Biosensors Based on Nano-Gold/Zeolite-Modified Ion Selective Field-Effect Transistors for Creatinine Detection (original) (raw)
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Direct evidence of advantage of using nanosized zeolite Beta for ISFET-based biosensor construction
Journal of Nanoparticle Research, 2013
Analytical characteristics of urease-and butyrylcholinesterase (BuChE)-based ion sensitive field-effect transistor (ISFET) biosensors were investigated by the incorporation of zeolite Beta nanoparticles with varying Si/Al ratios. The results obtained by the zeolite-modified ISFET transducers suggested that the Si/Al ratio strongly influenced the biosensor performances due to the electrostatic interactions among enzyme, substrate, and zeolite surface as well as the nature of the enzymatic reaction. Using relatively small nanoparticles (62.7 ± 10, 76.2 ± 10, and 77.1 ± 10 nm) rather than larger particles, that are widely used in the literature, allow us to produce more homogenous products which will give more control over the quantity of materials used on the electrode surface and ability to change solely Si/Al ratio without changing other parameters such as particle size, pore volume, and surface area. This should enable the investigation of the individual effect of changing acidic and electronic nature of this material on the biosensor characteristics. According to our results, high biosensor sensitivity is evident on nanosize and submicron size particles, with the former resulting in higher performance. The sensitivity of biosensors modified by zeolite particles is higher than that to the protein for both types of biosensors. Most significantly, our results show that the performance of constructed ISFET-type biosensors strongly depends on Si/Al ratio of employed zeolite Beta nanoparticles as well as the type of enzymatic reaction employed. All fabricated biosensors demonstrated high signal reproducibility and stability for both BuChE and urease.
Nanoscale Research Letters, 2016
In the work, silicalite particles were used for the surface modification of pH-sensitive field-effect transistors (pH-FETs) with the purpose of developing new creatinine-sensitive biosensor. Creatinine deiminase (CD) adsorbed on the surface of silicalite-coated pH-FET served as a bioselective membrane. The biosensor based on CD immobilized in glutaraldehyde vapor (GA) was taken as control. The creatinine-sensitive biosensor obtained by adsorption on silicalite was shown to have better analytical characteristics (two-to threefold increased sensitivity to creatinine, three-to fourfold lesser response and recovery times, a decrease of the detection limit of creatinine determination to 5 μM, etc.). Additionally, the biosensor based on CD adsorbed on silicalite (Sil/CD) was characterized by high signal reproducibility (relative standard deviation (RSD) for creatinine measurement = 2.6 %) and stability during storage (over 13 months). It was experimentally confirmed that the proposed biosensor was not sensitive either to high concentrations of sodium chloride or to the macromolecular protein fractions and can be used for direct quantitative analysis of creatinine in the blood serum. It was concluded that the method of CD adsorption on silicalite is well-suited for the creation of creatinine-sensitive biosensor with improved working characteristics.
Materials Science and Engineering: C, 2012
Urea and butyrylcholine chloride (BuChCl) biosensors were prepared by adsorption of urease and butyrylcholinesterase (BuChE) on heat-treated zeolite Beta crystals, which were incorporated into membranes deposited on ion-selective field-effect transistor (ISFET) surfaces. The responses, stabilities, and use for inhibition analysis of these biosensors were investigated. Different heat treatment procedures changed the amount of Brønsted acid sites without affecting the size, morphology, overall Si/Al ratio, external specific surface area, and the amount of terminal silanol groups in zeolite crystals. Upon zeolite incorporation the enzymatic responses of biosensors towards urea and BuChCl increased up to~2 and~5 times, respectively; and correlated with the amount of Brønsted acid sites. All biosensors demonstrated high signal reproducibility and stability for both urease and BuChE. The inhibition characteristics of urease and BuChE were also related to the Brønsted acidity. The pore volume and pore size increases measured for the heat-treated samples are very unlikely causes for the improvements observed in biosensors' performance, because urease and BuChE are approximately one order of magnitude larger than the resulting zeolite Beta pores. Overall, these results suggest that the zeolites incorporated into the biologically active membrane with enhanced Brønsted acidity can improve the performance of ISFET-based biosensors.
Nano- and microsized zeolites as a perspective material for potentiometric biosensors creation
Nanoscale Research Letters, 2015
A number of potentiometric biosensors based on coimmobilization of enzymes with different types of zeolite on pH-ion-sensitive field-effect transistor (ISFET) have been developed. Their working characteristics have been determined and compared. It was shown that clinoptilolite and zeolite Beta polymorph A (BEA) are more promising for creating biosensors than zeolite A. Changing the concentration of zeolite BEA in membranes, it is possible to extend the biosensor linear measurement range. The two-layer method of deposition of the enzyme with clinoptilolite was found to provide a significant increase in the biosensor sensitivity to substrates, whereas thermal modification of the zeolite BEA crystals can improve analytical characteristics of potentiometric biosensors for detection of toxic substances. These results show that it is possible to regulate the ISFET characteristics for different enzyme-based biosensors by tailoring the electrode surfaces via different zeolites. This makes zeolites strong candidates for integration into biosensors as ISFET modifiers.
