Cytochrome C Oxidase Subunit I Gene of Mitochondrial DNA from Chelonia mydas in Qatar (original) (raw)
Abstract: DNA of Qatari C. mydas samples were successfully sequenced using the Folmer forward and reverse primers. The identification with BOLD of approximately 688 base pairs sequence revealed maximum homology (99.84%) with C. mydas , which is a species of turtle has been declared "extinct in wild" by IUCN. The next closest species 93.79% , was N. depressa which has a restricted geographical distribution and was reported to be endemic to the Australian continental shelf. The finding of characteristic species-specific COI sequences offers the prospect of identifying marine turtle species by using DNA barcode methodology as an auxiliary tool for taxonomy. This can also be used during field work when identifying lost nests, animals stranded on beaches or those killed as part of catching in fishery nets. A further use is in forensic litigation when turtle eggs or meat are the only available material and for the development of Qatar gene bank information.
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