Effect of Grape Seed Extract (vitis vinifera, sp) on Histopathology Appearance, CMYC and BCL-2 Gene Expression of AOM-DSS Induced Mice (original) (raw)

The use of Grape Seed Extract (GSE) as a dietary supplement to prevent cancer in Indonesia is increasing. The extract content such as resveratrol, quercetin, epigalochatecin and proanthocyanidin , are reported to increase apoptosis and prevents cancer cell proliferation which is shown by the expression level of cMyc and Bcl-2 gene. Azoxymethane (AOM) and Dextran sulfate sodium (DSS) are two chemical compounds used to induce cancer in mice. Methods: Fifteen female mice were divided into 3 groups, whose treatment was sequentially as follows: I. Induced by AOM- DSS; II. Induced by AOM-DSS and EBA; and III. Neither induced by AOM-DSS nor given EBA. Histopathology examination of the colons was presented in descriptive report. Analysis of c-Myc and Bcl-2 gene was performed; the gene expression level was determined using the Livak method and statistically analyzed using the Mann-Whitney test. Result: Dysplasia were shown in group I, mild dysplasia over the repair tissue in group II, and he...