Nanoscale Research Letters, 2014
A possibility of the creation of potentiometric biosensor by adsorption of enzyme urease on zeolite was investigated. Several variants of zeolites (nano beta, calcinated nano beta, silicalite, and nano L) were chosen for experiments. The surface of pH-sensitive field-effect transistors was modified with particles of zeolites, and then the enzyme was adsorbed. As a control, we used the method of enzyme immobilization in glutaraldehyde vapour (without zeolites). It was shown that all used zeolites can serve as adsorbents (with different effectiveness). The biosensors obtained by urease adsorption on zeolites were characterized by good analytical parameters (signal reproducibility, linear range, detection limit and the minimal drift factor of a baseline). In this work, it was shown that modification of the surface of pH-sensitive field-effect transistors with zeolites can improve some characteristics of biosensors.
Materials Science and Engineering: C, 2002
A creatinine-sensitive biosensor was developed using ion-sensitive field-effect transistors (ISFETs) as transducers and immobilised creatinine deiminase (CD) as bioselective element. CD was immobilised by UV photopolymerisation in poly(vinyl alcohol) containing styrylpyridinium (PVA/SbQ) membrane on the dielectric gate of the ISFET transducer. The developed ENFETs demonstrated a dependence of the sensor sensitivity on NaCl and buffer concentration. Minimal detection limit for creatinine determination in a model solution containing 144 mM NaCl and 5% bovine serum albumin (BSA), pH 7.4, was about 20 AM. Biosensor responses were quite reproducible and stable during continuous work at least for 25 h, and the relative standard deviation of the sensor response was approximately 3% (n = 45, for creatinine concentration of 0.1 mM). It was shown that creatinine-sensitive ENFETs demonstrated excellent storage stability for more than 6 months when kept dry at 4 -6 jC. D
Sensor Letters, 2011
Zeolite/enzyme nanobiocomposites of different compositions were tested in this work for the improvement of biosensor analytical characteristics. The bioselective element based on urease immobilized by cross-linking with glutaraldehyde was used as a model. The working characteristics of biosensors based on various zeolite/enzyme nanocomposites were examined and compared with those of urease-based biosensors. An optimal concentration of zeolytes beta (BEA) in bioselective elements is determined to be 1.5%. It ensures as wide linear range of measurement without remarkable loss in biosensor sensitivity to urea. The BEA zeolite-based biosensors were shown to have better working parameters in comparison with those based on zeolites A (LTA). A decrease in biosensor sensitivity to heavy metal ions was demonstrated for all zeolites used, which testifies to probable increase in stability of urea measurement in real environmental samples.
ZIF-8 Nanoparticles Based Electrochemical Sensor for Non-Enzymatic Creatinine Detection
Membranes, 2022
There is a consistent demand for developing highly sensitive, stable, cost-effective, and easy-to-fabricate creatinine sensors as creatinine is a reliable indicator of kidney and muscle-related disorders. Herein, we reported a highly sensitive and selective non-enzymatic electrochemical creatinine sensor via modifying poly(3,4 ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) coated indium tin oxide (ITO) substrate by zeolitic imidazolate framework-8 nanoparticles (ZIF-8 NPs). The topography, crystallinity, and composition of the sensing electrode were characterized by field emission scanning electron microscopy (FESEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The peroxidase-like activity of ZIF-8 nanoparticles enabled it to detect creatinine forming a zinc-creatinine composite. The electrochemical behavior and sensing performance were evaluated by amperometric and impedimetric analysis. The sensor obtained a suffic...
Biomarkers, 2019
An improved amperometric creatinine biosensor was fabricated that dependent on covalent immobilization of nanoparticles of creatininase (CANPs), creatinase (CINPs) and sarcosine oxidase (SOxNPs) onto gold electrode (AuE). The CANPs/CINPs/SOxNPs/AuE was characterized by scanning electron microscopy and cyclic voltammetry at various stages. The working electrode exhibited optimal response within 2s at a potential of 0.6V, against Ag/AgCl, pH 6.5 and 30°C. A linear relationship was observed between creatinine concentration range, 0.1-200μM and biosensor response i.e. current in mA, under optimum conditions. Biosensor offered a low detection limit of 0.1μM with long storage stability. Analytical recoveries of added creatinine in blood sera at 0.5mM and at 1.0mM concentrations, were 92.0% and 79.20% respectively. The precision i.e. within and between-batch coefficients of variation were 2.04% and 3.06% respectively. There was a good correlation (R 2 =0.99) between level of creatinine in sera, as calculated by the colorimetric method and present electrode. The CANPs/CINPs/SOxNPs/Au electrode was reused 200 times during the period of 180 days, with just 10% loss in its initial activity, while being stored at 4°C, when not in use.
Electroanalysis, 2013
Different modifications of the zeolites Na + -Beta and LTA were applied for improving the working characteristics of a urea biosensor. The bioselective membrane of the biosensor was based on urease and different zeolites co-immobilized with bovine serum albumin on the surface of a pH-FET. It was shown that the biosensors modified with the zeolites H + -Beta30 and H + -Beta50 are characterized by increased sensitivity to urea. The influence of the zeolite concentration on the sensitivity of the biosensors was studied. The optimal concentration of the zeolites H + -Beta30 and H + -Beta50 in the bioselective membrane was 15 %. Different variants of co-immobilization of urease and zeolite H + -Beta30 were studied and the optimal method was selected. Thus, a general conclusion is that the urea biosensor sensitivity can be improved using zeolite H + -Beta30 for urease immobilization in the bioselective membrane.