TEER Measurement Techniques for In Vitro Barrier Model Systems (original) (raw)

Abstract

Transepithelial/transendothelial electrical resistance (TEER) is a widely accepted quantitative technique to measure the integrity of tight junction dynamics in cell culture models of endothelial and epithelial monolayers. TEER values are strong indicators of the integrity of the cellular barriers before they are evaluated for transport of drugs or chemicals. TEER measurements can be performed in real time without cell damage and generally are based on measuring ohmic resistance or measuring impedance across a wide spectrum of frequencies. The measurements for various cell types have been reported with commercially available measurement systems and also with custom-built microfluidic implementations. Some of the barrier models that have been widely characterized using TEER include the blood-brain barrier (BBB), gastrointestinal (GI) tract, and pulmonary models. Variations in these values can arise due to factors such as temperature, medium formulation, and passage number of cells. T...

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  144. ±79 Millicell-ERS/Endohm
  145. MDCK 1500 Impedance analyzer/Ag electrodes 140
  146. Caco-2 and Raji B 285 ± 76 Millicell-ERS/Endohm
  147. Caco-2 and Raji B 88 ± 27 Millicell-ERS system
  148. Caco-2, HT29 and Raji B 60 ± 17 Millicell-ERS system
  149. Caco-2 3000 -4000 Voltage-ohm meter/Ag electrodes 87 Primary Normal Human Bronchial Epithelial cells (NHBE) 3000 Millicell-ERS/Chopstick
  150. Human Nasal Epithelial (HNE) cells 3133 ± 665 EVOM/Endohm
  151. Human Bronchial Epithelial cell line (Calu-3) 300 -600 EVOM/STX2
  152. Human Bronchial Epithelial cell line (Calu-3) 1126 ± 222 EVOM/STX2
  153. Human Bronchial Epithelial cell line (Calu-3) 50 -60 (in microwells) EVOM/STX3
  154. Bronchial Epithelial cell line (NuLi-1) 685 ± 31 Ussing Chambers Systems 98
  155. Human alveolar epithelial (A549) cell line 152 Millicell-ERS system
  156. Calu-3, MRC5 and dendritic cells 200 EVOM/STX2
  157. Diseased bronchial epithelial cell line(CFBE41o-) 250 EVOM/STX2
  158. Alveolar Epithelia Primary rat alveolar type II cells 2000 Ussing Chamber 103 Primary rat alveolar type II cells 2320 ± 511 EVOM 105 Human Alveolar type-II Epithelial cells (HAEpC) 1000 -2000 EVOM 106 A549 45 -100 EVOM/STX2
  159. A549 140 Millicell-ERS system 112 Human pulmonary microvascular endothelial cells (HPMEC) and NCI-H441 565 ± 48 EVOM/STX2
  160. 108 Primary human type-II alveolar epithelial cells (HAT-II) and HPMEC 1730 ± 460 EVOM/STX2
  161. A549, macrophage like cells(THP-1), mast cells(HMC-1), endothelial cells(EA.hy 929) 250 EVOM/STX2
  162. HAEpC, macrophages, dendritic cells 1113 ± 30 Millicell-ERS system 113 Human alveolar epithelial and pulmonary microvascular endothelial cells > 800 Volt-Ohm meter -Ag/AgCl electrodes