Benign nano-assemblages of silver induced by β galactosidase with augmented antimicrobial and industrial dye degeneration potential (original) (raw)

The fabrication of nanoparticles (NPs) has been a wide realm of studies focusing majorly on their dispersion and stabilization. The use of biological components as reducing agents has led to emergence of environment-friendly and cost-effective approaches of synthesis. The primary aim was synthesizing silver nanoparticles (AgNPs) mediated by enzyme β galactosidase. The surface plasmon resonance peaks of AgNPs were screened using ultraviolet-visible spectroscopy against varying time of synthesis and concentration of enzyme. The mean dimension was 12.89 ± 0.16 nm as determined by the transmission electron microscopy and selected area electron diffraction patterns. The obtained NPs were fine spherical and quasi-spherical assemblages as revealed by the scanning electron microscopy studies. Fourier transform infrared spectroscopy confirmed that β galactosidase contributed to the reduction and stabilization of the silver nanoparticles. The crystallinity and presence of elemental silver was...