OH) 2 , HAp), consists of mainly calcium phosphate has received a significant amount of attention to the researchers for its biocompatibility, wastewater treatment, gas sensors, and catalyst potentials, and so forth (Bhatnagar, Kumar, &
Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine, 2016
Bone tissue engineering is strongly dependent on the use of three-dimensional scaffolds that can act as templates to accommodate cells and support tissue ingrowth. Despite its wide application in tissue engineering research, polycaprolactone presents a very limited ability to induce adhesion, proliferation and osteogenic cell differentiation. To overcome some of these limitations, different calcium phosphates, such as hydroxyapatite and tricalcium phosphate, have been employed with relative success. This work investigates the influence of nano-hydroxyapatite and micro-hydroxyapatite (nHA and mHA, respectively) particles on the in vitro biomechanical performance of polycaprolactone/hydroxyapatite scaffolds. Morphological analysis performed with scanning electron microscopy allowed us to confirm the production of polycaprolactone/hydroxyapatite constructs with square interconnected pores of approximately 350 µm and to assess the distribution of hydroxyapatite particles within the poly...
International Journal of Implant Dentistry, 2021
Background Finding a material that supports bone regeneration is the concern for many investigators. We supposed that a composite scaffold of poly(ε) caprolactone and β-tricalcium phosphate (PCL-TCP) would entail desirable characteristics of biocompatibility, bioresorbability, rigidity, and osteoconductivity for a proper guided bone regeneration. Furthermore, the incorporation of mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) would boost the bone regeneration. We conducted this study to evaluate the bone regeneration capacity of PCL-TCP scaffold that is loaded with MSCs and PRP. Materials and methods Five miniature pigs received 6 implants in 6 created-mandibular bony defects in the right and left lower premolar areas. The bony defects were managed according to the following three groups: the PCL-TCP scaffold loaded with MSCs and PRP (MSCs+PRP+PCL-TCP) group (n = 10), PCL-TCP scaffold loaded with PRP (PRP+PCL-TCP) group (n = 10), and PCL-TCP scaffold group (n = 10). Af...